PMID- 31275143
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1663-9812 (Print)
IS  - 1663-9812 (Electronic)
IS  - 1663-9812 (Linking)
VI  - 10
DP  - 2019
TI  - Evaluation of the Expression of Matrix Metalloproteinase-1 of Laryngeal Squamous 
      Cell Carcinoma by Ultrasound Molecular Imaging.
PG  - 655
LID - 10.3389/fphar.2019.00655 [doi]
LID - 655
AB  - Purpose: The aims of this study were to evaluate the expression of matrix 
      metalloproteinase-1 (MMP-1) on laryngeal squamous cell carcinoma (LSCC) and 
      improve the early diagnosis rate via ultrasound molecular imaging (USMI). 
      Methods: The microsized MMP-1-targeted microbubbles (MB(MMP-1)) and the control 
      MBs (MB(IgG)) based on perfluorocarbon-filled lipid-shelled MBs were constructed 
      and characterized. The in vitro binding experiment was performed with human 
      epidermoid laryngeal cancer cells (HEp-2) and tested the binding efficiency of 
      MB(MMP-1) and MB(IgG). In the in vivo study, the LSCC model was established in 10 
      mice. The MB(MMP-1) and MB(IgG) were randomly injected into tumor-bearing mice 
      via the tail vein at Day 7, Day 12, and Day 17 to dynamically evaluate the 
      differential targeted enhancement (dTE) signals via USMI. Subsequent 
      immunofluorescence analysis was used for confirmation of MMP-1 expression. 
      Result: The effective adhesion rate of MB(MMP-1) and MB(IgG) to HEp-2 was 298.42 
      +/- 16.57 versus 12.38 +/- 3.26 bubbles/per field in vitro experiment, which shows a 
      significant difference (P < 0.01). The in vivo ultrasound molecular imaging 
      (USMI) results demonstrated that dTE signal intensity from MB(MMP-1) was 
      significantly higher than that from the MB(IgG) at Day 7, Day 12, and Day 17 (Day 
      7, 41.21 +/- 15.00 versus 2.25 +/- 0.6 a.u., P < 0.05; Day 12, 124.64 +/- 5.19 versus 
      11.13 +/- 1.13 a.u., P < 0. 05; Day 17, 332.01 +/- 64.88 versus 42.99 +/- 11.9 a.u., P 
      < 0.01). Moreover, immunofluorescence analysis further confirmed the expression 
      of MMP-1 in LSCC with a gradual increase with the tumor growth. Conclusion: 
      MB(MMP-1) could be a potential probe that can be used in the early diagnosis of 
      LSCC by USMI.
FAU - Zhou, Yi
AU  - Zhou Y
AD  - GuangZhou University School of Medicine, GuangZhou, China.
AD  - Department of Ultrasound, The Second People's Hospital of Shenzhen, The First 
      Affiliated Hospital of Shenzhen University, Shenzhen, China.
FAU - Song, Zhuqing
AU  - Song Z
AD  - Department of Breast Surgery, Peking University Shenzhen Hospital, Shenzhen, 
      China.
FAU - Hu, Qiao
AU  - Hu Q
AD  - Department of Ultrasound, The People's Hospital of Guangxi Zhuang Autonomous 
      Region, Nanning, China.
FAU - Ji, Xiaojuan
AU  - Ji X
AD  - Department of Cardiology, Children's Hospital of Chongqing Medical University, 
      Chongqing, China.
FAU - Zheng, Hongyu
AU  - Zheng H
AD  - Department of Ultrasound, The People's Hospital of Guangxi Zhuang Autonomous 
      Region, Nanning, China.
FAU - Wang, Xiaoyan
AU  - Wang X
AD  - Department of Ultrasound, The People's Hospital of Guangxi Zhuang Autonomous 
      Region, Nanning, China.
FAU - Li, Zhenzhou
AU  - Li Z
AD  - Department of Ultrasound, The Second People's Hospital of Shenzhen, The First 
      Affiliated Hospital of Shenzhen University, Shenzhen, China.
LA  - eng
PT  - Journal Article
DEP - 20190619
PL  - Switzerland
TA  - Front Pharmacol
JT  - Frontiers in pharmacology
JID - 101548923
PMC - PMC6593083
OTO - NOTNLM
OT  - laryngeal squamous cell carcinoma
OT  - matrix metalloproteinase-1
OT  - targeted microbubbles
OT  - ultrasound molecular imaging
OT  - vasculogenic mimicry
EDAT- 2019/07/06 06:00
MHDA- 2019/07/06 06:01
PMCR- 2019/06/19
CRDT- 2019/07/06 06:00
PHST- 2019/01/30 00:00 [received]
PHST- 2019/05/20 00:00 [accepted]
PHST- 2019/07/06 06:00 [entrez]
PHST- 2019/07/06 06:00 [pubmed]
PHST- 2019/07/06 06:01 [medline]
PHST- 2019/06/19 00:00 [pmc-release]
AID - 10.3389/fphar.2019.00655 [doi]
PST - epublish
SO  - Front Pharmacol. 2019 Jun 19;10:655. doi: 10.3389/fphar.2019.00655. eCollection 
      2019.