PMID- 31275351
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1664-8021 (Print)
IS  - 1664-8021 (Electronic)
IS  - 1664-8021 (Linking)
VI  - 10
DP  - 2019
TI  - RNA-Seq Revealed Novel Non-proliferative Retinopathy Specific Circulating MiRNAs 
      in T2DM Patients.
PG  - 531
LID - 10.3389/fgene.2019.00531 [doi]
LID - 531
AB  - Background: Diabetic retinopathy (DR) is a common diabetes complication and was 
      considered as the major cause of blindness among young adults. MiRNAs are a group 
      of small non-coding RNAs regulating the expression of target genes and have been 
      reported to be associated with the development of DR in a variety of molecular 
      mechanisms. In this study, we aimed to identify miRNAs that are differentially 
      expressed (DE) in the serum of DR patients. Methods: We recruited 21 type 2 
      diabetes mellitus (T2DM) inpatients of Chinese Han ancestry, consisting of 10 
      non-proliferative DR patients (DR group) and 11 non-DR T2DM patients (NDR group). 
      MiRNA was extracted from fasting peripheral serum and quantified by RNA-seq. The 
      expression levels of miRNA were evaluated and compared between the two groups, 
      with adjustments made for age differences. The validated target genes of miRNAs 
      were subjected to a pathway analysis. We also constructed a weighted polygenic 
      risk score using the DE miRNA and evaluated its predictive power. Results: Five 
      miRNAs were DE between DR and NDR groups (p-Value </= 0.01, LFC >/= 2 or LFC </=-2). 
      These included miR-4448, miR-338-3p, miR-190a-5p, miR-485-5p, and miR-9-5p. In 
      total, these miRNAs were validated to regulate 55 target genes. Four target genes 
      were found to overlap with the NAD metabolism, sirtuin, and aging pathway, which 
      was thought to control the vascular growth and morphogenesis. The predictive 
      power of our polygenic risk score was apparently high (AUC = 0.909). However, it 
      needs to be interpreted with caution. Conclusion: In this study, we discovered 
      novel DR-specific miRNAs in human serum samples. These circulating miRNAs may 
      represent the pathological changes in the retina in response to diabetes and may 
      serve as non-invasive biomarkers for early DR risk prediction.
FAU - Li, Zimeng
AU  - Li Z
AD  - Department of Endocrinology, China-Japan Union Hospital, Jilin University, 
      Changchun, China.
FAU - Dong, Ying
AU  - Dong Y
AD  - Department of Radiotherapy, The Tumor Hospital of Jilin Province, Changchun, 
      China.
FAU - He, Chang
AU  - He C
AD  - Department of Genetics, College of Basic Medical Sciences, Jilin University, 
      Changchun, China.
AD  - Department of Molecular Biology, College of Basic Medical Sciences, Jilin 
      University, Changchun, China.
FAU - Pan, Xingchen
AU  - Pan X
AD  - Department of Genetics, College of Basic Medical Sciences, Jilin University, 
      Changchun, China.
AD  - Department of Molecular Biology, College of Basic Medical Sciences, Jilin 
      University, Changchun, China.
FAU - Liu, Dianyuan
AU  - Liu D
AD  - Department of Endocrinology, China-Japan Union Hospital, Jilin University, 
      Changchun, China.
FAU - Yang, Jianli
AU  - Yang J
AD  - Department of Genetics, College of Basic Medical Sciences, Jilin University, 
      Changchun, China.
FAU - Sun, Liankun
AU  - Sun L
AD  - Department of Pathophysiology, College of Basic Medical Sciences, Jilin 
      University, Changchun, China.
FAU - Chen, Peng
AU  - Chen P
AD  - Department of Genetics, College of Basic Medical Sciences, Jilin University, 
      Changchun, China.
AD  - Department of Pathology, College of Basic Medical Sciences, Jilin University, 
      Changchun, China.
FAU - Wang, Qing
AU  - Wang Q
AD  - Department of Endocrinology, China-Japan Union Hospital, Jilin University, 
      Changchun, China.
LA  - eng
PT  - Journal Article
DEP - 20190604
PL  - Switzerland
TA  - Front Genet
JT  - Frontiers in genetics
JID - 101560621
PMC - PMC6593299
OTO - NOTNLM
OT  - diabetes retinopathy
OT  - early prediction
OT  - microRNA
OT  - polygenic risk score
OT  - type 2 diabete mellitus
EDAT- 2019/07/06 06:00
MHDA- 2019/07/06 06:01
PMCR- 2019/06/04
CRDT- 2019/07/06 06:00
PHST- 2019/04/12 00:00 [received]
PHST- 2019/05/16 00:00 [accepted]
PHST- 2019/07/06 06:00 [entrez]
PHST- 2019/07/06 06:00 [pubmed]
PHST- 2019/07/06 06:01 [medline]
PHST- 2019/06/04 00:00 [pmc-release]
AID - 10.3389/fgene.2019.00531 [doi]
PST - epublish
SO  - Front Genet. 2019 Jun 4;10:531. doi: 10.3389/fgene.2019.00531. eCollection 2019.