PMID- 31276181
OWN - NLM
STAT- MEDLINE
DCOM- 20200316
LR  - 20200316
IS  - 2543-6767 (Electronic)
IS  - 0017-0011 (Linking)
VI  - 90
IP  - 6
DP  - 2019
TI  - GATA-3 Stimulates Proliferation of Endometriotic Cells.
PG  - 308-313
LID - 10.5603/GP.2019.0056 [doi]
AB  - OBJECTIVES: This study aimed to investigate whether there was a correlation 
      between the expression of GATA binding protein 3 (GATA-3) and the proliferation 
      of endometrium cells from women with endometriosis. MATERIAL AND METHODS: 
      Endometrial tissue was collected from 20 women with endometriosis (experimental 
      group) and 10 women without endometriosis (control group). The primary 
      endometrial cells were then cultured and identified. Each group of cells were 
      further divided into four groups. The control group was divided into control-no 
      treatment (C-N), control-negative control (C-NC, transfected with a scrambled 
      siRNA negative control), control-lentiviral transfection control (C-LC) and 
      control-GATA-3 lentiviral transfection (C-G3); the experimental group was divided 
      into experiment-no treatment (E-N), experiment-negative control (E-NC), 
      experiment-lentiviral transfection control (E-LC) and experiment-siGATA-3 
      lentiviral transfection (E-siG3). RT-PCR and western blot were used respectively 
      to detect the endometrium cells' GATA-3 mRNA and protein expression. Further, 
      Cell Counting Kit-8 was used to measure the endometrium cells' proliferation. 
      RESULTS: The relative expression of GATA-3 mRNA and protein in the E-N group was 
      significantly higher than in the D-N group and E-siG3 group (P < 0.05). The 
      relative cell proliferation rate in the C-G3 group at 48 h, 72 h and 96 h was 
      significantly higher than in the C-N, C-NC and C-LC groups (P < 0.05), while in 
      the experimental group, the proliferation rate of the E-siG3 group was 
      significantly lower than in the E-N, E-NC and E-LC groups (P < 0.05). 
      CONCLUSIONS: This study suggests the role of GATA-3 in promoting proliferation of 
      endometrial cells; therefore, GATA-3 may play an essential role in the 
      development of endometriosis.
FAU - Chen, Peng
AU  - Chen P
AD  - Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical 
      University, Shenyang, China.
FAU - Ren, Fang
AU  - Ren F
AD  - Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical 
      University, Shenyang, China.
FAU - Wang, Dan-Bo
AU  - Wang DB
AD  - Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical 
      University, Shenyang, China. wangdbsj@gmail.com.
AD  - Department of Gynecology, Cancer Hospital of China Medical University, Shenyang, 
      China. wangdbsj@gmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Poland
TA  - Ginekol Pol
JT  - Ginekologia polska
JID - 0374641
RN  - 0 (GATA3 Transcription Factor)
RN  - 0 (GATA3 protein, human)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Adult
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Endometriosis/diagnosis/*genetics/*pathology
MH  - Endometrium/*pathology
MH  - Female
MH  - GATA3 Transcription Factor/*genetics
MH  - Gene Expression
MH  - Humans
MH  - Middle Aged
MH  - RNA, Messenger/genetics
OTO - NOTNLM
OT  - GATA-3
OT  - endometriosis
OT  - endometriotic cell
OT  - proliferation
EDAT- 2019/07/06 06:00
MHDA- 2020/03/17 06:00
CRDT- 2019/07/06 06:00
PHST- 2019/03/20 00:00 [received]
PHST- 2019/06/05 00:00 [accepted]
PHST- 2019/06/04 00:00 [revised]
PHST- 2019/07/06 06:00 [entrez]
PHST- 2019/07/06 06:00 [pubmed]
PHST- 2020/03/17 06:00 [medline]
AID - VM/OJS/J/63648 [pii]
AID - 10.5603/GP.2019.0056 [doi]
PST - ppublish
SO  - Ginekol Pol. 2019;90(6):308-313. doi: 10.5603/GP.2019.0056.