PMID- 31276542
OWN - NLM
STAT- MEDLINE
DCOM- 20200221
LR  - 20231012
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 14
IP  - 7
DP  - 2019
TI  - Optimizing the alignment of thermoresponsive poly(N-isopropyl acrylamide) 
      electrospun nanofibers for tissue engineering applications: A factorial design of 
      experiments approach.
PG  - e0219254
LID - 10.1371/journal.pone.0219254 [doi]
LID - e0219254
AB  - Thermoresponsive polymers, such as poly(N-isopropyl acrylamide) (PNIPAM), have 
      been identified and used as cell culture substrates, taking advantage of the 
      polymer's lower critical solution temperature (LCST) to mechanically harvest 
      cells. This technology bypasses the use of biochemical enzymes that cleave 
      important cell-cell and cell-matrix interactions. In this study, the process of 
      electrospinning is used to fabricate and characterize aligned PNIPAM nanofiber 
      scaffolds that are biocompatible and thermoresponsive. Nanofiber scaffolds 
      produced by electrospinning possess a 3D architecture that mimics native 
      extracellular matrix, providing physical and chemical cues to drive cell function 
      and phenotype. We present a factorial design of experiments (DOE) approach to 
      systematically determine the effects of different electrospinning process 
      parameters on PNIPAM nanofiber diameter and alignment. Results show that high 
      molecular weight PNIPAM can be successfully electrospun into both random and 
      uniaxially aligned nanofiber mats with similar fiber diameters by simply altering 
      the speed of the rotating mandrel collector from 10,000 to 33,000 RPM. PNIPAM 
      nanofibers were crosslinked with OpePOSS, which was verified using FTIR. The 
      mechanical properties of the scaffolds were characterized using dynamic 
      mechanical analysis, revealing an order of magnitude difference in storage 
      modulus (MPa) between cured and uncured samples. In summary, cross-linked PNIPAM 
      nanofiber scaffolds were determined to be stable in aqueous culture, 
      biocompatible, and thermoresponsive, enabling their use in diverse cell culture 
      applications.
FAU - Young, Rachel E
AU  - Young RE
AD  - Department of Chemical and Biomolecular Engineering, Lafayette College, Easton, 
      Pennsylvania, United States of America.
FAU - Graf, Jodi
AU  - Graf J
AD  - Department of Chemical and Biomolecular Engineering, Lafayette College, Easton, 
      Pennsylvania, United States of America.
FAU - Miserocchi, Isabella
AU  - Miserocchi I
AD  - Department of Chemical and Biomolecular Engineering, Lafayette College, Easton, 
      Pennsylvania, United States of America.
FAU - Van Horn, Ryan M
AU  - Van Horn RM
AUID- ORCID: 0000-0002-1065-7378
AD  - Department of Chemical and Biomolecular Engineering, Lafayette College, Easton, 
      Pennsylvania, United States of America.
FAU - Gordon, Melissa B
AU  - Gordon MB
AUID- ORCID: 0000-0001-5881-3432
AD  - Department of Chemical and Biomolecular Engineering, Lafayette College, Easton, 
      Pennsylvania, United States of America.
FAU - Anderson, Christopher R
AU  - Anderson CR
AD  - Department of Chemical and Biomolecular Engineering, Lafayette College, Easton, 
      Pennsylvania, United States of America.
FAU - Sefcik, Lauren S
AU  - Sefcik LS
AUID- ORCID: 0000-0003-1130-2605
AD  - Department of Chemical and Biomolecular Engineering, Lafayette College, Easton, 
      Pennsylvania, United States of America.
LA  - eng
PT  - Journal Article
DEP - 20190705
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Acrylamides)
RN  - 0 (Acrylic Resins)
RN  - 0 (Biocompatible Materials)
RN  - 0 (Polymers)
RN  - 25189-55-3 (poly-N-isopropylacrylamide)
SB  - IM
MH  - Acrylamides/chemistry
MH  - Acrylic Resins/*chemistry
MH  - Biocompatible Materials/chemistry
MH  - Extracellular Matrix
MH  - Nanofibers/*chemistry
MH  - Polymers/chemistry
MH  - Tissue Engineering/*methods
MH  - Tissue Scaffolds/chemistry
PMC - PMC6611625
COIS- The authors have declared that no competing interests exist.
EDAT- 2019/07/06 06:00
MHDA- 2020/02/23 06:00
PMCR- 2019/07/05
CRDT- 2019/07/06 06:00
PHST- 2018/12/21 00:00 [received]
PHST- 2019/06/19 00:00 [accepted]
PHST- 2019/07/06 06:00 [entrez]
PHST- 2019/07/06 06:00 [pubmed]
PHST- 2020/02/23 06:00 [medline]
PHST- 2019/07/05 00:00 [pmc-release]
AID - PONE-D-18-36590 [pii]
AID - 10.1371/journal.pone.0219254 [doi]
PST - epublish
SO  - PLoS One. 2019 Jul 5;14(7):e0219254. doi: 10.1371/journal.pone.0219254. 
      eCollection 2019.