PMID- 31281325
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231012
IS  - 1664-462X (Print)
IS  - 1664-462X (Electronic)
IS  - 1664-462X (Linking)
VI  - 10
DP  - 2019
TI  - Arabidopsis NSE4 Proteins Act in Somatic Nuclei and Meiosis to Ensure Plant 
      Viability and Fertility.
PG  - 774
LID - 10.3389/fpls.2019.00774 [doi]
LID - 774
AB  - The SMC 5/6 complex together with cohesin and condensin is a member of the 
      structural maintenance of chromosome (SMC) protein family. In non-plant organisms 
      SMC5/6 is engaged in DNA repair, meiotic synapsis, genome organization and 
      stability. In plants, the function of SMC5/6 is still enigmatic. Therefore, we 
      analyzed the crucial delta-kleisin component NSE4 of the SMC5/6 complex in the model 
      plant Arabidopsis thaliana. Two functional conserved Nse4 paralogs (Nse4A and 
      Nse4B) are present in A. thaliana, which may have evolved via gene 
      subfunctionalization. Due to its high expression level, Nse4A seems to be the 
      more essential gene, whereas Nse4B appears to be involved mainly in seed 
      development. The morphological characterization of A. thaliana T-DNA mutants 
      suggests that the NSE4 proteins are essential for plant growth and fertility. 
      Detailed investigations in wild-type and the mutants based on live cell imaging 
      of transgenic GFP lines, fluorescence in situ hybridization (FISH), 
      immunolabeling and super-resolution microscopy suggest that NSE4A acts in several 
      processes during plant development, such as mitosis, meiosis and chromatin 
      organization of differentiated nuclei, and that NSE4A operates in a cell 
      cycle-dependent manner. Differential response of NSE4A and NSE4B mutants after 
      induced DNA double strand breaks (DSBs) suggests their involvement in DNA repair 
      processes.
FAU - Zelkowski, Mateusz
AU  - Zelkowski M
AD  - Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, 
      Germany.
FAU - Zelkowska, Katarzyna
AU  - Zelkowska K
AD  - Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, 
      Germany.
FAU - Conrad, Udo
AU  - Conrad U
AD  - Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, 
      Germany.
FAU - Hesse, Susann
AU  - Hesse S
AD  - Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, 
      Germany.
FAU - Lermontova, Inna
AU  - Lermontova I
AD  - Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, 
      Germany.
AD  - Plant Cytogenomics Research Group, Central European Institute of Technology, 
      Masaryk University, Brno, Czechia.
FAU - Marzec, Marek
AU  - Marzec M
AD  - Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, 
      Germany.
AD  - Department of Genetics, Faculty of Biology and Environmental Protection, 
      University of Silesia, Katowice, Poland.
FAU - Meister, Armin
AU  - Meister A
AD  - Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, 
      Germany.
FAU - Houben, Andreas
AU  - Houben A
AD  - Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, 
      Germany.
FAU - Schubert, Veit
AU  - Schubert V
AD  - Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, 
      Germany.
LA  - eng
PT  - Journal Article
DEP - 20190620
PL  - Switzerland
TA  - Front Plant Sci
JT  - Frontiers in plant science
JID - 101568200
PMC - PMC6596448
OTO - NOTNLM
OT  - Arabidopsis thaliana
OT  - NSE4 delta-kleisin
OT  - SMC5/6 complex
OT  - meiosis
OT  - mitosis
OT  - nucleus
OT  - phylogeny
OT  - super-resolution microscopy
EDAT- 2019/07/10 06:00
MHDA- 2019/07/10 06:01
PMCR- 2019/01/01
CRDT- 2019/07/09 06:00
PHST- 2019/02/22 00:00 [received]
PHST- 2019/05/28 00:00 [accepted]
PHST- 2019/07/09 06:00 [entrez]
PHST- 2019/07/10 06:00 [pubmed]
PHST- 2019/07/10 06:01 [medline]
PHST- 2019/01/01 00:00 [pmc-release]
AID - 10.3389/fpls.2019.00774 [doi]
PST - epublish
SO  - Front Plant Sci. 2019 Jun 20;10:774. doi: 10.3389/fpls.2019.00774. eCollection 
      2019.