PMID- 31281384
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220409
IS  - 1687-966X (Print)
IS  - 1687-9678 (Electronic)
VI  - 2019
DP  - 2019
TI  - Influence of Lineage-Negative Stem Cell Therapy on Articulatory Functions in ALS 
      Patients.
PG  - 7213854
LID - 10.1155/2019/7213854 [doi]
LID - 7213854
AB  - INTRODUCTION: Amyotrophic lateral sclerosis (ALS) is a fatal, neurodegenerative 
      disease, leading to loss of muscle strength and motor control. Impaired speech 
      and swallowing lower the quality of life and consequently may induce acute 
      respiratory failure. Bone marrow-derived stem and progenitor cells (SPCs) may be 
      a valuable source of trophic factors. In this study, we assessed whether adjuvant 
      cellular therapy could affect the levels of selected neurotrophins and 
      proinflammatory factors in the cerebrospinal fluid (CSF) and subsequently prevent 
      the deterioration of articulation. MATERIALS AND METHODS: The study group 
      consisted of 32 patients with sporadic ALS who underwent autologous 
      lineage-negative (Lin(-)) stem cell intrathecal administration to the spinal 
      canal. Lin(-) cells were aspirated from the bone marrow and isolated using 
      immunomagnetic beads and a lineage cell depletion kit. Patients were examined for 
      articulatory functions by means of the Voice Handicap Index (VHI) questionnaire 
      and Frenchay Dysarthria Assessment (FDA). In parallel, we carried out the 
      analysis of selected trophic and proinflammatory factors in CSF utilizing 
      multiplex fluorescent bead-based immunoassays. RESULTS: Of the 32 patients who 
      received the Lin(-) progenitor cell therapy, 6 (group I) showed improvement in 
      articulatory functions, 23 remained stable (group II), and 3 deteriorated (group 
      III) on the 28(th) day. The improvement was particularly noticeable in a better 
      cough reflex, laryngeal time, and dribble reflex. A statistically significant 
      lower level of brain-derived neurotrophic factor (BDNF) was observed on day 0 in 
      group I compared to group II. The CSF concentrations of C-reactive protein (CRP) 
      in group I significantly decreased 7 days after Lin(-) SPC transplantation. On 
      the contrary, a significant increase in the tumor necrosis factor receptor 
      (TNF-R) level was confirmed among patients from group I with improvement of 
      dribble and coughing reflex, tongue movements, and respiration on the 7(th) day, 
      as well as on day 28 including dribble reflex solely. CONCLUSIONS: An application 
      of Lin(-) stem cells could potentate the beneficial humoral effect. The 
      prevention of deterioration of articulatory functions in ALS patients after 
      applying adjuvant Lin(-) stem cell therapy seems to be promising. Although the 
      procedure is safe and feasible, it requires further in-depth studies.
FAU - Pawlukowska, Wioletta
AU  - Pawlukowska W
AUID- ORCID: 0000-0002-6536-2905
AD  - Department of Medical Rehabilitation and Clinical Physiotherapy, Pomeranian 
      Medical University, Szczecin, Poland.
AD  - Department of Neurology, Pomeranian Medical University, Szczecin, Poland.
FAU - Baumert, Bartlomiej
AU  - Baumert B
AD  - Department of General Pathology, Pomeranian Medical University, Szczecin, Poland.
FAU - Golab-Janowska, Monika
AU  - Golab-Janowska M
AUID- ORCID: 0000-0002-4934-9698
AD  - Department of Neurology, Pomeranian Medical University, Szczecin, Poland.
FAU - Sobus, Anna
AU  - Sobus A
AD  - Department of General Pathology, Pomeranian Medical University, Szczecin, Poland.
FAU - Welnicka, Agnieszka
AU  - Welnicka A
AD  - Department of Neurology, Pomeranian Medical University, Szczecin, Poland.
FAU - Meller, Agnieszka
AU  - Meller A
AD  - Department of Neurology, Pomeranian Medical University, Szczecin, Poland.
FAU - Machowska-Sempruch, Karolina
AU  - Machowska-Sempruch K
AD  - Department of Neurology, Pomeranian Medical University, Szczecin, Poland.
FAU - Zawislak, Alicja
AU  - Zawislak A
AD  - Department of General Pathology, Pomeranian Medical University, Szczecin, Poland.
FAU - Luczkowska, Karolina
AU  - Luczkowska K
AUID- ORCID: 0000-0003-4008-7473
AD  - Department of General Pathology, Pomeranian Medical University, Szczecin, Poland.
FAU - Milczarek, Slawomir
AU  - Milczarek S
AD  - Department of General Pathology, Pomeranian Medical University, Szczecin, Poland.
FAU - Osekowska, Bogumila
AU  - Osekowska B
AD  - Department of General Pathology, Pomeranian Medical University, Szczecin, Poland.
FAU - Paczkowska, Edyta
AU  - Paczkowska E
AUID- ORCID: 0000-0001-7052-9741
AD  - Department of General Pathology, Pomeranian Medical University, Szczecin, Poland.
FAU - Rotter, Iwona
AU  - Rotter I
AUID- ORCID: 0000-0002-4337-6476
AD  - Department of Medical Rehabilitation and Clinical Physiotherapy, Pomeranian 
      Medical University, Szczecin, Poland.
FAU - Nowacki, Przemyslaw
AU  - Nowacki P
AD  - Department of Neurology, Pomeranian Medical University, Szczecin, Poland.
FAU - Machalinski, Boguslaw
AU  - Machalinski B
AUID- ORCID: 0000-0002-6013-0419
AD  - Department of General Pathology, Pomeranian Medical University, Szczecin, Poland.
LA  - eng
PT  - Journal Article
DEP - 20190602
PL  - United States
TA  - Stem Cells Int
JT  - Stem cells international
JID - 101535822
PMC - PMC6589318
EDAT- 2019/07/10 06:00
MHDA- 2019/07/10 06:01
PMCR- 2019/06/02
CRDT- 2019/07/09 06:00
PHST- 2019/02/26 00:00 [received]
PHST- 2019/04/17 00:00 [revised]
PHST- 2019/05/08 00:00 [accepted]
PHST- 2019/07/09 06:00 [entrez]
PHST- 2019/07/10 06:00 [pubmed]
PHST- 2019/07/10 06:01 [medline]
PHST- 2019/06/02 00:00 [pmc-release]
AID - 10.1155/2019/7213854 [doi]
PST - epublish
SO  - Stem Cells Int. 2019 Jun 2;2019:7213854. doi: 10.1155/2019/7213854. eCollection 
      2019.