PMID- 31281453
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 18
IP  - 1
DP  - 2019 Jul
TI  - Inhibition of TLR4 inhibits allergic responses in murine allergic rhinitis by 
      regulating the NF-kappaB pathway.
PG  - 761-768
LID - 10.3892/etm.2019.7631 [doi]
AB  - The present study investigated the underlying mechanisms and effects of toll-like 
      receptor 4 (TLR4) on a mouse model of allergic rhinitis (AR). An ovalbumin 
      (OVA)-induced mouse model of AR was treated with TLR4-short hairpin RNA (shRNA). 
      Allergic symptoms were then subsequently assessed. Protein levels of OVA-specific 
      immunoglobulin E (IgE), eosinophil cation protein (ECP), leukotriene C4 (LTC4) 
      and prostaglandin D2 (PGD2) in mice serum and nasal lavage fluid, as well as 
      various inflammatory cytokine mediators in mice serum, were determined by ELISA. 
      Protein level detection was performed using reverse transcription-quantitative 
      PCR and western blot analysis. The results revealed that TLR4 was highly 
      expressed in the nasal mucosa of AR mice. TLR4 inhibition significantly relieved 
      OVA-induced AR symptoms. Relief of symptoms was evidenced by a decreased 
      frequency of sneezing and nose friction, reduced levels of OVA-specific IgE, ECP, 
      LTC4, PGD2, less inflammatory cells and decreased levels of T-helper 2 type 
      cytokines. In addition, the data indicated that OVA-induced activation of the 
      NF-kappaB pathway was repressed by TLR4-shRNA. The results of the current study 
      indicate that TLR4 may be a promising therapeutic target of AR.
FAU - Xu, Hangyu
AU  - Xu H
AD  - Department of Otolaryngology, Taizhou Central Hospital, Taizhou University 
      Hospital, Taizhou, Zhejiang 318000, P.R. China.
FAU - Shu, Hairong
AU  - Shu H
AD  - Department of Otolaryngology, Taizhou Central Hospital, Taizhou University 
      Hospital, Taizhou, Zhejiang 318000, P.R. China.
FAU - Zhu, Jie
AU  - Zhu J
AD  - Medical Test Center, Taizhou Central Hospital, Taizhou University Hospital, 
      Taizhou, Zhejiang 318000, P.R. China.
FAU - Song, Jianxin
AU  - Song J
AD  - Department of Otolaryngology, Taizhou Central Hospital, Taizhou University 
      Hospital, Taizhou, Zhejiang 318000, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20190529
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC6580108
OTO - NOTNLM
OT  - allergic response
OT  - allergic rhinitis
OT  - nuclear factor kappaB pathway
OT  - toll-like receptor 4
EDAT- 2019/07/10 06:00
MHDA- 2019/07/10 06:01
PMCR- 2019/05/29
CRDT- 2019/07/09 06:00
PHST- 2018/10/20 00:00 [received]
PHST- 2019/03/29 00:00 [accepted]
PHST- 2019/07/09 06:00 [entrez]
PHST- 2019/07/10 06:00 [pubmed]
PHST- 2019/07/10 06:01 [medline]
PHST- 2019/05/29 00:00 [pmc-release]
AID - ETM-0-0-7631 [pii]
AID - 10.3892/etm.2019.7631 [doi]
PST - ppublish
SO  - Exp Ther Med. 2019 Jul;18(1):761-768. doi: 10.3892/etm.2019.7631. Epub 2019 May 
      29.