PMID- 31282367
OWN - NLM
STAT- MEDLINE
DCOM- 20190919
LR  - 20190919
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 235
DP  - 2019 Aug
TI  - Virulent Lena strain induced an earlier and stronger downregulation of CD163 in 
      bronchoalveolar lavage cells.
PG  - 101-109
LID - S0378-1135(19)30539-5 [pii]
LID - 10.1016/j.vetmic.2019.06.011 [doi]
AB  - Highly virulent porcine reproductive and respiratory syndrome virus (PRRSV) 
      strains have increasingly overwhelmed Asia and Europe in recent years. This study 
      aims to compare the clinical signs, gross and microscopic findings as well as the 
      expression of CD163 within live pulmonary alveolar macrophages (PAMs) from 
      bronchoalveolar lavage fluid (BALF) of pigs experimentally infected with two 
      PRRSV strains of different virulence. Pigs were infected with either a subtype 1 
      PRRSV-1 3249 strain or a subtype 3 PRRSV-1 Lena strain and consecutively 
      euthanized at 1, 3, 6, 8 and 13 days post-inoculation. Clinical signs were 
      reported daily and BALF and lung tissue samples were collected at the different 
      time-points and accordingly processed for their analysis. Pigs infected with Lena 
      strain exhibited greater clinical signs as well as gross and microscopic lung 
      scores compared to 3249-infected pigs. A decreased frequency of PAMs from BALF 
      was observed early in pigs infected with Lena strain. Moreover, the frequency and 
      median fluorescence intensity (MFI) of CD163 within PAMs were much lower in 
      Lena-infected pigs than in 3249-infected pigs. This downregulation in CD163 was 
      also observed in lung sections after the assessment of macrophages expressing 
      CD163 by means of immunohistochemistry. This outcome may result from the effect 
      of PRRSV replication, PRRSV-induced inflammation, the influx of immature 
      macrophages to restore lung homeostasis and/or the evidence of CD163(low) cells 
      after CD163(+) cells decrease in BALF.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Rodriguez-Gomez, Irene M
AU  - Rodriguez-Gomez IM
AD  - Department of Anatomy and Comparative Pathology, Faculty of Veterinary Medicine, 
      University of Cordoba, International Excellence Agrifood Campus 'ceiA3', Cordoba, 
      Spain. Electronic address: v22rogoi@uco.es.
FAU - Sanchez-Carvajal, Jose M
AU  - Sanchez-Carvajal JM
AD  - Department of Anatomy and Comparative Pathology, Faculty of Veterinary Medicine, 
      University of Cordoba, International Excellence Agrifood Campus 'ceiA3', Cordoba, 
      Spain.
FAU - Pallares, Francisco J
AU  - Pallares FJ
AD  - Department of Anatomy and Comparative Pathology, Faculty of Veterinary Medicine, 
      University of Murcia, Mare Nostrum Excellence Campus, Murcia, Spain.
FAU - Mateu, Enric
AU  - Mateu E
AD  - Department of Animal Health and Anatomy, Faculty of Veterinary Medicine, 
      Autonomous University of Barcelona, Bellaterra, Barcelona, Spain.
FAU - Carrasco, Librado
AU  - Carrasco L
AD  - Department of Anatomy and Comparative Pathology, Faculty of Veterinary Medicine, 
      University of Cordoba, International Excellence Agrifood Campus 'ceiA3', Cordoba, 
      Spain.
FAU - Gomez-Laguna, Jaime
AU  - Gomez-Laguna J
AD  - Department of Anatomy and Comparative Pathology, Faculty of Veterinary Medicine, 
      University of Cordoba, International Excellence Agrifood Campus 'ceiA3', Cordoba, 
      Spain.
LA  - eng
PT  - Journal Article
DEP - 20190612
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CD163 antigen)
RN  - 0 (Receptors, Cell Surface)
SB  - IM
MH  - Animals
MH  - Antigens, CD/*genetics/immunology
MH  - Antigens, Differentiation, Myelomonocytic/*genetics/immunology
MH  - Bronchoalveolar Lavage Fluid/cytology
MH  - Bronchopneumonia/*veterinary/virology
MH  - Down-Regulation
MH  - Female
MH  - Lung/cytology/virology
MH  - Macrophages, Alveolar/*immunology/virology
MH  - Male
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology
MH  - Porcine respiratory and reproductive syndrome virus/immunology/*pathogenicity
MH  - Receptors, Cell Surface/*genetics/immunology
MH  - Swine
MH  - Virulence
OTO - NOTNLM
OT  - BALF
OT  - CD163
OT  - Lena
OT  - PAMs
OT  - PRRSV-1
EDAT- 2019/07/10 06:00
MHDA- 2019/09/20 06:00
CRDT- 2019/07/09 06:00
PHST- 2019/05/01 00:00 [received]
PHST- 2019/06/06 00:00 [revised]
PHST- 2019/06/12 00:00 [accepted]
PHST- 2019/07/10 06:00 [pubmed]
PHST- 2019/09/20 06:00 [medline]
PHST- 2019/07/09 06:00 [entrez]
AID - S0378-1135(19)30539-5 [pii]
AID - 10.1016/j.vetmic.2019.06.011 [doi]
PST - ppublish
SO  - Vet Microbiol. 2019 Aug;235:101-109. doi: 10.1016/j.vetmic.2019.06.011. Epub 2019 
      Jun 12.