PMID- 31284128
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 2162-2531 (Print)
IS  - 2162-2531 (Electronic)
IS  - 2162-2531 (Linking)
VI  - 17
DP  - 2019 Sep 6
TI  - miR-892b Inhibits Hypertrophy by Targeting KLF10 in the Chondrogenesis of 
      Mesenchymal Stem Cells.
PG  - 310-322
LID - S2162-2531(19)30162-3 [pii]
LID - 10.1016/j.omtn.2019.05.029 [doi]
AB  - We investigated the functional role of miR-892b as a novel inhibitor of 
      chondrocyte hypertrophy during TGF-beta-mediated chondrogenesis of human mesenchymal 
      stem cells (hMSCs). The expression of miR-892b during TGF-beta-mediated 
      chondrogenesis of hMSCs and the effects of miR-892b overexpression on 
      chondrogenic and hypertrophic marker genes in the chondrogenesis of hMSCs were 
      investigated. Targets of miR-892b were identified and verified by overexpression 
      of synthetic miRNA mimics and luciferase assays. Cross-talk between Kruppel-like 
      factor 10 (KLF10) and Indian hedgehog (Ihh) was investigated using KLF10 
      knockdown (KD). miR-892b enhanced chondrogenic makers and suppressed hypertrophy 
      in hMSC chondrogenesis, mimicking parathyroid hormone-related peptide (PTHrP). 
      KLF10, a transcription factor and miR-892b target, directly regulated Ihh 
      promoter activity. Like miR-892b, KLF10 KD enhanced hMSC chondrogenesis and 
      inhibited hypertrophy. Our findings suggest a key role of miR-892b in targeting 
      the KLF10-Ihh axis as a regulator of hypertrophy in TGF-beta-mediated chondrogenesis 
      of hMSCs and provide a novel strategy for preventing hypertrophy in 
      chondrogenesis from MSCs.
CI  - Copyright (c) 2019 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Lee, Jong Min
AU  - Lee JM
AD  - Research Institute for Integrative Regenerative Biomedical Engineering, Dongguk 
      University, Goyang 10326, Republic of Korea.
FAU - Ko, Ji-Yun
AU  - Ko JY
AD  - Research Institute for Integrative Regenerative Biomedical Engineering, Dongguk 
      University, Goyang 10326, Republic of Korea.
FAU - Kim, Hye Young
AU  - Kim HY
AD  - Research Institute for Integrative Regenerative Biomedical Engineering, Dongguk 
      University, Goyang 10326, Republic of Korea.
FAU - Park, Jeong-Won
AU  - Park JW
AD  - Research Institute for Integrative Regenerative Biomedical Engineering, Dongguk 
      University, Goyang 10326, Republic of Korea.
FAU - Guilak, Farshid
AU  - Guilak F
AD  - Department of Orthopaedic Surgery, Washington University and Shriners Hospitals 
      for Children-St. Louis, St. Louis, MO 63110, USA.
FAU - Im, Gun-Il
AU  - Im GI
AD  - Research Institute for Integrative Regenerative Biomedical Engineering, Dongguk 
      University, Goyang 10326, Republic of Korea. Electronic address: 
      imgunil@hanmail.net.
LA  - eng
PT  - Journal Article
DEP - 20190613
PL  - United States
TA  - Mol Ther Nucleic Acids
JT  - Molecular therapy. Nucleic acids
JID - 101581621
PMC - PMC6612925
OTO - NOTNLM
OT  - KLF10
OT  - chondrogenesis
OT  - hypertrophy
OT  - mesenchymal stem cells
OT  - miR-892b
EDAT- 2019/07/10 06:00
MHDA- 2019/07/10 06:01
PMCR- 2019/06/13
CRDT- 2019/07/09 06:00
PHST- 2018/11/08 00:00 [received]
PHST- 2019/05/16 00:00 [revised]
PHST- 2019/05/31 00:00 [accepted]
PHST- 2019/07/10 06:00 [pubmed]
PHST- 2019/07/10 06:01 [medline]
PHST- 2019/07/09 06:00 [entrez]
PHST- 2019/06/13 00:00 [pmc-release]
AID - S2162-2531(19)30162-3 [pii]
AID - 10.1016/j.omtn.2019.05.029 [doi]
PST - ppublish
SO  - Mol Ther Nucleic Acids. 2019 Sep 6;17:310-322. doi: 10.1016/j.omtn.2019.05.029. 
      Epub 2019 Jun 13.