PMID- 31284659
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 2076-2615 (Print)
IS  - 2076-2615 (Electronic)
IS  - 2076-2615 (Linking)
VI  - 9
IP  - 7
DP  - 2019 Jul 7
TI  - Whole Blood Transcriptome Analysis Reveals Positive Effects of Dried Olive 
      Pomace-Supplemented Diet on Inflammation and Cholesterol in Laying Hens.
LID - 10.3390/ani9070427 [doi]
LID - 427
AB  - Olive pomace (OP) represents one of the by-products of the olive industry and 
      represents an important source of bioactive compounds. This characteristic makes 
      OP a potential feed supplement in livestock nutrition. Thus, in the last years, 
      several studies have been published to evaluate the productive traits following 
      OP supplementation in animal diets; however, relatively little is known from a 
      molecular biology standpoint. Therefore, in this study, we report the 
      RNA-sequencing analysis of laying hens fed with a 10% dried OP (DOP) 
      supplementation. Applying a false discovery rate (FDR) <0.05 and a Log(2)Fc 
      either less than -1.5 or higher than +1.5, we identified 264 differentially 
      regulated genes (DEGs) between the non-supplemented diet control group (CTR) and 
      the DOP group. Using the 264 DEGs to identify enriched biological pathways, we 
      noted that cholesterol biosynthesis showed the highest enrichment followed by 
      several pathways related to immune response and inflammation. As a consequence, 
      when we quantified the cholesterol amount in yolk egg, we found a significant 
      reduction in the DOP vs. the CTR group (p < 0.05). In conclusion, this study 
      shows that DPO affects gene expression in laying hens, which is directly 
      correlated with cholesterol decrease and can potentially ameliorate health status 
      influencing immune response and inflammation.
FAU - Iannaccone, Marco
AU  - Iannaccone M
AUID- ORCID: 0000-0002-2283-6613
AD  - Faculty of Bioscience and Technology for Food, Agriculture, and Environment, 
      University of Teramo, Via R. Balzarini 1, 64100 Teramo, Italy.
FAU - Ianni, Andrea
AU  - Ianni A
AUID- ORCID: 0000-0003-3102-6804
AD  - Faculty of Bioscience and Technology for Food, Agriculture, and Environment, 
      University of Teramo, Via R. Balzarini 1, 64100 Teramo, Italy.
FAU - Ramazzotti, Solange
AU  - Ramazzotti S
AD  - Faculty of Bioscience and Technology for Food, Agriculture, and Environment, 
      University of Teramo, Via R. Balzarini 1, 64100 Teramo, Italy.
FAU - Grotta, Lisa
AU  - Grotta L
AD  - Faculty of Bioscience and Technology for Food, Agriculture, and Environment, 
      University of Teramo, Via R. Balzarini 1, 64100 Teramo, Italy.
FAU - Marone, Elettra
AU  - Marone E
AUID- ORCID: 0000-0003-1343-0387
AD  - Faculty of Bioscience and Technology for Food, Agriculture, and Environment, 
      University of Teramo, Via R. Balzarini 1, 64100 Teramo, Italy.
FAU - Cichelli, Angelo
AU  - Cichelli A
AD  - Department of Medical, Oral and Biotechnological Sciences, G. D.'Annunzio 
      University, Via dei Vestini,31, 66100 Chieti, Italy.
FAU - Martino, Giuseppe
AU  - Martino G
AUID- ORCID: 0000-0002-7878-9318
AD  - Faculty of Bioscience and Technology for Food, Agriculture, and Environment, 
      University of Teramo, Via R. Balzarini 1, 64100 Teramo, Italy. gmartino@unite.it.
LA  - eng
PT  - Journal Article
DEP - 20190707
PL  - Switzerland
TA  - Animals (Basel)
JT  - Animals : an open access journal from MDPI
JID - 101635614
PMC - PMC6680721
OTO - NOTNLM
OT  - RNA-sequencing
OT  - dried olive pomace
OT  - laying hens
COIS- The authors declare that they have no competing interest.
EDAT- 2019/07/10 06:00
MHDA- 2019/07/10 06:01
PMCR- 2019/07/07
CRDT- 2019/07/10 06:00
PHST- 2019/05/22 00:00 [received]
PHST- 2019/06/24 00:00 [revised]
PHST- 2019/07/03 00:00 [accepted]
PHST- 2019/07/10 06:00 [entrez]
PHST- 2019/07/10 06:00 [pubmed]
PHST- 2019/07/10 06:01 [medline]
PHST- 2019/07/07 00:00 [pmc-release]
AID - ani9070427 [pii]
AID - animals-09-00427 [pii]
AID - 10.3390/ani9070427 [doi]
PST - epublish
SO  - Animals (Basel). 2019 Jul 7;9(7):427. doi: 10.3390/ani9070427.