PMID- 31284679
OWN - NLM
STAT- MEDLINE
DCOM- 20200430
LR  - 20200430
IS  - 2218-273X (Electronic)
IS  - 2218-273X (Linking)
VI  - 9
IP  - 7
DP  - 2019 Jul 7
TI  - Roles of Zinc Finger Protein 423 in Proliferation and Invasion of 
      Cholangiocarcinoma through Oxidative Stress.
LID - 10.3390/biom9070263 [doi]
LID - 263
AB  - Zinc finger protein 423 (ZNF423) is a transcriptional factor involved in the 
      development and progression of cancers but has not yet been examined in 
      cholangiocarcinoma (CCA), an oxidative stress-driven cancer of biliary 
      epithelium. In this study, we hypothesized that oxidative stress mediated ZNF423 
      expression regulates its downstream genes resulting in CCA genesis. ZNF423 
      protein expression patterns and 8-oxodG (an oxidative stress marker) formation in 
      CCA tissues were investigated using immunohistochemical analysis. The results 
      showed that ZNF423 was overexpressed in CCA cells compared to normal bile duct 
      cells adjacent of the tumor. Notably, ZNF423 expression was positively correlated 
      with 8-oxodG formation. Moreover, ZNF423 expression in an immortalized 
      cholangiocyte cell line (MMNK1) was increased by hydrogen peroxide-treatment, 
      suggesting that oxidative stress induces ZNF423 expression. To investigate the 
      roles of ZNF423 in CCA progression, ZNF423 mRNA was silenced using specific siRNA 
      in CCA cell lines, KKU-100 and KKU-213. Silencing of ZNF423 significantly 
      inhibits cell proliferation and invasion of both CCA cell lines. Taking all these 
      results together, the present study denoted that ZNF423 is an oxidative 
      stress-responsive gene with an oncogenic property contributing to the regulation 
      of CCA genesis.
FAU - Chaiprasert, Timpika
AU  - Chaiprasert T
AD  - Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen 
      40002, Thailand.
AD  - Cholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen 40002, 
      Thailand.
FAU - Armartmuntree, Napat
AU  - Armartmuntree N
AD  - Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen 
      40002, Thailand.
AD  - Cholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen 40002, 
      Thailand.
FAU - Techasen, Anchalee
AU  - Techasen A
AD  - Cholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen 40002, 
      Thailand.
AD  - Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, 
      Thailand.
FAU - Sakonsinsiri, Chadamas
AU  - Sakonsinsiri C
AUID- ORCID: 0000-0003-0145-8753
AD  - Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen 
      40002, Thailand.
AD  - Cholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen 40002, 
      Thailand.
FAU - Pinlaor, Somchai
AU  - Pinlaor S
AD  - Cholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen 40002, 
      Thailand.
AD  - Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 
      40002, Thailand.
FAU - Ungarreevittaya, Piti
AU  - Ungarreevittaya P
AD  - Cholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen 40002, 
      Thailand.
AD  - Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen 
      40002, Thailand.
FAU - Khuntikeo, Narong
AU  - Khuntikeo N
AD  - Cholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen 40002, 
      Thailand.
AD  - Department of Surgery, Faculty of Medicine, Khon Kaen University, Khon Kaen 
      40002, Thailand.
FAU - Namwat, Nisana
AU  - Namwat N
AD  - Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen 
      40002, Thailand.
AD  - Cholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen 40002, 
      Thailand.
FAU - Thanan, Raynoo
AU  - Thanan R
AUID- ORCID: 0000-0002-9001-1197
AD  - Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen 
      40002, Thailand. raynoo@kku.ac.th.
AD  - Cholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen 40002, 
      Thailand. raynoo@kku.ac.th.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190707
PL  - Switzerland
TA  - Biomolecules
JT  - Biomolecules
JID - 101596414
RN  - 0 (Proteins)
RN  - 0 (ornithine decarboxylase antizyme)
SB  - IM
MH  - Bile Duct Neoplasms/genetics/*metabolism/pathology
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Cholangiocarcinoma/genetics/*metabolism/pathology
MH  - Humans
MH  - *Oxidative Stress
MH  - Proteins/genetics/*metabolism
PMC - PMC6681239
OTO - NOTNLM
OT  - cancer progression
OT  - cholangiocarcinoma
OT  - oxidative stress
OT  - zinc finger protein 423 (ZNF423)
COIS- The authors declare no conflict of interest.
EDAT- 2019/07/10 06:00
MHDA- 2020/05/01 06:00
PMCR- 2019/07/01
CRDT- 2019/07/10 06:00
PHST- 2019/05/16 00:00 [received]
PHST- 2019/06/26 00:00 [revised]
PHST- 2019/07/03 00:00 [accepted]
PHST- 2019/07/10 06:00 [entrez]
PHST- 2019/07/10 06:00 [pubmed]
PHST- 2020/05/01 06:00 [medline]
PHST- 2019/07/01 00:00 [pmc-release]
AID - biom9070263 [pii]
AID - biomolecules-09-00263 [pii]
AID - 10.3390/biom9070263 [doi]
PST - epublish
SO  - Biomolecules. 2019 Jul 7;9(7):263. doi: 10.3390/biom9070263.