PMID- 31286855 OWN - NLM STAT- MEDLINE DCOM- 20191209 LR - 20200225 IS - 1471-2164 (Electronic) IS - 1471-2164 (Linking) VI - 20 IP - 1 DP - 2019 Jul 8 TI - Microarray analysis of infectious bronchitis virus infection of chicken primary dendritic cells. PG - 557 LID - 10.1186/s12864-019-5940-6 [doi] LID - 557 AB - BACKGROUND: Avian infectious bronchitis virus (IBV) is a major respiratory disease-causing agent in birds that leads to significant losses. Dendritic cells (DCs) are specialised cells responsible for sampling antigens and presenting them to T cells, which also play an essential role in recognising and neutralising viruses. Recent studies have suggested that non-coding RNAs may regulate the functional program of DCs. Expression of host non-coding RNAs changes markedly during infectious bronchitis virus infection, but their role in regulating host immune function has not been explored. Here, microarrays of mRNAs, miRNAs, and lncRNAs were globally performed to analyse how avian DCs respond to IBV. RESULTS: First, we found that IBV stimulation did not enhance the maturation ability of avian DCs. Interestingly, inactivated IBV was better able than IBV to induce DC maturation and activate lymphocytes. We identified 1093 up-regulated and 845 down-regulated mRNAs in IBV-infected avian DCs. Gene Ontology analysis suggested that cellular macromolecule and protein location (GO-BP) and transcription factor binding (GO-MF) were abundant in IBV-stimulated avian DCs. Meanwhile, pathway analysis indicated that the oxidative phosphorylation and leukocyte transendothelial migration signalling pathways might be activated in the IBV group. Moreover, alteration of microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) was detected in IBV-stimulated avian DCs. In total, 19 significantly altered (7 up and 12 down) miRNAs and 101 (75 up and 26 down) lncRNAs were identified in the IBV-treated group. Further analysis showed that the actin cytoskeleton and MAPK signal pathway were related to the target genes of IBV-stimulated miRNAs. Finally, our study identified 2 TF-microRNA and 53 TF-microRNA-mRNA interactions involving 1 TF, 2 miRNAs, and 53 mRNAs in IBV-stimulated avian DCs. CONCLUSIONS: Our research suggests a new mechanism to explain why IBV actively blocks innate responses needed for inducing immune gene expression and also provides insight into the pathogenic mechanisms of avian IBV. FAU - Lin, Jian AU - Lin J AD - College of Life Sciences, Nanjing Agricultural University, Wei gang 1, Nanjing, Jiangsu, 210095, People's Republic of China. AD - College of Veterinary medicine, Nanjing Agricultural University, Wei gang 1, Nanjing, Jiangsu, 210095, People's Republic of China. FAU - Wang, Zhisheng AU - Wang Z AD - National Veterinary Product Engineering Research Center, Jiangsu Academy of Agricultural Sciences, Nanjing, China. FAU - Wang, Jialu AU - Wang J AD - College of Veterinary medicine, Nanjing Agricultural University, Wei gang 1, Nanjing, Jiangsu, 210095, People's Republic of China. FAU - Yang, Qian AU - Yang Q AD - College of Life Sciences, Nanjing Agricultural University, Wei gang 1, Nanjing, Jiangsu, 210095, People's Republic of China. zxbyq@njau.edu.cn. AD - College of Veterinary medicine, Nanjing Agricultural University, Wei gang 1, Nanjing, Jiangsu, 210095, People's Republic of China. zxbyq@njau.edu.cn. LA - eng GR - 2017YFD500706/National Key Research and Development Program of China/ GR - No. 31702197/National Natural Science Grant of P. R. China/ GR - CX (13)5035/Independent Innovation of Agricultural Sciences Program of Jiangsu Province/ GR - PAPD/A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions/ GR - JCQY201906/the Fundamental Research Funds for the Central Universities/ GR - KJQN2018034/the Fundamental Research Funds for the Central Universities/ PT - Journal Article DEP - 20190708 PL - England TA - BMC Genomics JT - BMC genomics JID - 100965258 RN - 0 (MicroRNAs) RN - 0 (RNA, Long Noncoding) RN - 0 (RNA, Messenger) SB - IM MH - Animals MH - Bone Marrow Cells/cytology MH - *Computational Biology MH - Coronavirus Infections/*genetics/*immunology MH - Dendritic Cells/cytology/*metabolism MH - Gene Expression Profiling MH - Infectious bronchitis virus/*physiology MH - Mice MH - MicroRNAs/genetics MH - *Oligonucleotide Array Sequence Analysis MH - RNA, Long Noncoding/genetics MH - RNA, Messenger/genetics PMC - PMC6615177 OTO - NOTNLM OT - Dendritic cells OT - Infectious bronchitis virus OT - lncRNA OT - mRNA OT - microRNA COIS- The authors of this editorial have no competing interest to declare. The authors have no other relevant affiliations or financial involvement in any organization or entity with a financial interest in or financial competing with the subject matter or materials discussed in the manuscript apart from those disclosed. EDAT- 2019/07/10 06:00 MHDA- 2019/12/18 06:00 PMCR- 2019/07/08 CRDT- 2019/07/10 06:00 PHST- 2018/07/26 00:00 [received] PHST- 2019/06/26 00:00 [accepted] PHST- 2019/07/10 06:00 [entrez] PHST- 2019/07/10 06:00 [pubmed] PHST- 2019/12/18 06:00 [medline] PHST- 2019/07/08 00:00 [pmc-release] AID - 10.1186/s12864-019-5940-6 [pii] AID - 5940 [pii] AID - 10.1186/s12864-019-5940-6 [doi] PST - epublish SO - BMC Genomics. 2019 Jul 8;20(1):557. doi: 10.1186/s12864-019-5940-6.