PMID- 31287215
OWN - NLM
STAT- MEDLINE
DCOM- 20200625
LR  - 20200625
IS  - 1615-9861 (Electronic)
IS  - 1615-9853 (Linking)
VI  - 19
IP  - 21-22
DP  - 2019 Nov
TI  - Mass Spectrometric Analysis Identifies AIMP1 and LTA4H as FSCN1-Binding Proteins 
      in Laryngeal Squamous Cell Carcinoma.
PG  - e1900059
LID - 10.1002/pmic.201900059 [doi]
AB  - Dysregulation of fascin actin-bundling protein 1 (FSCN1) enhances cell 
      proliferation, invasion, and motility in laryngeal squamous cell carcinoma 
      (LSCC), while the mechanism remains unclear. Here, co-immunoprecipitation and 
      mass spectrometry is utilized to identify potential FSCN1-binding proteins. 
      Functional annotation of FSCN1-binding proteins are performed by Gene Ontology 
      and Kyoto Encyclopedia of Genes and Genomes pathway analysis. Furthermore, the 
      protein-protein interaction network of FSNC1-binding proteins is constructed and 
      the interactions between FSCN1 and novel identified interacting proteins AIMP1 
      and LTA4H are validated. Moreover, the expression and functional role of AIMP1 
      and LTA4H in LSCC are investigated. A total of 123 proteins are identified as 
      potential FSCN1-binding proteins, and functional annotation shows that 
      FSCN1-binding proteins are significantly enriched in carcinogenic processes, such 
      as filopodium assembly-regulation and GTPase activity. Co-IP/western blotting and 
      immunofluorescence confirm that AIMP1 and LTA4H bind and colocalize with FSCN1. 
      Furthermore, both AIMP1 and LTA4H are upregulated in LSCC tissues, and knockdown 
      of AIMP1 or LTA4H inhibits LSCC cell proliferation, migration, and invasion. 
      Collectively, the identification of FSCN1-binding partners enhances understanding 
      of the mechanism of FSCN1-mediated malignant phenotypes, and these findings 
      indicate that FSCN1 binds to AIMP1 and LTA4H might promote the progression of 
      LSCC.
CI  - (c) 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Gao, Wei
AU  - Gao W
AUID- ORCID: 0000-0001-7836-2851
AD  - Shanxi Key Laboratory of Otorhinolaryngology, Head and Neck Cancer, Shanxi 
      Medical University, Taiyuan, 030001, Shanxi, China.
AD  - Department of Otolaryngology Head & Neck Surgery, The First Hospital of Shanxi 
      Medical University, Taiyuan, 030001, Shanxi, China.
AD  - Otolaryngology Head & Neck Surgery Research Institute, Shanxi Medical University, 
      Taiyuan, 030001, Shanxi, China.
AD  - The Key Scientific and Technological Innovation Platform for Precision Diagnosis 
      and Treatment of Head and Neck Cancer, Taiyuan, 030001, Shanxi, China.
FAU - An, Changming
AU  - An C
AD  - Department of Head and Neck Surgery Cancer Hospital, National Cancer Center, 
      Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 
      100021, China.
FAU - Xue, Xuting
AU  - Xue X
AD  - Shanxi Key Laboratory of Otorhinolaryngology, Head and Neck Cancer, Shanxi 
      Medical University, Taiyuan, 030001, Shanxi, China.
AD  - Department of Otolaryngology Head & Neck Surgery, The First Hospital of Shanxi 
      Medical University, Taiyuan, 030001, Shanxi, China.
AD  - Otolaryngology Head & Neck Surgery Research Institute, Shanxi Medical University, 
      Taiyuan, 030001, Shanxi, China.
AD  - The Key Scientific and Technological Innovation Platform for Precision Diagnosis 
      and Treatment of Head and Neck Cancer, Taiyuan, 030001, Shanxi, China.
FAU - Zheng, Xiwang
AU  - Zheng X
AD  - Shanxi Key Laboratory of Otorhinolaryngology, Head and Neck Cancer, Shanxi 
      Medical University, Taiyuan, 030001, Shanxi, China.
AD  - Department of Otolaryngology Head & Neck Surgery, The First Hospital of Shanxi 
      Medical University, Taiyuan, 030001, Shanxi, China.
AD  - Otolaryngology Head & Neck Surgery Research Institute, Shanxi Medical University, 
      Taiyuan, 030001, Shanxi, China.
AD  - The Key Scientific and Technological Innovation Platform for Precision Diagnosis 
      and Treatment of Head and Neck Cancer, Taiyuan, 030001, Shanxi, China.
FAU - Niu, Min
AU  - Niu M
AD  - Shanxi Key Laboratory of Otorhinolaryngology, Head and Neck Cancer, Shanxi 
      Medical University, Taiyuan, 030001, Shanxi, China.
AD  - Department of Otolaryngology Head & Neck Surgery, The First Hospital of Shanxi 
      Medical University, Taiyuan, 030001, Shanxi, China.
AD  - Otolaryngology Head & Neck Surgery Research Institute, Shanxi Medical University, 
      Taiyuan, 030001, Shanxi, China.
AD  - The Key Scientific and Technological Innovation Platform for Precision Diagnosis 
      and Treatment of Head and Neck Cancer, Taiyuan, 030001, Shanxi, China.
FAU - Zhang, Yuliang
AU  - Zhang Y
AD  - Shanxi Key Laboratory of Otorhinolaryngology, Head and Neck Cancer, Shanxi 
      Medical University, Taiyuan, 030001, Shanxi, China.
AD  - Department of Otolaryngology Head & Neck Surgery, The First Hospital of Shanxi 
      Medical University, Taiyuan, 030001, Shanxi, China.
AD  - Otolaryngology Head & Neck Surgery Research Institute, Shanxi Medical University, 
      Taiyuan, 030001, Shanxi, China.
AD  - The Key Scientific and Technological Innovation Platform for Precision Diagnosis 
      and Treatment of Head and Neck Cancer, Taiyuan, 030001, Shanxi, China.
FAU - Liu, Hongliang
AU  - Liu H
AD  - Shanxi Key Laboratory of Otorhinolaryngology, Head and Neck Cancer, Shanxi 
      Medical University, Taiyuan, 030001, Shanxi, China.
AD  - Department of Otolaryngology Head & Neck Surgery, The First Hospital of Shanxi 
      Medical University, Taiyuan, 030001, Shanxi, China.
AD  - Otolaryngology Head & Neck Surgery Research Institute, Shanxi Medical University, 
      Taiyuan, 030001, Shanxi, China.
AD  - The Key Scientific and Technological Innovation Platform for Precision Diagnosis 
      and Treatment of Head and Neck Cancer, Taiyuan, 030001, Shanxi, China.
FAU - Zhang, Chunming
AU  - Zhang C
AD  - Shanxi Key Laboratory of Otorhinolaryngology, Head and Neck Cancer, Shanxi 
      Medical University, Taiyuan, 030001, Shanxi, China.
AD  - Department of Otolaryngology Head & Neck Surgery, The First Hospital of Shanxi 
      Medical University, Taiyuan, 030001, Shanxi, China.
AD  - Otolaryngology Head & Neck Surgery Research Institute, Shanxi Medical University, 
      Taiyuan, 030001, Shanxi, China.
AD  - The Key Scientific and Technological Innovation Platform for Precision Diagnosis 
      and Treatment of Head and Neck Cancer, Taiyuan, 030001, Shanxi, China.
FAU - Lu, Yan
AU  - Lu Y
AD  - Department of Otolaryngology Head & Neck Surgery, The First Hospital, Jinzhou 
      Medical University, Jinzhou, 121001, Liaoning, China.
FAU - Cui, Jiajia
AU  - Cui J
AD  - Shanxi Key Laboratory of Otorhinolaryngology, Head and Neck Cancer, Shanxi 
      Medical University, Taiyuan, 030001, Shanxi, China.
AD  - Department of Otolaryngology Head & Neck Surgery, The First Hospital of Shanxi 
      Medical University, Taiyuan, 030001, Shanxi, China.
AD  - Otolaryngology Head & Neck Surgery Research Institute, Shanxi Medical University, 
      Taiyuan, 030001, Shanxi, China.
AD  - The Key Scientific and Technological Innovation Platform for Precision Diagnosis 
      and Treatment of Head and Neck Cancer, Taiyuan, 030001, Shanxi, China.
FAU - Zhao, Qinli
AU  - Zhao Q
AD  - Shanxi Key Laboratory of Otorhinolaryngology, Head and Neck Cancer, Shanxi 
      Medical University, Taiyuan, 030001, Shanxi, China.
AD  - Department of Otolaryngology Head & Neck Surgery, The First Hospital of Shanxi 
      Medical University, Taiyuan, 030001, Shanxi, China.
FAU - Wen, Shuxin
AU  - Wen S
AD  - Shanxi Key Laboratory of Otorhinolaryngology, Head and Neck Cancer, Shanxi 
      Medical University, Taiyuan, 030001, Shanxi, China.
AD  - Department of Otolaryngology Head & Neck Surgery, The First Hospital of Shanxi 
      Medical University, Taiyuan, 030001, Shanxi, China.
AD  - Otolaryngology Head & Neck Surgery Research Institute, Shanxi Medical University, 
      Taiyuan, 030001, Shanxi, China.
AD  - The Key Scientific and Technological Innovation Platform for Precision Diagnosis 
      and Treatment of Head and Neck Cancer, Taiyuan, 030001, Shanxi, China.
FAU - Thorne, Rick F
AU  - Thorne RF
AD  - Translational Research Institute, Henan Provincial People's Hospital, School of 
      Medicine, Henan University, Zhengzhou, 450053, Henan, China.
AD  - School of Environmental and Life Sciences, The University of Newcastle, 
      Callaghan, NSW, 2308, Australia.
FAU - Zhang, Xudong
AU  - Zhang X
AD  - School of Biomedical Sciences and Pharmacy, The University of Newcastle, 
      Callaghan, NSW, 2308, Australia.
FAU - Wu, Yongyan
AU  - Wu Y
AUID- ORCID: 0000-0003-1669-3860
AD  - Shanxi Key Laboratory of Otorhinolaryngology, Head and Neck Cancer, Shanxi 
      Medical University, Taiyuan, 030001, Shanxi, China.
AD  - Department of Otolaryngology Head & Neck Surgery, The First Hospital of Shanxi 
      Medical University, Taiyuan, 030001, Shanxi, China.
AD  - Otolaryngology Head & Neck Surgery Research Institute, Shanxi Medical University, 
      Taiyuan, 030001, Shanxi, China.
AD  - The Key Scientific and Technological Innovation Platform for Precision Diagnosis 
      and Treatment of Head and Neck Cancer, Taiyuan, 030001, Shanxi, China.
FAU - Wang, Binquan
AU  - Wang B
AUID- ORCID: 0000-0002-3686-0219
AD  - Shanxi Key Laboratory of Otorhinolaryngology, Head and Neck Cancer, Shanxi 
      Medical University, Taiyuan, 030001, Shanxi, China.
AD  - Department of Otolaryngology Head & Neck Surgery, The First Hospital of Shanxi 
      Medical University, Taiyuan, 030001, Shanxi, China.
AD  - Otolaryngology Head & Neck Surgery Research Institute, Shanxi Medical University, 
      Taiyuan, 030001, Shanxi, China.
AD  - The Key Scientific and Technological Innovation Platform for Precision Diagnosis 
      and Treatment of Head and Neck Cancer, Taiyuan, 030001, Shanxi, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190726
PL  - Germany
TA  - Proteomics
JT  - Proteomics
JID - 101092707
RN  - 0 (Carrier Proteins)
RN  - 0 (Cytokines)
RN  - 0 (FSCN1 protein, human)
RN  - 0 (Microfilament Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (small inducible cytokine subfamily E, member 1)
RN  - EC 3.3.2.- (Epoxide Hydrolases)
RN  - V38765PUZ6 (leukotriene A4 hydrolase)
SB  - IM
MH  - Carcinoma, Squamous Cell/*genetics/pathology
MH  - Carrier Proteins/*genetics
MH  - Cell Movement/genetics
MH  - Cell Proliferation/genetics
MH  - Cytokines/*genetics
MH  - Epoxide Hydrolases/*genetics
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics/pathology
MH  - Mass Spectrometry
MH  - Microfilament Proteins/*genetics
MH  - Neoplasm Invasiveness/genetics/pathology
MH  - Neoplasm Proteins/*genetics
MH  - Protein Binding/genetics
MH  - Protein Interaction Maps/genetics
MH  - RNA-Binding Proteins/*genetics
OTO - NOTNLM
OT  - AIMP1
OT  - FSCN1
OT  - LTA4H
OT  - laryngeal squamous cell carcinoma
OT  - protein-protein interactions
EDAT- 2019/07/10 06:00
MHDA- 2020/06/26 06:00
CRDT- 2019/07/10 06:00
PHST- 2019/02/05 00:00 [received]
PHST- 2019/06/29 00:00 [revised]
PHST- 2019/07/10 06:00 [pubmed]
PHST- 2020/06/26 06:00 [medline]
PHST- 2019/07/10 06:00 [entrez]
AID - 10.1002/pmic.201900059 [doi]
PST - ppublish
SO  - Proteomics. 2019 Nov;19(21-22):e1900059. doi: 10.1002/pmic.201900059. Epub 2019 
      Jul 26.