PMID- 31287373
OWN - NLM
STAT- MEDLINE
DCOM- 20200131
LR  - 20200131
IS  - 1362-3095 (Electronic)
IS  - 0955-3002 (Linking)
VI  - 95
IP  - 11
DP  - 2019 Nov
TI  - Low-dose ionizing radiation attenuates mast cell migration through suppression of 
      monocyte chemoattractant protein-1 (MCP-1) expression by Nr4a2.
PG  - 1498-1506
LID - 10.1080/09553002.2019.1642535 [doi]
AB  - Purpose: The aim of this study was to investigate whether low-dose ionizing 
      radiation attenuates mast cell migration by modulating migration-associated 
      signaling pathways and the expression of chemotactic cytokines.Materials and 
      methods: IgE-sensitized RBL-2H3 mast cells were exposed with ionizing radiation 
      at 0.01, 0.05, 0.1, or 0.5 Gy using a (137)Cs gamma-irradiator and stimulated with 
      2,4-dinitrophenol-human serum albumin. Cell migration was determined using a 
      transwell assay system, F-actin distribution using Alex Fluor 488-conjugated 
      phalloidin, expression of various signaling proteins by Western blotting, mRNA 
      expression by RT-PCR.Results: Low-dose ionizing radiation significantly 
      suppressed mast cell migration induced by IgE-mediated mast cell activation. 
      Furthermore, low-dose ionizing radiation altered cell morphology, as reflected by 
      changes in F-actin distribution, and inhibited the activation of PI3K, Btk, Rac1, 
      and Cdc42. These effects were mediated by Nr4a2, an immune-modulating factor. 
      Knockdown of Nr4a2 reduced mast cell migration, inhibited the PI3K and Btk 
      signaling pathways, and reduced expression of the chemotactic cytokine monocyte 
      chemoattractant protein-1 (MCP-1). We further demonstrated that direct blockade 
      of MCP-1 using neutralizing antibodies inhibits mast cell migration.Conclusion: 
      Low-dose ionizing radiation inhibits mast cell migration through the regulation 
      production of MCP-1 by Nr4a2 in the activated mast cell system.
FAU - Song, Chin-Hee
AU  - Song CH
AD  - Low-dose Radiation Research Team, Radiation Health Institute, Korea Hydro & 
      Nuclear Power Co., Ltd, Seoul, Republic of Korea.
FAU - Joo, Hae Mi
AU  - Joo HM
AD  - Low-dose Radiation Research Team, Radiation Health Institute, Korea Hydro & 
      Nuclear Power Co., Ltd, Seoul, Republic of Korea.
FAU - Han, So Hyun
AU  - Han SH
AD  - Low-dose Radiation Research Team, Radiation Health Institute, Korea Hydro & 
      Nuclear Power Co., Ltd, Seoul, Republic of Korea.
FAU - Kim, Jeong-In
AU  - Kim JI
AD  - Radiation Emergency Medical Team, Radiation Health Institute, Korea Hydro & 
      Nuclear Power Co., Ltd, Seoul, Republic of Korea.
FAU - Nam, Seon Young
AU  - Nam SY
AD  - Low-dose Radiation Research Team, Radiation Health Institute, Korea Hydro & 
      Nuclear Power Co., Ltd, Seoul, Republic of Korea.
FAU - Kim, Ji Young
AU  - Kim JY
AD  - Low-dose Radiation Research Team, Radiation Health Institute, Korea Hydro & 
      Nuclear Power Co., Ltd, Seoul, Republic of Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190809
PL  - England
TA  - Int J Radiat Biol
JT  - International journal of radiation biology
JID - 8809243
RN  - 0 (Actins)
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Cesium Radioisotopes)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 0 (Nr4a2 protein, rat)
RN  - 0 (Nuclear Receptor Subfamily 4, Group A, Member 2)
RN  - 17466-45-4 (Phalloidine)
RN  - 4T2E65IAR7 (Cesium-137)
SB  - IM
MH  - Actins/metabolism
MH  - Animals
MH  - Cell Line, Tumor
MH  - Cell Movement/*radiation effects
MH  - Cesium Radioisotopes
MH  - Chemokine CCL2/*metabolism
MH  - Chemotaxis
MH  - Cytokines/metabolism
MH  - Gamma Rays
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation
MH  - Mast Cells/cytology/*radiation effects
MH  - Nuclear Receptor Subfamily 4, Group A, Member 2/*metabolism
MH  - Phalloidine/metabolism
MH  - *RNA Interference
MH  - Rats
MH  - Signal Transduction
OTO - NOTNLM
OT  - Low-dose ionizing radiation
OT  - MCP-1
OT  - Nr4a2
OT  - chemotactic cytokine
OT  - mast cell migration
OT  - transcription factor
EDAT- 2019/07/10 06:00
MHDA- 2020/02/01 06:00
CRDT- 2019/07/10 06:00
PHST- 2019/07/10 06:00 [pubmed]
PHST- 2020/02/01 06:00 [medline]
PHST- 2019/07/10 06:00 [entrez]
AID - 10.1080/09553002.2019.1642535 [doi]
PST - ppublish
SO  - Int J Radiat Biol. 2019 Nov;95(11):1498-1506. doi: 10.1080/09553002.2019.1642535. 
      Epub 2019 Aug 9.