PMID- 31287961
OWN - NLM
STAT- MEDLINE
DCOM- 20200601
LR  - 20200601
IS  - 1530-6860 (Electronic)
IS  - 0892-6638 (Linking)
VI  - 33
IP  - 10
DP  - 2019 Oct
TI  - Pdlim4 is essential for CCR7-JNK-mediated dendritic cell migration and 
      F-actin-related dendrite formation.
PG  - 11035-11044
LID - 10.1096/fj.201901031 [doi]
AB  - Dendritic cells (DCs) are the most potent professional antigen (Ag)-presenting 
      cells and inducers of T cell-mediated immunity. A previous microarray analysis 
      identified PDZ and LIM domain protein 4 (Pdlim4) as a candidate marker for DC 
      maturation. The aim of this study was to investigate whether Pdlim4 influences DC 
      migration and maturation. Mouse bone marrow-derived DCs were transduced 
      lentivirally with Pdlim4 short hairpin RNA and examined by confocal microscopy, 
      flow cytometry, ELISA, and Western blotting. Pdlim4 was highly induced in 
      LPS-stimulated mature DCs (mDCs). Pdlim4-knockdown mDCs showed reduced expression 
      of molecules associated with Ag presentation and T-cell costimulation, reduced 
      cytokine production, and functional defects in their ability to activate T cells. 
      Moreover, Pdlim4 was necessary for mDC migration via C-C chemokine receptor type 
      7 (CCR7)-JNK in in vitro Transwell assays. The importance of Pdlim4 in DC 
      migration was confirmed with an in vivo migration model in which C57BL/6 mice 
      were injected with fluorescently labeled DCs in the footpad and migration to the 
      popliteal lymph nodes was assessed by flow cytometry. Moreover, dendrite 
      formation in mDCs was remarkably attenuated under Pdlim4 knockdown. Taken 
      together, these results demonstrate that Pdlim4 is necessary for DC migration via 
      CCR7-JNK, dendrite formation, and subsequent development of functional T-cell 
      responses.-Yoo, J.-Y., Jung, N.-C., Lee, J.-H., Choi, S.-Y., Choi, H.-J., Park, 
      S.-Y., Jang, J.-S., Byun, S.-H., Hwang, S.-U., Noh, K.-E., Park, Y., Lee, J., 
      Song, J.-Y., Seo, H. G., Lee, H. S., Lim, D.-S. Pdlim4 is essential for 
      CCR7-JNK-mediated dendritic cell migration and F-actin-related dendrite 
      formation.
FAU - Yoo, Ji-Young
AU  - Yoo JY
AD  - Department of Biotechnology, CHA University, Seongnam, South Korea.
FAU - Jung, Nam-Chul
AU  - Jung NC
AD  - Department of Biotechnology, CHA University, Seongnam, South Korea.
AD  - Pharos Vaccine, Seongnam, South Korea.
FAU - Lee, Jun-Ho
AU  - Lee JH
AD  - Pharos Vaccine, Seongnam, South Korea.
FAU - Choi, So-Yeon
AU  - Choi SY
AD  - Department of Biotechnology, CHA University, Seongnam, South Korea.
FAU - Choi, Hyun-Ji
AU  - Choi HJ
AD  - JW CreaGene Company, Seongnam, South Korea.
FAU - Park, Soo-Yeoun
AU  - Park SY
AD  - Aprogen, Seongnam, South Korea.
FAU - Jang, Ji-Su
AU  - Jang JS
AD  - Department of Biotechnology, CHA University, Seongnam, South Korea.
FAU - Byun, Sei-Hee
AU  - Byun SH
AD  - Aprogen, Seongnam, South Korea.
FAU - Hwang, Sung-Uk
AU  - Hwang SU
AD  - Pharos Vaccine, Seongnam, South Korea.
FAU - Noh, Kyung-Eun
AU  - Noh KE
AD  - Department of Biotechnology, CHA University, Seongnam, South Korea.
FAU - Park, Yunok
AU  - Park Y
AD  - Department of Biotechnology, CHA University, Seongnam, South Korea.
FAU - Lee, Jongwon
AU  - Lee J
AD  - Department of Biotechnology, CHA University, Seongnam, South Korea.
FAU - Song, Jie-Young
AU  - Song JY
AD  - Department of Radiation Cancer Sciences, Korea Institute of Radiological and 
      Medical Sciences, Seoul, South Korea.
FAU - Seo, Han Geuk
AU  - Seo HG
AD  - Department of Food Science and Biotechnology of Animal Resources, Sanghuh College 
      of Life Sciences, Konkuk University, Seoul, South Korea.
FAU - Lee, Hyun Soo
AU  - Lee HS
AD  - Pharos Vaccine, Seongnam, South Korea.
FAU - Lim, Dae-Seog
AU  - Lim DS
AD  - Department of Biotechnology, CHA University, Seongnam, South Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190709
PL  - United States
TA  - FASEB J
JT  - FASEB journal : official publication of the Federation of American Societies for 
      Experimental Biology
JID - 8804484
RN  - 0 (Actins)
RN  - 0 (Ccr7 protein, mouse)
RN  - 0 (LIM Domain Proteins)
RN  - 0 (Microfilament Proteins)
RN  - 0 (Pdlim4 protein, mouse)
RN  - 0 (Receptors, CCR7)
RN  - EC 2.7.12.2 (MAP Kinase Kinase 4)
SB  - IM
MH  - Actins/metabolism
MH  - Animals
MH  - *Cell Movement
MH  - Cells, Cultured
MH  - Dendritic Cells/immunology/*metabolism/physiology
MH  - LIM Domain Proteins/genetics/*metabolism
MH  - Lymphocyte Activation
MH  - MAP Kinase Kinase 4/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microfilament Proteins/genetics/*metabolism
MH  - Receptors, CCR7/metabolism
OTO - NOTNLM
OT  - DC marker
OT  - DC maturation
OT  - DCs
EDAT- 2019/07/10 06:00
MHDA- 2020/06/02 06:00
CRDT- 2019/07/10 06:00
PHST- 2019/07/10 06:00 [pubmed]
PHST- 2020/06/02 06:00 [medline]
PHST- 2019/07/10 06:00 [entrez]
AID - 10.1096/fj.201901031 [doi]
PST - ppublish
SO  - FASEB J. 2019 Oct;33(10):11035-11044. doi: 10.1096/fj.201901031. Epub 2019 Jul 9.