PMID- 31288972
OWN - NLM
STAT- MEDLINE
DCOM- 20210715
LR  - 20210715
IS  - 1995-9133 (Electronic)
IS  - 1684-1182 (Linking)
VI  - 53
IP  - 4
DP  - 2020 Aug
TI  - Characterization of Salmonella resistance to bile during biofilm formation.
PG  - 518-524
LID - S1684-1182(18)30398-0 [pii]
LID - 10.1016/j.jmii.2019.06.003 [doi]
AB  - BACKGROUND: Non-typhoid Salmonella infection may present as acute gastroenteritis 
      or chronic infection, primarily in the bile-rich gallbladder. Biofilm formation 
      is a mechanism of bile resistance in Salmonella. Our aim was to determine how 
      Salmonella utilizes bile as a signal, and to study the relevance of the 
      interaction between the PhoP-PhoQ two-component system and cyclic diguanosine 
      monophosphate (c-di-GMP) signaling to biofilm formation. METHODS: Two-dimensional 
      (2-D) gel electrophoresis was used to identify genes required for Salmonella 
      biofilm formation in bile. Quantitative real-time PCR (qRT-PCR) was used to 
      clarify the role of the PhoP-PhoQ two-component system and its interaction with 
      genes involved in the c-di-GMP network during biofilm formation. RESULTS: Our 
      result revealed that Salmonella mutants with incomplete outer membrane ( big up tri, openompA), 
      defective flagella ( big up tri, openflgE), or incomplete PhoP-PhoQ two-component system ( big up tri, openphoP), 
      were unable to develop complete biofilms in the presence of bile. Moreover, 
      PhoP-PhoQ two-component system-related Salmonella mutants ( big up tri, openphoP,  big up tri, openphoQ, 
       big up tri, openphoP big up tri, openphoQ) had lower expression of c-di-GMP related genes (csgD, adrA) than the 
      wild-type Salmonella strain had in the bile environment. CONCLUSION: Salmonella 
      may sense and respond to bile through the PhoP-PhoQ two-component system during 
      biofilm formation. Furthermore, the PhoP-PhoQ two-component system might activate 
      regulators of the c-di-GMP signaling network.
CI  - Copyright (c) 2019. Published by Elsevier B.V.
FAU - Tsai, Ming-Han
AU  - Tsai MH
AD  - Department of Pediatrics, Chang Gung Memorial Hospital, Keelung Branch, Keelung, 
      Taiwan; Chang Gung University College of Medicine, Taoyuan, Taiwan; Molecular 
      Infectious Disease Research Center, Chang Gung Memorial Hospital, Taoyuan, 
      Taiwan.
FAU - Liang, Yi-Hua
AU  - Liang YH
AD  - Molecular Infectious Disease Research Center, Chang Gung Memorial Hospital, 
      Taoyuan, Taiwan.
FAU - Chen, Chyi-Liang
AU  - Chen CL
AD  - Chang Gung University College of Medicine, Taoyuan, Taiwan; Molecular Infectious 
      Disease Research Center, Chang Gung Memorial Hospital, Taoyuan, Taiwan.
FAU - Chiu, Cheng-Hsun
AU  - Chiu CH
AD  - Chang Gung University College of Medicine, Taoyuan, Taiwan; Molecular Infectious 
      Disease Research Center, Chang Gung Memorial Hospital, Taoyuan, Taiwan; Division 
      of Pediatric Infectious Diseases, Department of Pediatrics, Chang Gung Children's 
      Hospital, Taoyuan, Taiwan. Electronic address: chchiu@adm.cgmh.org.tw.
LA  - eng
PT  - Journal Article
DEP - 20190625
PL  - England
TA  - J Microbiol Immunol Infect
JT  - Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi
JID - 100956211
RN  - 0 (Bacterial Proteins)
RN  - 125360-99-8 (PhoP protein, Bacteria)
RN  - 61093-23-0 (bis(3',5')-cyclic diguanylic acid)
RN  - H2D2X058MU (Cyclic GMP)
SB  - IM
MH  - Bacterial Proteins/genetics/metabolism
MH  - Bile/*metabolism
MH  - Biofilms/*growth & development
MH  - Cyclic GMP/analogs & derivatives/metabolism
MH  - Humans
MH  - Salmonella Infections/microbiology
MH  - Salmonella typhimurium/*genetics/*metabolism
MH  - *Signal Transduction
OTO - NOTNLM
OT  - Bile
OT  - Biofilm
OT  - Salmonella
EDAT- 2019/07/11 06:00
MHDA- 2021/07/16 06:00
CRDT- 2019/07/11 06:00
PHST- 2018/09/14 00:00 [received]
PHST- 2019/05/05 00:00 [revised]
PHST- 2019/06/06 00:00 [accepted]
PHST- 2019/07/11 06:00 [pubmed]
PHST- 2021/07/16 06:00 [medline]
PHST- 2019/07/11 06:00 [entrez]
AID - S1684-1182(18)30398-0 [pii]
AID - 10.1016/j.jmii.2019.06.003 [doi]
PST - ppublish
SO  - J Microbiol Immunol Infect. 2020 Aug;53(4):518-524. doi: 
      10.1016/j.jmii.2019.06.003. Epub 2019 Jun 25.