PMID- 31289564
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 18
IP  - 1
DP  - 2019 Jul
TI  - Enhanced antitumor effect of cytotoxic T lymphocytes induced by dendritic cells 
      pulsed with colorectal cancer cell lysate expressing alpha-Gal epitopes.
PG  - 864-871
LID - 10.3892/ol.2019.10376 [doi]
AB  - Colorectal cancer (CRC) is one of the most common types of gastrointestinal 
      malignancy. Traditional therapeutic options for CRC exhibit a limited effect. 
      Adoptive cellular therapy has emerged as a new treatment strategy for CRC. 
      Dendritic cells (DCs) are potent antigen-presenting cells. Specific cytotoxic T 
      lymphocytes (CTLs) activated by DCs pulsed with tumor lysate have been reported 
      to be a safe and promising treatment approach for CRC. However, the antitumor 
      effect of specific CTLs remains limited. The low immunogenicity of 
      tumor-associated antigens (TAAs) is the main reason for this limited therapeutic 
      effect. In the present study, alpha-gal epitopes were synthesized on the CRC cell 
      line SW620 to increase the immunogenicity of TAAs. DCs were pulsed with 
      alpha-gal-expressing tumor lysate and CTLs were activated by these DCs. The 
      cytotoxicity of CTLs was measured in vitro. The results demonstrated that DCs 
      pulsed with alpha-gal-expressing tumor lysate can increase the frequency of 
      CD3(+)CD8(+) CTLs and natural killer T cells, increase the level of tumor 
      necrosis factor-alpha produced by CTLs and enhance the cytotoxicity of CTLs against 
      tumor cells. Therefore, this novel approach may be an effective treatment 
      strategy for patients with CRC.
FAU - Xing, Xiaowei
AU  - Xing X
AD  - Department of General Surgery, Chinese People's Liberation Army General Hospital, 
      Beijing 100853, P.R. China.
FAU - Zou, Zhenyu
AU  - Zou Z
AD  - Department of Hernia and Abdominal Wall Surgery, Beijing Chao-Yang Hospital, 
      Capital Medical University, Beijing 100853, P.R. China.
FAU - He, Changzheng
AU  - He C
AD  - Department of General Surgery, Chinese People's Liberation Army General Hospital, 
      Beijing 100853, P.R. China.
FAU - Hu, Zilong
AU  - Hu Z
AD  - Department of General Surgery, Chinese People's Liberation Army General Hospital, 
      Beijing 100853, P.R. China.
FAU - Liang, Kai
AU  - Liang K
AD  - Department of General Surgery, Chinese People's Liberation Army General Hospital, 
      Beijing 100853, P.R. China.
FAU - Liang, Wentao
AU  - Liang W
AD  - Department of General Surgery, Chinese People's Liberation Army General Hospital, 
      Beijing 100853, P.R. China.
FAU - Wang, Yufeng
AU  - Wang Y
AD  - Department of Patient Admission Management, Chinese People's Liberation Army 
      General Hospital, Beijing 100853, P.R. China.
FAU - Du, Xiaohui
AU  - Du X
AD  - Department of General Surgery, Chinese People's Liberation Army General Hospital, 
      Beijing 100853, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20190520
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC6546984
OTO - NOTNLM
OT  - CRC
OT  - DC
OT  - TAA
OT  - cytotoxic T cell
OT  - immunogenicity
OT  - alpha-gal epitopes
EDAT- 2019/07/11 06:00
MHDA- 2019/07/11 06:01
PMCR- 2019/05/20
CRDT- 2019/07/11 06:00
PHST- 2018/06/01 00:00 [received]
PHST- 2019/04/24 00:00 [accepted]
PHST- 2019/07/11 06:00 [entrez]
PHST- 2019/07/11 06:00 [pubmed]
PHST- 2019/07/11 06:01 [medline]
PHST- 2019/05/20 00:00 [pmc-release]
AID - OL-0-0-10376 [pii]
AID - 10.3892/ol.2019.10376 [doi]
PST - ppublish
SO  - Oncol Lett. 2019 Jul;18(1):864-871. doi: 10.3892/ol.2019.10376. Epub 2019 May 20.