PMID- 31293985
OWN - NLM
STAT- MEDLINE
DCOM- 20200218
LR  - 20200309
IS  - 2235-2988 (Electronic)
IS  - 2235-2988 (Linking)
VI  - 9
DP  - 2019
TI  - Treponema pallidum Induces the Secretion of HDVSMC Inflammatory Cytokines to 
      Promote the Migration and Adhesion of THP-1 Cells.
PG  - 220
LID - 10.3389/fcimb.2019.00220 [doi]
LID - 220
AB  - The pathological features of syphilis, a disease caused by Treponema pallidum (T. 
      pallidum), are characterized by vascular involvement with endarteritis and 
      periarteritis. Little is known about the interactions of infiltrating immunocytes 
      with human dermal vascular smooth muscle cells (HDVSMCs) in arterioles during the 
      immunopathogenesis of syphilis. In the present study, we demonstrated that 
      stimulation of HDVSMCs with T. pallidum resulted in the upregulated gene 
      transcription and protein expression of interleukin (IL)-6, monocyte 
      chemoattractant protein-1 (MCP-1), and intercellular adhesion molecule-1 (ICAM-1) 
      in a dose- and time-dependent manner. Moreover, the migration and adhesion of 
      THP-1 cells to HDVSMCs were significantly suppressed by anti-MCP-1 and 
      anti-ICAM-1 neutralizing antibodies, respectively. Further studies revealed that 
      T. pallidum activated the NF-kappaB signaling pathway in HDVSMCs. Inhibition of NF-kappaB 
      suppressed T. pallidum-induced IL-6, MCP-1, and ICAM-1 expression. In addition, 
      the migration and adhesion of THP-1 cells to T. pallidum-treated HDVSMCs were 
      significantly decreased by pretreatment with an NF-kappaB inhibitor. These findings 
      demonstrate that T. pallidum induces the production of IL-6, MCP-1, and ICAM-1 in 
      HDVSMCs and promotes the adherence and migration of THP-1 cells to HDVSMCs 
      through the NF-kappaB signaling pathway, which may provide new insight into the 
      pathogenesis of T. pallidum infection.
FAU - Gao, Zheng-Xiang
AU  - Gao ZX
AD  - Center of Clinical Laboratory, Zhongshan Hospital, School of Medicine, Xiamen 
      University, Xiamen, China.
FAU - Liu, Li-Li
AU  - Liu LL
AD  - Center of Clinical Laboratory, Zhongshan Hospital, School of Medicine, Xiamen 
      University, Xiamen, China.
AD  - Institute of Infectious Disease, School of Medicine, Xiamen University, Xiamen, 
      China.
FAU - Lin, Li-Rong
AU  - Lin LR
AD  - Center of Clinical Laboratory, Zhongshan Hospital, School of Medicine, Xiamen 
      University, Xiamen, China.
AD  - Institute of Infectious Disease, School of Medicine, Xiamen University, Xiamen, 
      China.
FAU - Tong, Man-Li
AU  - Tong ML
AD  - Center of Clinical Laboratory, Zhongshan Hospital, School of Medicine, Xiamen 
      University, Xiamen, China.
AD  - Institute of Infectious Disease, School of Medicine, Xiamen University, Xiamen, 
      China.
FAU - Liu, Fan
AU  - Liu F
AD  - Center of Clinical Laboratory, Zhongshan Hospital, School of Medicine, Xiamen 
      University, Xiamen, China.
AD  - Institute of Infectious Disease, School of Medicine, Xiamen University, Xiamen, 
      China.
FAU - Yang, Tian-Ci
AU  - Yang TC
AD  - Center of Clinical Laboratory, Zhongshan Hospital, School of Medicine, Xiamen 
      University, Xiamen, China.
AD  - Institute of Infectious Disease, School of Medicine, Xiamen University, Xiamen, 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190621
PL  - Switzerland
TA  - Front Cell Infect Microbiol
JT  - Frontiers in cellular and infection microbiology
JID - 101585359
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 0 (ICAM1 protein, human)
RN  - 0 (Interleukin-6)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Messenger)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Antibodies, Neutralizing
MH  - *Bodily Secretions
MH  - *Cell Adhesion
MH  - *Cell Movement
MH  - Chemokine CCL2/metabolism
MH  - Cytokines/*metabolism
MH  - Humans
MH  - Intercellular Adhesion Molecule-1
MH  - Interleukin-6/metabolism
MH  - Myocytes, Smooth Muscle
MH  - NF-kappa B/metabolism
MH  - RNA, Messenger/metabolism
MH  - Signal Transduction
MH  - Syphilis/immunology
MH  - *THP-1 Cells
MH  - Treponema pallidum/*metabolism/pathogenicity
PMC - PMC6598120
OTO - NOTNLM
OT  - Treponema pallidum
OT  - adherence
OT  - cytokine
OT  - human dermal vascular smooth muscle cells
OT  - migration
EDAT- 2019/07/12 06:00
MHDA- 2020/02/19 06:00
PMCR- 2019/01/01
CRDT- 2019/07/12 06:00
PHST- 2019/03/12 00:00 [received]
PHST- 2019/06/07 00:00 [accepted]
PHST- 2019/07/12 06:00 [entrez]
PHST- 2019/07/12 06:00 [pubmed]
PHST- 2020/02/19 06:00 [medline]
PHST- 2019/01/01 00:00 [pmc-release]
AID - 10.3389/fcimb.2019.00220 [doi]
PST - epublish
SO  - Front Cell Infect Microbiol. 2019 Jun 21;9:220. doi: 10.3389/fcimb.2019.00220. 
      eCollection 2019.