PMID- 31294067
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 2352-3409 (Electronic)
IS  - 2352-3409 (Linking)
VI  - 25
DP  - 2019 Aug
TI  - Transcriptome profiling data reveals ubiquitin-specific peptidase 9 knockdown 
      effects.
PG  - 104130
LID - 10.1016/j.dib.2019.104130 [doi]
LID - 104130
AB  - Ubiquitin specific peptidase 9 (USP9) is a deubiquitinase encoded by a sex-linked 
      gene with a Y-chromosomal form (USP9Y) and an X-chromosomal form (USP9X) that 
      escapes X-inactivation. Since USP9 is a key regulatory gene with sex-linked 
      expression in the human brain, the gene may be of interest for researchers 
      studying molecular gender differences and ubiquitin signaling in the brain. To 
      assess the downstream effects of knocking down USP9X and USP9Y on a 
      transcriptome-wide scale, we have conducted microarray profiling experiments 
      using the human DU145 prostate cancer cell culture model, after confirming the 
      robust expression of both USP9X and USP9Y in this model. By designing shRNA 
      constructs for the specific knockdown of USP9X and the joint knockdown of USP9X 
      and USP9Y, we have compared gene expression changes in both knockdowns to control 
      conditions to infer potential shared and X- or Y-form specific alterations. Here, 
      we provide details of the corresponding microarray profiling data, which has been 
      deposited in the Gene Expression Omnibus database (GEO series accession number 
      GSE79376). A biological interpretation of the data in the context of a potential 
      involvement of USP9 in Alzheimer's disease has previously been presented in 
      Koglsberger et al. (2016). To facilitate the re-use and re-analysis of the data 
      for other applications, e.g. the study of ubiquitin signaling and protein 
      turnover control, and the regulation of molecular gender differences in the human 
      brain and brain-related disorders, we provide a more in-depth discussion of the 
      data properties, specifications and possible use cases.
FAU - Glaab, Enrico
AU  - Glaab E
AD  - Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, 7 
      avenue des Hauts Fourneaux, L-4362 Esch-sur-Alzette, Luxembourg.
FAU - Antony, Paul
AU  - Antony P
AD  - Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, 7 
      avenue des Hauts Fourneaux, L-4362 Esch-sur-Alzette, Luxembourg.
FAU - Koglsberger, Sandra
AU  - Koglsberger S
AD  - Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, 7 
      avenue des Hauts Fourneaux, L-4362 Esch-sur-Alzette, Luxembourg.
FAU - Forster, Julia Ilona
AU  - Forster JI
AD  - Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, 7 
      avenue des Hauts Fourneaux, L-4362 Esch-sur-Alzette, Luxembourg.
FAU - Cordero-Maldonado, Maria Lorena
AU  - Cordero-Maldonado ML
AD  - Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, 7 
      avenue des Hauts Fourneaux, L-4362 Esch-sur-Alzette, Luxembourg.
FAU - Crawford, Alexander
AU  - Crawford A
AD  - Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Oslo, 
      Norway.
FAU - Garcia, Pierre
AU  - Garcia P
AD  - Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, 7 
      avenue des Hauts Fourneaux, L-4362 Esch-sur-Alzette, Luxembourg.
FAU - Buttini, Manuel
AU  - Buttini M
AD  - Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, 7 
      avenue des Hauts Fourneaux, L-4362 Esch-sur-Alzette, Luxembourg.
LA  - eng
PT  - Journal Article
DEP - 20190611
PL  - Netherlands
TA  - Data Brief
JT  - Data in brief
JID - 101654995
PMC - PMC6595415
OTO - NOTNLM
OT  - Cell culture
OT  - Knockdown
OT  - Microarray
OT  - Transcriptomics
OT  - Ubiquitin signaling
EDAT- 2019/07/12 06:00
MHDA- 2019/07/12 06:01
PMCR- 2019/06/11
CRDT- 2019/07/12 06:00
PHST- 2018/12/13 00:00 [received]
PHST- 2019/04/26 00:00 [revised]
PHST- 2019/06/03 00:00 [accepted]
PHST- 2019/07/12 06:00 [entrez]
PHST- 2019/07/12 06:00 [pubmed]
PHST- 2019/07/12 06:01 [medline]
PHST- 2019/06/11 00:00 [pmc-release]
AID - S2352-3409(19)30484-6 [pii]
AID - 104130 [pii]
AID - 10.1016/j.dib.2019.104130 [doi]
PST - epublish
SO  - Data Brief. 2019 Jun 11;25:104130. doi: 10.1016/j.dib.2019.104130. eCollection 
      2019 Aug.