PMID- 31294637
OWN - NLM
STAT- MEDLINE
DCOM- 20210930
LR  - 20210930
IS  - 1607-8438 (Electronic)
IS  - 0300-8207 (Linking)
VI  - 61
IP  - 6
DP  - 2020 Nov
TI  - Naringin alleviates H(2)O(2)-induced apoptosis via the PI3K/Akt pathway in rat 
      nucleus pulposus-derived mesenchymal stem cells.
PG  - 554-567
LID - 10.1080/03008207.2019.1631299 [doi]
AB  - Purpose: To investigate the protective effect of naringin (Nar) on 
      H(2)O(2)-induced apoptosis of nucleus pulposus-derived mesenchymal stem cells 
      (NPMSC) and the potential mechanism in this process. Methods: Rat NPMSC were 
      cultured in MSC culture medium or culture medium with different concentrations of 
      H(2)O(2). Nar or the combination of Nar and LY294002 was added into the culture 
      medium to investigate the effects of Nar. Cell viability was evaluated by cell 
      counting kit-8 (CCK-8) assay. The apoptosis rate was determined using Annexin 
      V/PI dual staining and terminal deoxynucleotide transferase-mediated dUTP nick 
      end labeling (TUNEL) assays. Additionally, the levels of reactive oxygen species 
      (ROS) and mitochondrial membrane potential (MMP) were analyzed by flow cytometry. 
      ATP level in NPMSC was analyzed via ATP detection kit. Mitochondrial 
      ultrastructure change was observed through transmission electron microscope 
      (TEM). Levels of apoptosis-associated molecules (cleaved caspase-3, Bax and 
      Bcl-2) were evaluated via RT-PCR and western blot, respectively. Results: The 
      cells isolated from NP met the criteria for MSC. H(2)O(2) significantly promoted 
      NPMSC apoptosis in a dose and time-dependent manner. Nar showed no cytotoxicity 
      effect on NPMSC up to a concentration of 100 muM for 24 h. Nar exhibited 
      protective effects against H(2)O(2)-induced NPMSC apoptosis including apoptosis 
      rate, expressions of proapoptosis and antiapoptosis related genes and protein. 
      Nar could also alleviate H(2)O(2)-induced mitochondrial dysfunction of increased 
      mitochondrial ROS production, reduced MMP, decreased intracellular ATP and 
      mitochondrial ultrastructure change. However, these protected effects were 
      inhibited after LY294002 treatment. Conclusions: Our results demonstrated that 
      Nar efficiently attenuated H(2)O(2)-induced NPMSC apoptosis and mitochondrial 
      dysfunction. The activation of ROS-mediated PI3K/Akt pathway may be the potential 
      mechanism in this process.
FAU - Nan, Li-Ping
AU  - Nan LP
AD  - Department of Orthopedics, Dalian Medical University , Dalian, Liaoning, China.
AD  - Department of Orthopedics, Clinical Medical College of Yangzhou University , 
      Yangzhou, Jiangsu, China.
FAU - Wang, Feng
AU  - Wang F
AD  - Department of Orthopedics, Dalian Medical University , Dalian, Liaoning, China.
AD  - Department of Orthopedics, Clinical Medical College of Yangzhou University , 
      Yangzhou, Jiangsu, China.
FAU - Ran, Di
AU  - Ran D
AD  - College of Veterinary Medicine, Yangzhou University , Yangzhou, China.
FAU - Zhou, Shi-Feng
AU  - Zhou SF
AD  - Department of Orthopedics, Clinical Medical College of Yangzhou University , 
      Yangzhou, Jiangsu, China.
FAU - Liu, Yang
AU  - Liu Y
AD  - Department of Orthopedics, Dalian Medical University , Dalian, Liaoning, China.
AD  - Department of Orthopedics, Clinical Medical College of Yangzhou University , 
      Yangzhou, Jiangsu, China.
FAU - Zhang, Zhen
AU  - Zhang Z
AD  - Department of Orthopedics, Dalian Medical University , Dalian, Liaoning, China.
AD  - Department of Orthopedics, Clinical Medical College of Yangzhou University , 
      Yangzhou, Jiangsu, China.
FAU - Huang, Ze-Nan
AU  - Huang ZN
AD  - Department of Orthopedics, Clinical Medical College of Yangzhou University , 
      Yangzhou, Jiangsu, China.
FAU - Wang, Ze-Yu
AU  - Wang ZY
AD  - Department of Orthopedics, Clinical Medical College of Yangzhou University , 
      Yangzhou, Jiangsu, China.
FAU - Wang, Jing-Cheng
AU  - Wang JC
AD  - Department of Orthopedics, Clinical Medical College of Yangzhou University , 
      Yangzhou, Jiangsu, China.
FAU - Feng, Xin-Min
AU  - Feng XM
AD  - Department of Orthopedics, Clinical Medical College of Yangzhou University , 
      Yangzhou, Jiangsu, China.
FAU - Zhang, Liang
AU  - Zhang L
AD  - Department of Orthopedics, Clinical Medical College of Yangzhou University , 
      Yangzhou, Jiangsu, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190711
PL  - England
TA  - Connect Tissue Res
JT  - Connective tissue research
JID - 0365263
RN  - 0 (Flavanones)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - N7TD9J649B (naringin)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Animals
MH  - *Apoptosis/drug effects
MH  - Cell Survival/drug effects
MH  - Flavanones/chemistry/*pharmacology
MH  - Hydrogen Peroxide/*toxicity
MH  - Mesenchymal Stem Cells/drug effects/*pathology/ultrastructure
MH  - Mitochondria/drug effects/pathology/ultrastructure
MH  - Models, Biological
MH  - Nucleus Pulposus/*pathology
MH  - Phosphatidylinositol 3-Kinases/*metabolism
MH  - Proto-Oncogene Proteins c-akt/*metabolism
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction/drug effects
OTO - NOTNLM
OT  - Naringin
OT  - PI3K/Akt pathway
OT  - intervertebral disc degeneration
OT  - nucleus pulposus-derived mesenchymal stem cells
OT  - oxidative stress
EDAT- 2019/07/12 06:00
MHDA- 2021/10/01 06:00
CRDT- 2019/07/12 06:00
PHST- 2019/07/12 06:00 [pubmed]
PHST- 2021/10/01 06:00 [medline]
PHST- 2019/07/12 06:00 [entrez]
AID - 10.1080/03008207.2019.1631299 [doi]
PST - ppublish
SO  - Connect Tissue Res. 2020 Nov;61(6):554-567. doi: 10.1080/03008207.2019.1631299. 
      Epub 2019 Jul 11.