PMID- 31295089
OWN - NLM
STAT- MEDLINE
DCOM- 20191206
LR  - 20191217
IS  - 1938-5404 (Electronic)
IS  - 0033-7587 (Linking)
VI  - 192
IP  - 3
DP  - 2019 Sep
TI  - RITCARD: Radiation-Induced Tracks, Chromosome Aberrations, Repair and Damage.
PG  - 282-298
LID - 10.1667/RR15250.1 [doi]
AB  - Chromosome aberrations (CAs) are one of the effects of radiation exposure and can 
      have implications for human health in the space environment, since they are 
      related to cancer risk. In radiation research, chromosome aberrations are a 
      convenient biomarker for carcinogenesis. To shed light on the formation and 
      quality of chromosome aberrations in the space environment, many experiments and 
      simulations have been performed using chromosome aberrations in human cells, 
      induced by heavy ions, which are present in galactic cosmic rays (GCRs). In this 
      work, the new simulation program, radiation-induced tracks, chromosome 
      aberrations, repair and damage (RITCARD), is presented. This software program is 
      based on the algorithm used in the NASA Radiation Track Image (NASARTI) model 
      with some improvements. NASARTI and RITCARD are both comprised of four parts: a 
      random walk (RW) algorithm for simulating chromosomes in a nucleus; a 
      deoxyribonucleic acid (DNA) damage algorithm; a break repair process; and a 
      function to assess and count chromosome aberrations. Prior to running RITCARD, 
      the code, relativistic ion tracks (RITRACKS), is used to simulate detailed 
      radiation track structure and calculate time-dependent differential voxel dose 
      maps in a parallelepiped centered on a cell nucleus. The RITCARD program reads 
      the pre-calculated voxel dose and locates the intersections between the voxels 
      and the chromosomes that were simulated by random walk. Radiation-induced breaks 
      occur strictly at these intersections with a probability that is a function of 
      the voxel dose. When a break occurs in the random walk, the corresponding 
      chromosome piece is cut into two fragments where each has a free end at the 
      position of the break. RITCARD generates a collection of all fragments, free 
      ends, and enlists free end pairs. In the next step, the algorithm simulates the 
      time-dependent rejoining of free end pairs, using different probabilities for 
      pairs originating from a given break (proper) or from different breaks 
      (improper), which results in the formation of fragment sequences. By grouping 
      these sequences, the program determines the number and types of aberrations, 
      based on the same criteria used in our experiment. The new program is used to 
      assess the yields of various types of chromosome aberrations in human fibroblast 
      cells for several ions ((1)H(+), (4)He(2+), (12)C(6+), (16)O(8+), (20)Ne(10+), 
      (28)Si(14+), (48)Ti(22+) and (56)Fe(26+)) with energies varying from 10 to 1,000 
      MeV/n. The results show linear and linear-quadratic dose dependence for most 
      chromosome aberrations types. The calculation results were compared with those 
      obtained by fluorescence in situ hybridization (FISH) experiments that were 
      performed by our group. The simulations and experiments are in better agreement 
      at lower LET. Regarding the simulation results, the coefficient of the linear 
      part of the dose-dependence curve also peaks at an LET value of approximately 100 
      keV/lm, which evokes a relative biological effectiveness (RBE) peak found by 
      other researchers.
FAU - Plante, Ianik
AU  - Plante I
AD  - KBRWyle, Houston, Texas 77058.
FAU - Ponomarev, Artem
AU  - Ponomarev A
AD  - KBRWyle, Houston, Texas 77058.
FAU - Patel, Zarana
AU  - Patel Z
AD  - KBRWyle, Houston, Texas 77058.
FAU - Slaba, Tony
AU  - Slaba T
AD  - NASA Langley Research Center, Hampton, Virginia 23681.
FAU - Hada, Megumi
AU  - Hada M
AD  - Prairie View A&M University, Prairie View, Texas 77446.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20190711
PL  - United States
TA  - Radiat Res
JT  - Radiation research
JID - 0401245
SB  - IM
MH  - Cell Line
MH  - Cell Nucleus/genetics/radiation effects
MH  - Chromosome Aberrations/*radiation effects
MH  - *DNA Damage
MH  - DNA Repair/*radiation effects
MH  - Extraterrestrial Environment
MH  - Humans
MH  - Kinetics
MH  - *Models, Genetic
MH  - Programming Languages
EDAT- 2019/07/12 06:00
MHDA- 2019/12/18 06:00
CRDT- 2019/07/12 06:00
PHST- 2019/07/12 06:00 [pubmed]
PHST- 2019/12/18 06:00 [medline]
PHST- 2019/07/12 06:00 [entrez]
AID - 10.1667/RR15250.1 [pii]
AID - 10.1667/RR15250.1 [doi]
PST - ppublish
SO  - Radiat Res. 2019 Sep;192(3):282-298. doi: 10.1667/RR15250.1. Epub 2019 Jul 11.