PMID- 31295581
OWN - NLM
STAT- MEDLINE
DCOM- 20210210
LR  - 20210210
IS  - 2213-7173 (Electronic)
IS  - 2213-7165 (Linking)
VI  - 20
DP  - 2020 Mar
TI  - Faecal carriage of high-level aminoglycoside-resistant and ampicillin-resistant 
      Enterococcus species in healthy Iranian children.
PG  - 135-144
LID - S2213-7165(19)30166-3 [pii]
LID - 10.1016/j.jgar.2019.06.022 [doi]
AB  - OBJECTIVES: High-level aminoglycoside, ampicillin and vancomycin resistance and 
      virulence genes among enterococcal isolates collected from healthy middle-school 
      children in Ardabil, Iran, during 2016 were investigated. METHODS: Totally, 305 
      faecal specimens were collected. Isolates underwent antimicrobial susceptibility 
      testing, virulence gene detection and molecular typing. RESULTS: Totally, 409 
      enterococcal isolates were collected, comprising Enterococcus faecium (235; 
      57.5%), Enterococcus faecalis (56; 13.7%) and other Enterococcus spp. (118; 
      28.9%). Overall, 71 (17.4%), 11 (2.7%) and 10 (2.4%) isolates were identified as 
      high-level streptomycin-resistant (HLSR), high-level gentamicin-resistant (HLGR) 
      and ampicillin-resistant (AR), respectively. Among HLSR isolates, 40 (56.3%), 5 
      (7.0%) and 26 (36.6%) were E. faecium, E. faecalis and other Enterococcus spp., 
      respectively. Among HLGR isolates 4 (36.4%) and 7 (63.6%) and among AR isolates 7 
      (70.0%) and 3 (30.0%) were E. faecium and other Enterococcus spp., respectively. 
      Accordingly, 21.6%, 3.6% and 3.3% of subjects were colonised with HLSR, HLGR and 
      AR Enterococcus spp. Carriage of HLGR, HLSR and AR isolates was associated with 
      prior antibiotic consumption (P</=0.05). Additionally, male sex and antacid 
      consumption were associated with AR enterococcal carriage. Moreover, 69 (97.2%), 
      10 (90.9%) and 9 (90.0%) of HLSR, HLGR and AR isolates were multidrug-resistant, 
      respectively. No vancomycin-resistant enterococci were detected. ERIC-PCR 
      revealed high genetic diversity among isolates. gelE and asa1 were major 
      virulence genes both in E. faecalis and E. faecium. Presence of gelE was 
      associated with HLSR and HLGR phenotypes (P</=0.05). CONCLUSION: Community 
      intestinal carriage of HLSR enterococci was high; however, carriage of HLGR and 
      AR enterococci was low.
CI  - Copyright (c) 2019 International Society for Antimicrobial Chemotherapy. Published 
      by Elsevier Ltd. All rights reserved.
FAU - Jannati, Elham
AU  - Jannati E
AD  - Department of Biology, Science and Research Branch, Islamic Azad University, 
      Tehran, Iran.
FAU - Amirmozaffari, Nour
AU  - Amirmozaffari N
AD  - Department of Microbiology, School of Medicine, Iran University of Medical 
      Sciences, Tehran, Iran.
FAU - Saadatmand, Sara
AU  - Saadatmand S
AD  - Department of Biology, Science and Research Branch, Islamic Azad University, 
      Tehran, Iran.
FAU - Arzanlou, Mohsen
AU  - Arzanlou M
AD  - Department of Microbiology, School of Medicine, Ardabil University of Medical 
      Sciences, Ardabil, Iran. Electronic address: m.arzanlou@arums.ac.ir.
LA  - eng
PT  - Journal Article
DEP - 20190708
PL  - Netherlands
TA  - J Glob Antimicrob Resist
JT  - Journal of global antimicrobial resistance
JID - 101622459
RN  - 0 (Aminoglycosides)
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Virulence Factors)
RN  - 7C782967RD (Ampicillin)
SB  - IM
MH  - Adolescent
MH  - Aminoglycosides/pharmacology
MH  - Ampicillin/pharmacology
MH  - Anti-Bacterial Agents/*pharmacology
MH  - Carrier State/*epidemiology/microbiology
MH  - *Drug Resistance, Bacterial
MH  - Enterococcus/classification/*drug effects/genetics/isolation & purification
MH  - Enterococcus faecalis/drug effects
MH  - Enterococcus faecium/drug effects
MH  - Feces/*microbiology
MH  - Female
MH  - Healthy Volunteers
MH  - Humans
MH  - Iran/epidemiology
MH  - Male
MH  - Microbial Sensitivity Tests
MH  - Sex Characteristics
MH  - Virulence Factors/genetics
OTO - NOTNLM
OT  - Ampicillin resistance
OT  - Enterococci
OT  - Faecal carriage
OT  - HLAR
OT  - Healthy children
OT  - Virulence genes
EDAT- 2019/07/12 06:00
MHDA- 2021/02/11 06:00
CRDT- 2019/07/12 06:00
PHST- 2019/01/18 00:00 [received]
PHST- 2019/06/27 00:00 [revised]
PHST- 2019/06/29 00:00 [accepted]
PHST- 2019/07/12 06:00 [pubmed]
PHST- 2021/02/11 06:00 [medline]
PHST- 2019/07/12 06:00 [entrez]
AID - S2213-7165(19)30166-3 [pii]
AID - 10.1016/j.jgar.2019.06.022 [doi]
PST - ppublish
SO  - J Glob Antimicrob Resist. 2020 Mar;20:135-144. doi: 10.1016/j.jgar.2019.06.022. 
      Epub 2019 Jul 8.