PMID- 31298404
OWN - NLM
STAT- MEDLINE
DCOM- 20201001
LR  - 20211204
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 23
IP  - 12
DP  - 2019 Jun
TI  - Nicotinamide protects chronic hypoxic myocardial cells through regulating mTOR 
      pathway and inducing autophagy.
PG  - 5503-5511
LID - 18220 [pii]
LID - 10.26355/eurrev_201906_18220 [doi]
AB  - OBJECTIVE: To determine the protective effect of nicotinamide on chronic hypoxic 
      myocardial cells and its underlying mechanism. MATERIALS AND METHODS: The H9C2 
      cell lines were taken as objects of study, and were divided into blank group, 
      hypoxia group and nicotinamide treatment group. The cell viability, apoptosis 
      level, autophagy level and mammalian target of rapamycin (mTOR) pathway activity 
      in each group were detected via Cell Counting Kit-8 (CCK8) assay, Hoechst 
      staining, immunofluorescence staining, Polymerase Chain Reaction (PCR) and 
      Western blotting, respectively. RESULTS: Nicotinamide could protect the viability 
      of normoxic and chronic hypoxic myocardial cells. Besides, it could also inhibit 
      the expression of caspase3 messenger ribonucleic acid (mRNA) in chronic hypoxic 
      myocardial cells, and reduce the expression of apoptosis-related proteins. 
      Furthermore, it could induce the mRNA expression of autophagy-associated gene 5 
      (ATG5) and increase the expression of autophagy-related proteins. Further study 
      on the mechanism of nicotinamide showed that nicotinamide could inhibit the 
      activity of the mTOR pathway, thus regulating the autophagy. CONCLUSIONS: 
      Nicotinamide induces the autophagy of chronic hypoxic myocardial cells by 
      regulating the mTOR pathway, thereby protecting cells from apoptosis.
FAU - Li, W
AU  - Li W
AD  - Department of Cardiology, Taizhou People's Hospital, Taizhou, Jiangsu, China. 
      userliwei@126.com.
FAU - Zhu, L
AU  - Zhu L
FAU - Ruan, Z-B
AU  - Ruan ZB
FAU - Wang, M-X
AU  - Wang MX
FAU - Ren, Y
AU  - Ren Y
FAU - Lu, W
AU  - Lu W
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (Atg5 protein, rat)
RN  - 0 (Autophagy-Related Protein 5)
RN  - 0 (Cardiotonic Agents)
RN  - 25X51I8RD4 (Niacinamide)
RN  - EC 2.7.1.1 (mTOR protein, rat)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 3.4.22.- (Casp3 protein, rat)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Autophagy/*drug effects
MH  - Autophagy-Related Protein 5/metabolism
MH  - Cardiotonic Agents/*pharmacology
MH  - Caspase 3/metabolism
MH  - Cell Hypoxia/physiology
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Humans
MH  - Myocardial Ischemia/pathology/prevention & control
MH  - Myocytes, Cardiac/*drug effects/pathology
MH  - Niacinamide/*pharmacology/therapeutic use
MH  - Rats
MH  - TOR Serine-Threonine Kinases/*metabolism
EDAT- 2019/07/13 06:00
MHDA- 2020/10/02 06:00
CRDT- 2019/07/13 06:00
PHST- 2019/07/13 06:00 [entrez]
PHST- 2019/07/13 06:00 [pubmed]
PHST- 2020/10/02 06:00 [medline]
AID - 18220 [pii]
AID - 10.26355/eurrev_201906_18220 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2019 Jun;23(12):5503-5511. doi: 
      10.26355/eurrev_201906_18220.