PMID- 31298480 OWN - NLM STAT- MEDLINE DCOM- 20200602 LR - 20200602 IS - 1521-6551 (Electronic) IS - 1521-6543 (Linking) VI - 71 IP - 11 DP - 2019 Nov TI - Mass spectrometry-based proteomic analysis of FSCN1-interacting proteins in laryngeal squamous cell carcinoma cells. PG - 1771-1784 LID - 10.1002/iub.2121 [doi] AB - Fascin actin-bundling protein 1 (FSCN1) is an evolutionarily conserved actin-bundling protein that plays a critical role in cell migration, motility, adhesion, and cellular interactions. Although multiple clinical studies have implicated the expression of FSCN1 in laryngeal squamous cell carcinoma (LSCC) progression, the precise mechanism of FSCN1 in the process has not been clearly elucidated. To define FSCN1 function, we characterized FSCN1-interacting proteins in two cell lines by immunoprecipitation followed by mass spectrometry (MS). After data filtering, 119 proteins with expression in both the Hep-2 and TU-177 cell samples were identified as FSCN1-interacting partners. With in-depth bioinformatics analysis, we linked FSCN1 to critical cellular processes including cell adhesion, glycolysis/gluconeogenesis, regulation of protein ubiquitination, ribosomal RNA processing, and small molecule metabolism. We discuss the interactions between FSCN1 and some of the newly validated partners. The identification of these potential partners of FSCN1 expands our knowledge of the FSCN1 interactome and provides a valuable resource for understanding the functions of this protein in LSCC progression. CI - (c) 2019 International Union of Biochemistry and Molecular Biology. FAU - Liu, Hongliang AU - Liu H AUID- ORCID: 0000-0003-0123-870X AD - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical University, Taiyuan, China. AD - Otolaryngology Head & Neck Surgery Research Institute, Shanxi Medical University, Taiyuan, China. AD - The Key Scientific and Technological Innovation Platform for Precision Diagnosis and Treatment of Head and Neck Cancer, Shanxi Province, Shanxi Medical University, Taiyuan, China. FAU - Cui, Jiajia AU - Cui J AD - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical University, Taiyuan, China. AD - Otolaryngology Head & Neck Surgery Research Institute, Shanxi Medical University, Taiyuan, China. AD - The Key Scientific and Technological Innovation Platform for Precision Diagnosis and Treatment of Head and Neck Cancer, Shanxi Province, Shanxi Medical University, Taiyuan, China. FAU - Zhang, Yuliang AU - Zhang Y AD - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical University, Taiyuan, China. AD - Otolaryngology Head & Neck Surgery Research Institute, Shanxi Medical University, Taiyuan, China. AD - The Key Scientific and Technological Innovation Platform for Precision Diagnosis and Treatment of Head and Neck Cancer, Shanxi Province, Shanxi Medical University, Taiyuan, China. FAU - Niu, Min AU - Niu M AD - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical University, Taiyuan, China. AD - Otolaryngology Head & Neck Surgery Research Institute, Shanxi Medical University, Taiyuan, China. AD - The Key Scientific and Technological Innovation Platform for Precision Diagnosis and Treatment of Head and Neck Cancer, Shanxi Province, Shanxi Medical University, Taiyuan, China. FAU - Xue, Xuting AU - Xue X AD - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical University, Taiyuan, China. AD - Otolaryngology Head & Neck Surgery Research Institute, Shanxi Medical University, Taiyuan, China. AD - The Key Scientific and Technological Innovation Platform for Precision Diagnosis and Treatment of Head and Neck Cancer, Shanxi Province, Shanxi Medical University, Taiyuan, China. FAU - Yin, Hongyu AU - Yin H AD - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical University, Taiyuan, China. AD - Otolaryngology Head & Neck Surgery Research Institute, Shanxi Medical University, Taiyuan, China. AD - The Key Scientific and Technological Innovation Platform for Precision Diagnosis and Treatment of Head and Neck Cancer, Shanxi Province, Shanxi Medical University, Taiyuan, China. FAU - Tang, Yemei AU - Tang Y AD - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical University, Taiyuan, China. AD - Otolaryngology Head & Neck Surgery Research Institute, Shanxi Medical University, Taiyuan, China. AD - The Key Scientific and Technological Innovation Platform for Precision Diagnosis and Treatment of Head and Neck Cancer, Shanxi Province, Shanxi Medical University, Taiyuan, China. FAU - Dai, Li AU - Dai L AD - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical University, Taiyuan, China. AD - Otolaryngology Head & Neck Surgery Research Institute, Shanxi Medical University, Taiyuan, China. AD - The Key Scientific and Technological Innovation Platform for Precision Diagnosis and Treatment of Head and Neck Cancer, Shanxi Province, Shanxi Medical University, Taiyuan, China. FAU - Dai, Fengsheng AU - Dai F AD - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical University, Taiyuan, China. AD - Otolaryngology Head & Neck Surgery Research Institute, Shanxi Medical University, Taiyuan, China. AD - The Key Scientific and Technological Innovation Platform for Precision Diagnosis and Treatment of Head and Neck Cancer, Shanxi Province, Shanxi Medical University, Taiyuan, China. FAU - Guo, Yujia AU - Guo Y AD - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical University, Taiyuan, China. AD - Otolaryngology Head & Neck Surgery Research Institute, Shanxi Medical University, Taiyuan, China. AD - The Key Scientific and Technological Innovation Platform for Precision Diagnosis and Treatment of Head and Neck Cancer, Shanxi Province, Shanxi Medical University, Taiyuan, China. FAU - Wu, Yongyan AU - Wu Y AUID- ORCID: 0000-0003-1669-3860 AD - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical University, Taiyuan, China. AD - Otolaryngology Head & Neck Surgery Research Institute, Shanxi Medical University, Taiyuan, China. AD - The Key Scientific and Technological Innovation Platform for Precision Diagnosis and Treatment of Head and Neck Cancer, Shanxi Province, Shanxi Medical University, Taiyuan, China. FAU - Gao, Wei AU - Gao W AUID- ORCID: 0000-0001-7836-2851 AD - Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Shanxi Medical University, Taiyuan, China. AD - Otolaryngology Head & Neck Surgery Research Institute, Shanxi Medical University, Taiyuan, China. AD - The Key Scientific and Technological Innovation Platform for Precision Diagnosis and Treatment of Head and Neck Cancer, Shanxi Province, Shanxi Medical University, Taiyuan, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20190712 PL - England TA - IUBMB Life JT - IUBMB life JID - 100888706 RN - 0 (Carrier Proteins) RN - 0 (FSCN1 protein, human) RN - 0 (Microfilament Proteins) RN - 0 (Proteome) SB - IM EIN - IUBMB Life. 2020 Feb;72(2):305. PMID: 31943741 MH - Carcinoma, Squamous Cell/*metabolism/pathology MH - Carrier Proteins/*metabolism MH - *Gene Expression Regulation, Neoplastic MH - Humans MH - Laryngeal Neoplasms/*metabolism/pathology MH - Mass Spectrometry/*methods MH - Microfilament Proteins/*metabolism MH - Protein Interaction Maps MH - Proteome/*analysis MH - Tumor Cells, Cultured OTO - NOTNLM OT - FSCN1 OT - coimmunoprecipitation OT - interacting partners OT - laryngeal squamous cell carcinoma OT - mass spectrometry OT - proteomics EDAT- 2019/07/13 06:00 MHDA- 2020/06/03 06:00 CRDT- 2019/07/13 06:00 PHST- 2019/05/06 00:00 [received] PHST- 2019/06/02 00:00 [accepted] PHST- 2019/07/13 06:00 [pubmed] PHST- 2020/06/03 06:00 [medline] PHST- 2019/07/13 06:00 [entrez] AID - 10.1002/iub.2121 [doi] PST - ppublish SO - IUBMB Life. 2019 Nov;71(11):1771-1784. doi: 10.1002/iub.2121. Epub 2019 Jul 12.