PMID- 31299475 OWN - NLM STAT- MEDLINE DCOM- 20191017 LR - 20191017 IS - 1090-2414 (Electronic) IS - 0147-6513 (Linking) VI - 182 DP - 2019 Oct 30 TI - Transcriptional effects in the estuarine guppy Poecilia vivipara exposed to sanitary sewage in laboratory and in situ. PG - 109411 LID - S0147-6513(19)30741-9 [pii] LID - 10.1016/j.ecoenv.2019.109411 [doi] AB - The urban growth has increased sanitary sewage discharges in coastal ecosystems, negatively affecting the aquatic biota. Mangroves, one of the most human-affected coastal biomes, are areas for reproduction and nursing of several species. In order to evaluate the effects of sanitary sewage effluents in mangrove species, this study assessed the hepatic transcriptional responses of guppy fish Poecilia vivipara exposed to sanitary sewage 33% (v:v), using suppressive subtraction hybridization (SSH), high throughput sequencing of RNA (Ion-proton) and quantification of transcript levels by qPCR of some identified genes in fish kept in a sewage-contaminated environment. Genes identified are related predominantly to xenobiotic biotransformation, immune system and sexual differentiation. The qPCR results confirmed the induction of cytochrome P450 1A (CYP1A), glutathione S transferase A-like (GST A-like) methyltransferase (MET) and UDP glycosyltransferase 1A (UDPGT1A), and repression of complement component C3 (C3), doublesex and mab-3 related transcription factor 1 (DMRT1), and transferrin (TF) in the laboratory experiment. In the field exposure, the transcript levels of CYP1A, DMRT1, MET, GST A-like and UDPGT1A were higher in fishes exposed at the contaminated sites compared to the reference site. Chemical analysis in fish from the laboratory and in situ experiments, and surface sediment from the sewage-contaminated sites revealed relevant levels of polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyl (PCBs) and linear alkylbenzenes (LABs). These data reinforce the use of P. vivipara as a sentinel for monitoring environmental contamination in coastal regions. CI - Copyright (c) 2019 Elsevier Inc. All rights reserved. FAU - Piazza, Clei Endrigo AU - Piazza CE AD - Laboratory of Biomarkers of Aquatic Contamination and Immunochemistry, Department of Biochemistry, Federal University of Santa Catarina, UFSC, Florianopolis, SC, Brazil. FAU - Mattos, Jaco Joaquim AU - Mattos JJ AD - Aquaculture Pathology Research, NEPAQ, Federal University of Santa Catarina, Florianopolis, SC, Brazil. FAU - de Toledo-Silva, Guilherme AU - de Toledo-Silva G AD - Bioinformatics Laboratory, Cell Biology, Embriology and Genetics Department, Federal University of Santa Catarina, Florianopolis, SC, Brazil. FAU - Flores-Nunes, Fabricio AU - Flores-Nunes F AD - Laboratory of Biomarkers of Aquatic Contamination and Immunochemistry, Department of Biochemistry, Federal University of Santa Catarina, UFSC, Florianopolis, SC, Brazil. FAU - Tadra-Sfeir, Michelle Zibetti AU - Tadra-Sfeir MZ AD - Department of Biochemistry, Federal University of Parana, Curitiba, Parana, Brazil. FAU - Trevisan, Rafael AU - Trevisan R AD - Nicholas School of the Environment, Duke University, Durham, NC, 27708, USA. FAU - Bittencourt, Arnaldo Cechinel AU - Bittencourt AC AD - Laboratory of Biomarkers of Aquatic Contamination and Immunochemistry, Department of Biochemistry, Federal University of Santa Catarina, UFSC, Florianopolis, SC, Brazil. FAU - Bicego, Marcia Caruso AU - Bicego MC AD - Laboratory of Marine Organic Chemistry, Oceanographic Institute, University of Sao Paulo, Sao Paulo, Brazil. FAU - Taniguchi, Satie AU - Taniguchi S AD - Laboratory of Marine Organic Chemistry, Oceanographic Institute, University of Sao Paulo, Sao Paulo, Brazil. FAU - Marques, Maria Risoleta Freire AU - Marques MRF AD - Laboratory of Biomarkers of Aquatic Contamination and Immunochemistry, Department of Biochemistry, Federal University of Santa Catarina, UFSC, Florianopolis, SC, Brazil. FAU - Dafre, Alcir Luiz AU - Dafre AL AD - Laboratory of Cellular Defenses, Department of Biochemistry, Federal University of Santa Catarina, Florianopolis, Brazil. FAU - Bianchini, Adalto AU - Bianchini A AD - Department of Physiological Sciences, Federal University of Rio Grande Foundation, Rio Grande, Brazil. FAU - Souza, Emanuel Maltempi de AU - Souza EM AD - Department of Biochemistry, Federal University of Parana, Curitiba, Parana, Brazil. FAU - Bainy, Afonso Celso Dias AU - Bainy ACD AD - Laboratory of Biomarkers of Aquatic Contamination and Immunochemistry, Department of Biochemistry, Federal University of Santa Catarina, UFSC, Florianopolis, SC, Brazil. Electronic address: afonso.bainy@ufsc.br. LA - eng PT - Journal Article DEP - 20190709 PL - Netherlands TA - Ecotoxicol Environ Saf JT - Ecotoxicology and environmental safety JID - 7805381 RN - 0 (Sewage) RN - 0 (Water Pollutants, Chemical) RN - 0 (Xenobiotics) SB - IM MH - Animals MH - Biotransformation MH - Environmental Monitoring/*methods MH - Estuaries MH - Liver/*drug effects/metabolism MH - Models, Theoretical MH - Poecilia/*genetics/metabolism MH - Sewage/*chemistry MH - Transcription, Genetic/*drug effects MH - Water Pollutants, Chemical/metabolism/*toxicity MH - Xenobiotics/metabolism OTO - NOTNLM OT - Biomarkers OT - Biotransformation OT - Endocrine disruptors OT - Ion proton OT - Mangrove OT - Suppressive subtraction hybridization EDAT- 2019/07/13 06:00 MHDA- 2019/10/18 06:00 CRDT- 2019/07/13 06:00 PHST- 2019/03/18 00:00 [received] PHST- 2019/06/28 00:00 [revised] PHST- 2019/07/02 00:00 [accepted] PHST- 2019/07/13 06:00 [pubmed] PHST- 2019/10/18 06:00 [medline] PHST- 2019/07/13 06:00 [entrez] AID - S0147-6513(19)30741-9 [pii] AID - 10.1016/j.ecoenv.2019.109411 [doi] PST - ppublish SO - Ecotoxicol Environ Saf. 2019 Oct 30;182:109411. doi: 10.1016/j.ecoenv.2019.109411. Epub 2019 Jul 9.