PMID- 31300043
OWN - NLM
STAT- MEDLINE
DCOM- 20191209
LR  - 20221207
IS  - 1297-9716 (Electronic)
IS  - 0928-4249 (Print)
IS  - 0928-4249 (Linking)
VI  - 50
IP  - 1
DP  - 2019 Jul 12
TI  - Matrine inhibits IL-1beta secretion in primary porcine alveolar macrophages through 
      the MyD88/NF-kappaB pathway and NLRP3 inflammasome.
PG  - 53
LID - 10.1186/s13567-019-0671-x [doi]
LID - 53
AB  - Our previous studies demonstrated that matrine directly acts on the replication 
      process of porcine reproductive and respiratory syndrome virus (PRRSV). Matrine 
      inhibits viral replication and is also associated with the NF-kappaB signalling 
      pathway. These results suggest that matrine has antiviral and anti-inflammatory 
      effects. However, the specific anti-inflammatory mechanism of matrine is still 
      unclear. In this study, we investigated the anti-IL-1beta mechanism of matrine, as 
      IL-1beta is a major inflammatory cytokine, in porcine alveolar macrophages (PAMs) 
      stimulated with 4 mug PRRSV 5'-untranslated region (UTR) RNA and 1 mug/mL LPS. 
      After 5'UTR RNA and LPS co-stimulation of PAMs for 12 h, the expression of IL-1beta, 
      IL-6, IL-8 and TNF-alpha was significantly increased. The results also showed that 
      co-stimulation induced the expression of MyD88, and activated the NF-kappaB 
      signalling pathway and NLRP3 inflammasome. Furthermore, matrine treatment 
      downregulated MyD88, NLRP3 and caspase-1 expression, inhibited ASC speck 
      formation, suppressed IkappaBalpha phosphorylation, and interfered with the translocation 
      of NF-kappaB from the cytoplasm to the nucleus. These results suggest that matrine 
      plays an important role in PAMs co-stimulated with PRRSV 5'UTR RNA and LPS via 
      its effect on NF-kappaB and the NLRP3 inflammasome. These findings lay the foundation 
      for the exploration of the clinical application of matrine in PRRSV disease.
FAU - Sun, Panpan
AU  - Sun P
AD  - College of Animal Science and Veterinary Medicine, Shanxi Agricultural 
      University, Taigu, 030801, Shanxi, People's Republic of China.
AD  - School of Life Science and Engineering, Foshan University, Foshan, 528000, 
      Guangdong, People's Republic of China.
FAU - Sun, Na
AU  - Sun N
AD  - College of Animal Science and Veterinary Medicine, Shanxi Agricultural 
      University, Taigu, 030801, Shanxi, People's Republic of China.
FAU - Yin, Wei
AU  - Yin W
AD  - College of Animal Science and Veterinary Medicine, Shanxi Agricultural 
      University, Taigu, 030801, Shanxi, People's Republic of China.
FAU - Sun, Yaogui
AU  - Sun Y
AD  - College of Animal Science and Veterinary Medicine, Shanxi Agricultural 
      University, Taigu, 030801, Shanxi, People's Republic of China.
FAU - Fan, Kuohai
AU  - Fan K
AD  - Laboratory Animal Center, Shanxi Agricultural University, Taigu, 030801, Shanxi, 
      People's Republic of China.
FAU - Guo, Jianhua
AU  - Guo J
AD  - Department of Veterinary Pathobiology, Schubot Exotic Bird Health Center, Texas 
      A&M University, College Station, TX, 77843, USA.
FAU - Khan, Ajab
AU  - Khan A
AD  - College of Animal Science and Veterinary Medicine, Shanxi Agricultural 
      University, Taigu, 030801, Shanxi, People's Republic of China.
FAU - He, Yongming
AU  - He Y
AD  - School of Life Science and Engineering, Foshan University, Foshan, 528000, 
      Guangdong, People's Republic of China. ymhe@fosu.edu.cn.
FAU - Li, Hongquan
AU  - Li H
AUID- ORCID: 0000-0002-2030-5587
AD  - College of Animal Science and Veterinary Medicine, Shanxi Agricultural 
      University, Taigu, 030801, Shanxi, People's Republic of China. livets@163.com.
LA  - eng
GR  - 31702285/National Natural Science Foundation of China/
GR  - 2017YFD0501500/National Key R&D Program of China/
GR  - 2017001/the Scientific Innovation Project of Shanxi Agricultural University/
PT  - Journal Article
DEP - 20190712
PL  - England
TA  - Vet Res
JT  - Veterinary research
JID - 9309551
RN  - 0 (Alkaloids)
RN  - 0 (Inflammasomes)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (NF-kappa B)
RN  - 0 (NLR Family, Pyrin Domain-Containing 3 Protein)
RN  - 0 (Quinolizines)
RN  - 0 (RNA, Viral)
RN  - 0 (Matrines)
SB  - IM
MH  - Alkaloids/*pharmacology
MH  - Animals
MH  - Inflammasomes/*immunology
MH  - Interleukin-1beta/*metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - Macrophages/*drug effects/immunology
MH  - Myeloid Differentiation Factor 88/immunology
MH  - NF-kappa B/*immunology
MH  - NLR Family, Pyrin Domain-Containing 3 Protein/immunology
MH  - Porcine respiratory and reproductive syndrome virus/physiology
MH  - Quinolizines/*pharmacology
MH  - RNA, Viral/genetics
MH  - Signal Transduction/immunology
MH  - Sus scrofa
MH  - Transfection/veterinary
MH  - Matrines
PMC - PMC6626430
COIS- The authors declare that they have no competing interests.
EDAT- 2019/07/14 06:00
MHDA- 2019/12/18 06:00
PMCR- 2019/07/12
CRDT- 2019/07/14 06:00
PHST- 2019/03/12 00:00 [received]
PHST- 2019/06/22 00:00 [accepted]
PHST- 2019/07/14 06:00 [entrez]
PHST- 2019/07/14 06:00 [pubmed]
PHST- 2019/12/18 06:00 [medline]
PHST- 2019/07/12 00:00 [pmc-release]
AID - 10.1186/s13567-019-0671-x [pii]
AID - 671 [pii]
AID - 10.1186/s13567-019-0671-x [doi]
PST - epublish
SO  - Vet Res. 2019 Jul 12;50(1):53. doi: 10.1186/s13567-019-0671-x.