PMID- 31301204
OWN - NLM
STAT- MEDLINE
DCOM- 20191216
LR  - 20200108
IS  - 1365-2672 (Electronic)
IS  - 1364-5072 (Linking)
VI  - 127
IP  - 5
DP  - 2019 Nov
TI  - Facultative and anaerobic consortia of haloalkaliphilic ureolytic micro-organisms 
      capable of precipitating calcium carbonate.
PG  - 1479-1489
LID - 10.1111/jam.14384 [doi]
AB  - AIMS: Development of biomineralization technologies has largely focused on 
      microbially induced carbonate precipitation (MICP) via Sporosarcina pasteurii 
      ureolysis; however, as an obligate aerobe, the general utility of this organism 
      is limited. Here, facultative and anaerobic haloalkaliphiles capable of ureolysis 
      were enriched, identified and then compared to S. pasteurii regarding 
      biomineralization activities. METHODS AND RESULTS: Anaerobic and facultative 
      enrichments for haloalkaliphilic and ureolytic micro-organisms were established 
      from sediment slurries collected at Soap Lake (WA). Optimal pH, temperature and 
      salinity were determined for highly ureolytic enrichments, with dominant 
      populations identified via a combination of high-throughput SSU rRNA gene 
      sequencing, clone libraries and Sanger sequencing of isolates. The enrichment 
      cultures consisted primarily of Sporosarcina- and Clostridium-like organisms. 
      Ureolysis rates and direct cell counts in the enrichment cultures were comparable 
      to the S. pasteurii (strain ATCC 11859) type strain. CONCLUSIONS: Ureolysis rates 
      from both facultatively and anaerobically enriched haloalkaliphiles were either 
      not statistically significantly different to, or statistically significantly 
      higher than, the S. pasteurii (strain ATCC 11859) rates. Work here concludes that 
      extreme environments can harbour highly ureolytic active bacteria with potential 
      advantages for large scale applications, such as environments devoid of oxygen. 
      SIGNIFICANCE AND IMPACT OF THE STUDY: The bacterial consortia and isolates 
      obtained add to the possible suite of organisms available for MICP 
      implementation, therefore potentially improving the economics and efficiency of 
      commercial biomineralization.
CI  - (c) 2019 The Society for Applied Microbiology.
FAU - Skorupa, D J
AU  - Skorupa DJ
AUID- ORCID: 0000-0001-9007-5919
AD  - Department of Chemical and Biological Engineering, Montana State University, 
      Bozeman, MT, USA.
AD  - Center for Biofilm Engineering, Montana State University, Bozeman, MT, USA.
FAU - Akyel, A
AU  - Akyel A
AUID- ORCID: 0000-0003-2474-2335
AD  - Department of Chemical and Biological Engineering, Montana State University, 
      Bozeman, MT, USA.
AD  - Center for Biofilm Engineering, Montana State University, Bozeman, MT, USA.
FAU - Fields, M W
AU  - Fields MW
AD  - Center for Biofilm Engineering, Montana State University, Bozeman, MT, USA.
AD  - Department of Microbiology and Immunology, Montana State University, Bozeman, MT, 
      USA.
FAU - Gerlach, R
AU  - Gerlach R
AD  - Department of Chemical and Biological Engineering, Montana State University, 
      Bozeman, MT, USA.
AD  - Center for Biofilm Engineering, Montana State University, Bozeman, MT, USA.
LA  - eng
SI  - GENBANK/SRP127176
SI  - GENBANK/MG682464
SI  - GENBANK/MG382488
SI  - GENBANK/MG674285
SI  - GENBANK/MG674286
GR  - DE-FG02-13ER86571/U.S. Department of Energy/
PT  - Journal Article
DEP - 20190819
PL  - England
TA  - J Appl Microbiol
JT  - Journal of applied microbiology
JID - 9706280
RN  - 0 (Alkalies)
RN  - 8W8T17847W (Urea)
RN  - H0G9379FGK (Calcium Carbonate)
SB  - IM
MH  - Alkalies/*metabolism
MH  - Anaerobiosis
MH  - Bacteria/metabolism
MH  - Calcium Carbonate/chemistry/*metabolism
MH  - Geologic Sediments/microbiology
MH  - Microbial Consortia
MH  - Sporosarcina/genetics/isolation & purification/*metabolism
MH  - Urea/*metabolism
OTO - NOTNLM
OT  - bio-inventory
OT  - biomineralization
OT  - calcium carbonate
OT  - haloalkaliphile
OT  - ureolysis activity
EDAT- 2019/07/14 06:00
MHDA- 2019/12/18 06:00
CRDT- 2019/07/14 06:00
PHST- 2018/12/12 00:00 [received]
PHST- 2019/06/12 00:00 [revised]
PHST- 2019/07/10 00:00 [accepted]
PHST- 2019/07/14 06:00 [pubmed]
PHST- 2019/12/18 06:00 [medline]
PHST- 2019/07/14 06:00 [entrez]
AID - 10.1111/jam.14384 [doi]
PST - ppublish
SO  - J Appl Microbiol. 2019 Nov;127(5):1479-1489. doi: 10.1111/jam.14384. Epub 2019 
      Aug 19.