PMID- 31301234 OWN - NLM STAT- MEDLINE DCOM- 20200427 LR - 20200427 IS - 1365-3040 (Electronic) IS - 0140-7791 (Linking) VI - 42 IP - 11 DP - 2019 Nov TI - Natural variation in a calreticulin gene causes reduced resistance to Ca(2+) deficiency-induced tipburn in Chinese cabbage (Brassica rapa ssp. pekinensis). PG - 3044-3060 LID - 10.1111/pce.13612 [doi] AB - Tipburn is an irreversible physiological disorder of Chinese cabbage that decreases crop value. Because of a strong environmental component, tipburn-resistant cultivars are the only solution, although tipburn resistance genes are unknown in Chinese cabbage. We studied three populations of Chinese cabbage over four growing seasons under field conditions: (a) 194 diverse inbred lines, (b) a doubled haploid (DH100) population, and (c) an F(2) population. The 194 lines were genotyped using single nucleotide polymorphism markers, and genome-wide-association mapping showed that 24 gQTLs were significantly associated with tipburn disease index. Analysis of the DH100 and F(2) populations identified a shared tipburn-associated locus, gqbTRA06, that was found to cover the region defined by one of the 24 gQTLs. Of 35 genes predicted in the 0.14-Mb quantitative trait locus region, Bra018575 (calreticulin family protein, BrCRT2) showed higher expression levels during disease development. We cloned the two BrCRT2 alleles from tipburn-resistant (BrCRT2(R) ) and tipburn-susceptible (BrCRT2(S) ) lines and identified a 51-bp deletion in BrCRT2(S) . Overexpression of BrCRT2(R) increased Ca(2+) storage in the Arabidopsis crt2 mutant and also reduced cell death in leaf tips and margins under Ca(2+) -depleted conditions. Our results suggest that BrCRT2 is a possible candidate gene for controlling tipburn in Chinese cabbage. CI - (c) 2019 John Wiley & Sons Ltd. FAU - Su, Tongbing AU - Su T AUID- ORCID: 0000-0002-3411-7341 AD - Beijing Vegetable Research Center (BVRC), Beijing Academy of Agriculture and Forestry Science (BAAFS), Beijing, 100097, China. AD - National Engineering Research Center for Vegetables, Beijing, 100097, China. AD - Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Beijing, 100097, China. AD - Beijing Key Laboratory of Vegetable Germplasm Improvement, Beijing, 100097, China. FAU - Li, Peirong AU - Li P AD - Beijing Vegetable Research Center (BVRC), Beijing Academy of Agriculture and Forestry Science (BAAFS), Beijing, 100097, China. AD - National Engineering Research Center for Vegetables, Beijing, 100097, China. AD - Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Beijing, 100097, China. AD - Beijing Key Laboratory of Vegetable Germplasm Improvement, Beijing, 100097, China. FAU - Wang, Huiping AU - Wang H AD - National Engineering Research Center for Vegetables, Beijing, 100097, China. FAU - Wang, Weihong AU - Wang W AD - Beijing Vegetable Research Center (BVRC), Beijing Academy of Agriculture and Forestry Science (BAAFS), Beijing, 100097, China. AD - National Engineering Research Center for Vegetables, Beijing, 100097, China. AD - Beijing Key Laboratory of Vegetable Germplasm Improvement, Beijing, 100097, China. FAU - Zhao, Xiuyun AU - Zhao X AD - Beijing Vegetable Research Center (BVRC), Beijing Academy of Agriculture and Forestry Science (BAAFS), Beijing, 100097, China. AD - National Engineering Research Center for Vegetables, Beijing, 100097, China. AD - Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Beijing, 100097, China. FAU - Yu, Yangjun AU - Yu Y AD - Beijing Vegetable Research Center (BVRC), Beijing Academy of Agriculture and Forestry Science (BAAFS), Beijing, 100097, China. AD - National Engineering Research Center for Vegetables, Beijing, 100097, China. AD - Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Beijing, 100097, China. AD - Beijing Key Laboratory of Vegetable Germplasm Improvement, Beijing, 100097, China. FAU - Zhang, Deshuang AU - Zhang D AD - Beijing Vegetable Research Center (BVRC), Beijing Academy of Agriculture and Forestry Science (BAAFS), Beijing, 100097, China. AD - National Engineering Research Center for Vegetables, Beijing, 100097, China. AD - Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Beijing, 100097, China. AD - Beijing Key Laboratory of Vegetable Germplasm Improvement, Beijing, 100097, China. FAU - Yu, Shuancang AU - Yu S AD - Beijing Vegetable Research Center (BVRC), Beijing Academy of Agriculture and Forestry Science (BAAFS), Beijing, 100097, China. AD - National Engineering Research Center for Vegetables, Beijing, 100097, China. AD - Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Beijing, 100097, China. AD - Beijing Key Laboratory of Vegetable Germplasm Improvement, Beijing, 100097, China. FAU - Zhang, Fenglan AU - Zhang F AD - Beijing Vegetable Research Center (BVRC), Beijing Academy of Agriculture and Forestry Science (BAAFS), Beijing, 100097, China. AD - National Engineering Research Center for Vegetables, Beijing, 100097, China. AD - Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Beijing, 100097, China. AD - Beijing Key Laboratory of Vegetable Germplasm Improvement, Beijing, 100097, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20190801 PL - United States TA - Plant Cell Environ JT - Plant, cell & environment JID - 9309004 RN - 0 (Calreticulin) RN - 0 (Plant Proteins) RN - SY7Q814VUP (Calcium) SB - IM MH - Arabidopsis/genetics MH - Brassica rapa/*genetics MH - Calcium/metabolism MH - Calreticulin/*genetics MH - Cell Death MH - Chromosome Mapping MH - Gene Expression Regulation, Plant MH - Genetic Predisposition to Disease/*genetics MH - *Genetic Variation MH - Genome, Plant MH - Genome-Wide Association Study MH - Genotype MH - Phenotype MH - Plant Diseases/*genetics MH - Plant Leaves MH - Plant Proteins/genetics MH - Plants, Genetically Modified MH - Polymorphism, Single Nucleotide MH - Quantitative Trait Loci MH - Sequence Analysis, DNA MH - Sequence Analysis, Protein OTO - NOTNLM OT - calcium OT - calreticulin OT - genome-wide association mapping OT - quantitative trait locus EDAT- 2019/07/14 06:00 MHDA- 2020/04/28 06:00 CRDT- 2019/07/14 06:00 PHST- 2019/01/25 00:00 [received] PHST- 2019/07/08 00:00 [revised] PHST- 2019/07/09 00:00 [accepted] PHST- 2019/07/14 06:00 [pubmed] PHST- 2020/04/28 06:00 [medline] PHST- 2019/07/14 06:00 [entrez] AID - 10.1111/pce.13612 [doi] PST - ppublish SO - Plant Cell Environ. 2019 Nov;42(11):3044-3060. doi: 10.1111/pce.13612. Epub 2019 Aug 1.