PMID- 31304602
OWN - NLM
STAT- MEDLINE
DCOM- 20201016
LR  - 20201016
IS  - 1097-4652 (Electronic)
IS  - 0021-9541 (Linking)
VI  - 235
IP  - 2
DP  - 2020 Feb
TI  - Adhesion G-protein coupled receptor 56 is required for 3T3-L1 adipogenesis.
PG  - 1601-1614
LID - 10.1002/jcp.29079 [doi]
AB  - Obesity-associated conditions represent major global health and financial burdens 
      and understanding processes regulating adipogenesis could lead to novel 
      intervention strategies. This study shows that adhesion G-protein coupled 
      receptor 56 (GPR56) gene transcripts are reduced in abdominal visceral white 
      adipose tissue derived from obese Zucker rats versus lean controls. 
      Immunostaining in 3T3-L1 preadipocytes reveals both mitotic cell restricted 
      surface and low level general expression patterns of Gpr56. Gpr56 transcripts are 
      differentially expressed in 3T3-L1 cells during adipogenesis. Transient knockdown 
      (KD) of Gpr56 in 3T3-L1 cells dramatically inhibits differentiation through 
      reducing the accumulation of both neutral cellular lipids (56%) and production of 
      established adipogenesis Ppargamma (2) (60-80%), C/ebpalpha (40-78%) mediator, and Ap2 
      (56-80%) marker proteins. Furthermore, genome editing of Gpr56 in 3T3-L1 cells 
      created CW2.2.4 and RM4.2.5.5 clones (Gpr56 (-/-) cells) with compound 
      heterozygous deletion frameshift mutations which abolish adipogenesis. Genome 
      edited cells have sustained levels of the adipogenesis inhibitor beta-catenin, 
      reduced proliferation, reduced adhesion, altered profiles, and or abundance of 
      extracellular matrix component gene transcripts for fibronectin, types I, III, 
      and IV collagens and loss of actin stress fibers. beta-catenin KD alone is 
      insufficient to restore adipogenesis in Gpr56 (-/-) cells. Together these data 
      show that Gpr56 is required for adipogenesis in 3T3-L1 cells. This report is the 
      first demonstration that Gpr56 participates in regulation of the adipogenesis 
      developmental program. Modulation of the levels of this protein and/or its 
      biological activity may represent a novel target for development of therapeutic 
      agents for the treatment of obesity.
CI  - (c) 2019 Wiley Periodicals, Inc.
FAU - Al Hasan, Mohammad
AU  - Al Hasan M
AD  - Department of Biological & Biomedical Sciences, School of Health & Life Sciences, 
      Glasgow Caledonian University, Glasgow, Scotland.
FAU - Roy, Poornima
AU  - Roy P
AD  - Department of Biological & Biomedical Sciences, School of Health & Life Sciences, 
      Glasgow Caledonian University, Glasgow, Scotland.
FAU - Dolan, Sharron
AU  - Dolan S
AD  - Department of Biological & Biomedical Sciences, School of Health & Life Sciences, 
      Glasgow Caledonian University, Glasgow, Scotland.
FAU - Martin, Patricia E
AU  - Martin PE
AUID- ORCID: 0000-0003-0890-8059
AD  - Department of Biological & Biomedical Sciences, School of Health & Life Sciences, 
      Glasgow Caledonian University, Glasgow, Scotland.
FAU - Patterson, Steven
AU  - Patterson S
AD  - Department of Biological & Biomedical Sciences, School of Health & Life Sciences, 
      Glasgow Caledonian University, Glasgow, Scotland.
FAU - Bartholomew, Chris
AU  - Bartholomew C
AUID- ORCID: 0000-0002-1153-8389
AD  - Department of Biological & Biomedical Sciences, School of Health & Life Sciences, 
      Glasgow Caledonian University, Glasgow, Scotland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190715
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (Adgrg1 protein, rat)
RN  - 0 (GPR56 protein, mouse)
RN  - 0 (Receptors, G-Protein-Coupled)
SB  - IM
MH  - 3T3-L1 Cells
MH  - Adipocytes/*metabolism
MH  - Adipogenesis/*physiology
MH  - Animals
MH  - Gene Knockdown Techniques
MH  - Male
MH  - Mice
MH  - Obesity/metabolism
MH  - Rats
MH  - Rats, Zucker
MH  - Receptors, G-Protein-Coupled/*metabolism
OTO - NOTNLM
OT  - GPR56
OT  - adipogenesis
OT  - extracellular matrix
OT  - genome editing
OT  - knockdown
OT  - beta-catenin
EDAT- 2019/07/16 06:00
MHDA- 2020/10/21 06:00
CRDT- 2019/07/16 06:00
PHST- 2019/04/09 00:00 [received]
PHST- 2019/06/14 00:00 [accepted]
PHST- 2019/07/16 06:00 [pubmed]
PHST- 2020/10/21 06:00 [medline]
PHST- 2019/07/16 06:00 [entrez]
AID - 10.1002/jcp.29079 [doi]
PST - ppublish
SO  - J Cell Physiol. 2020 Feb;235(2):1601-1614. doi: 10.1002/jcp.29079. Epub 2019 Jul 
      15.