PMID- 31306659
OWN - NLM
STAT- MEDLINE
DCOM- 20191007
LR  - 20191007
IS  - 1879-0631 (Electronic)
IS  - 0024-3205 (Linking)
VI  - 232
DP  - 2019 Sep 1
TI  - MitoQ ameliorates testicular damage induced by gamma irradiation in rats: 
      Modulation of mitochondrial apoptosis and steroidogenesis.
PG  - 116655
LID - S0024-3205(19)30581-8 [pii]
LID - 10.1016/j.lfs.2019.116655 [doi]
AB  - AIMS: The deleterious effect of gamma radiation on testicular tissue is a 
      challenging problem in nuclear medicine. This study investigated the potential 
      radioprotective effect of mitoquinol (MitoQ), a mitochondria-targeted 
      antioxidant, against testicular damage induced by gamma irradiation in rats. MAIN 
      METHODS: Rats were allocated into four groups. The first group served as the 
      control, the second group received MitoQ (2 mg / kg / day; i.p.) for seven days, 
      the third group was exposed to gamma radiation (5 Gy as a single dose) and the 
      last group received MitoQ prior to irradiation. Rats were sacrificed. Then, sperm 
      analyses and the serum testosterone were determined. Moreover, evaluation of 
      mitochondrial oxidative stress parameters (SOD, GSH and GPx) as well as apoptosis 
      indicators (cytochrome-c, Bax, Bcl-2 and caspase-3) was performed. Additionally, 
      analysis of steroidogensis biomarkers (StAR, 3beta-HSD and 17beta-HSD) and 
      histopathological investigations were carried out. KEY FINDINGS: MitoQ 
      replenished mitochondrial SOD, GPx and GSH indicating its strong antioxidant 
      effect in addition to its energy preservation manifested by the elevated ATP. 
      MitoQ inhibited the intrinsic apoptosis via diminution of Bax, cytochrome-c and 
      caspase-3 and alleviation of Bcl-2. This antioxidant conferred protection to 
      steroidogenesis as verified by the increase in testosterone and the up-regulation 
      of StAR, 3beta-HSD and 17beta-HSD expression; these effects might be correlated with 
      its antioxidant/anti-apoptotic potential. Histopathological and sperm analyses 
      corroborated the biochemical findings. SIGNIFICANCE: This study identifies MitoQ 
      as a novel agent for the management of testicular toxicity induced by gamma 
      irradiation.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - Ibrahim, Ayman A
AU  - Ibrahim AA
AD  - Drug Radiation Research Department, National Center for Radiation Research and 
      Technology (NCRRT), Egyptian Atomic Energy Authority, P. O. Box 29, Nasr City, 
      Cairo 11787, Egypt. Electronic address: ayman.ibrahim@eaea.org.eg.
FAU - Karam, Heba M
AU  - Karam HM
AD  - Drug Radiation Research Department, National Center for Radiation Research and 
      Technology (NCRRT), Egyptian Atomic Energy Authority, P. O. Box 29, Nasr City, 
      Cairo 11787, Egypt.
FAU - Shaaban, Esmat A
AU  - Shaaban EA
AD  - Drug Radiation Research Department, National Center for Radiation Research and 
      Technology (NCRRT), Egyptian Atomic Energy Authority, P. O. Box 29, Nasr City, 
      Cairo 11787, Egypt.
FAU - Safar, Marwa M
AU  - Safar MM
AD  - Department of Pharmacology and Toxicology, Faculty of Pharmacy, Cairo University, 
      Kasr El-Aini St., Cairo 11562, Egypt; Department of Pharmacology and 
      Biochemistry, Faculty of Pharmacy, The British University in Egypt, Suez Desert 
      Road, P.O. Box 43, El Sherouk City, Cairo 11837, Egypt.
FAU - El-Yamany, Mohammed F
AU  - El-Yamany MF
AD  - Department of Pharmacology and Toxicology, Faculty of Pharmacy, Cairo University, 
      Kasr El-Aini St., Cairo 11562, Egypt.
LA  - eng
PT  - Journal Article
DEP - 20190713
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Organophosphorus Compounds)
RN  - 0 (Steroids)
RN  - 1339-63-5 (Ubiquinone)
RN  - 65M41NIO61 (mitoquinol)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Body Weight/drug effects
MH  - *Gamma Rays
MH  - Male
MH  - Mitochondria/*drug effects/metabolism
MH  - Organ Size/drug effects
MH  - Organophosphorus Compounds/*pharmacology
MH  - Oxidative Stress/drug effects
MH  - Rats
MH  - Rats, Wistar
MH  - Spermatogenesis/drug effects/radiation effects
MH  - Steroids/*biosynthesis
MH  - Testis/*drug effects/pathology/*radiation effects
MH  - Ubiquinone/*pharmacology
MH  - Whole-Body Irradiation
OTO - NOTNLM
OT  - Gamma radiation
OT  - Mitochondria-targeted antioxidant
OT  - Mitochondrial apoptosis
OT  - Steroidogenesis
OT  - Testicular damage
EDAT- 2019/07/16 06:00
MHDA- 2019/10/08 06:00
CRDT- 2019/07/16 06:00
PHST- 2019/05/17 00:00 [received]
PHST- 2019/07/11 00:00 [revised]
PHST- 2019/07/12 00:00 [accepted]
PHST- 2019/07/16 06:00 [pubmed]
PHST- 2019/10/08 06:00 [medline]
PHST- 2019/07/16 06:00 [entrez]
AID - S0024-3205(19)30581-8 [pii]
AID - 10.1016/j.lfs.2019.116655 [doi]
PST - ppublish
SO  - Life Sci. 2019 Sep 1;232:116655. doi: 10.1016/j.lfs.2019.116655. Epub 2019 Jul 
      13.