PMID- 31308626 OWN - NLM STAT- MEDLINE DCOM- 20200121 LR - 20220409 IS - 1177-8881 (Electronic) IS - 1177-8881 (Linking) VI - 13 DP - 2019 TI - Ginsenoside Rb1 pretreatment reverses hippocampal changes in BDNF/TrkB mRNA and protein in rats subjected to acute immobilization stress. PG - 2127-2134 LID - 10.2147/DDDT.S201135 [doi] AB - PURPOSE: Episodes of acute emotional or physical stress can have significant adverse effects on the hippocampus. Ginsenoside Rb1, the most predominant ginsenoside present in Panax species, has been reported to show a neuroprotective effect. The purpose of this study was to investigate the influence of ginsenoside Rb1 on plasma corticosterone (CORT) and adrenocorticotropic hormone (ACTH) levels and hippocampal brain-derived neurotrophic factor (BDNF) and tyrosine kinase B (TrkB) levels in rats subjected to acute immobilization stress. METHODS: Wistar rats were divided into controls treated with saline only (N), rats exposed to stress only (M), and rats pretreated with Rb1 (40 mg.kg (-1)) thirty minutes prior to stress exposure (R). In the model, animals were restrained in a plastic immobilizer for 2 h of acute immobilization stress at room temperature. ELISA was used to determine plasma levels of CORT and ACTH. The effect of Rb1 pretreatment on the expression of BDNF and TrkB was determined by immunofluorescence, real-time PCR, and Western blotting analysis. RESULTS: The R group showed significantly increased plasma CORT and ACTH levels compared to the N and M groups. Acute stress stimulation suppressed BDNF and TrkB protein and mRNA expression in the hippocampus; otherwise, Rb1 pretreatment reversed the decreases. CONCLUSION: The results from this study demonstrate that Rb1 pretreatment reverses the decreases in hippocampal BDNF/TrkB and increases the plasma levels of CORT and ACTH, indicating a potential neuroprotective effect of Rb1 against acute stress. FAU - Kang, Xianhui AU - Kang X AD - Department of Anesthesiology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, People's Republic of China. AD - Department of Anesthesiology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, People's Republic of China. FAU - Hong, Wandong AU - Hong W AD - Department of Gastroenterology and Hepatology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, People's Republic of China. FAU - Xie, Kangjie AU - Xie K AD - Department of Anesthesiology, Zhejiang Cancer Hospital, Hangzhou, Zhejiang 310022, People's Republic of China. FAU - Tang, Hongli AU - Tang H AD - Department of Anesthesiology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, People's Republic of China. FAU - Tang, Jingjing AU - Tang J AD - Department of Anesthesiology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, People's Republic of China. FAU - Luo, Shan AU - Luo S AD - Department of Anesthesiology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, People's Republic of China. FAU - Geng, Wujun AU - Geng W AD - Department of Anesthesiology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, People's Republic of China. FAU - Jia, Danyun AU - Jia D AD - Department of Anesthesiology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, People's Republic of China. LA - eng PT - Journal Article DEP - 20190701 PL - New Zealand TA - Drug Des Devel Ther JT - Drug design, development and therapy JID - 101475745 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Ginsenosides) RN - 0 (Neuroprotective Agents) RN - 0 (RNA, Messenger) RN - 7413S0WMH6 (ginsenoside Rb1) RN - 9002-60-2 (Adrenocorticotropic Hormone) RN - EC 2.7.10.1 (Ntrk2 protein, rat) RN - EC 2.7.10.1 (Receptor, trkB) RN - W980KJ009P (Corticosterone) SB - IM MH - Adrenocorticotropic Hormone/blood MH - Animals MH - Brain-Derived Neurotrophic Factor/genetics/*metabolism MH - Corticosterone/blood MH - Ginsenosides/administration & dosage/*pharmacology MH - Hippocampus/*drug effects/metabolism MH - *Immobilization MH - Male MH - Neuroprotective Agents/administration & dosage/*pharmacology MH - RNA, Messenger/*genetics MH - Rats MH - Rats, Sprague-Dawley MH - Receptor, trkB/*genetics MH - *Stress, Psychological/genetics/metabolism PMC - PMC6612975 OTO - NOTNLM OT - BDNF OT - CORT; ACTH OT - TrkB OT - acute immobilization stress OT - ginsenoside Rb1 COIS- The authors declare that they have no competing or conflicts of interest in regard to this work. EDAT- 2019/07/17 06:00 MHDA- 2020/01/22 06:00 PMCR- 2019/07/01 CRDT- 2019/07/17 06:00 PHST- 2019/01/12 00:00 [received] PHST- 2019/05/14 00:00 [accepted] PHST- 2019/07/17 06:00 [entrez] PHST- 2019/07/17 06:00 [pubmed] PHST- 2020/01/22 06:00 [medline] PHST- 2019/07/01 00:00 [pmc-release] AID - 201135 [pii] AID - 10.2147/DDDT.S201135 [doi] PST - epublish SO - Drug Des Devel Ther. 2019 Jul 1;13:2127-2134. doi: 10.2147/DDDT.S201135. eCollection 2019.