PMID- 31310956
OWN - NLM
STAT- MEDLINE
DCOM- 20200211
LR  - 20200211
IS  - 1873-3557 (Electronic)
IS  - 1386-1425 (Linking)
VI  - 223
DP  - 2019 Dec 5
TI  - Turn-on detection of MicroRNA155 based on simple UCNPs-DNA-AuNPs luminescence 
      energy transfer probe and duplex-specific nuclease signal amplification.
PG  - 117345
LID - S1386-1425(19)30735-8 [pii]
LID - 10.1016/j.saa.2019.117345 [doi]
AB  - A novel luminescence energy transfer (LET) probe for detection of tumor related 
      microRNAs using NaGdF(4):Yb,Er@NaYF(4) upconversion nanoparticles (UCNPs) as 
      energy donors and gold nanoparticles (AuNPs) as energy acceptors was developed. 
      Using the double modified complementary DNA sequences of microRNA155 (miRNA155) 
      as a bridge, NaGdF(4):Yb,Er@NaYF(4) UCNPs and AuNPs were conjugated to form 
      NaGdF(4):Yb,Er@NaYF(4) UCNPs-DNA-AuNPs nanocomplexes (UCNPs-DNA-AuNPs) probe. The 
      energy transfer would occur when the distance between donor and acceptor gets 
      closer. In the presence of target miRNA155, DNA-RNA heteroduplexes appeared as 
      product, but the luminescence intensity was not changed obviously. In the 
      existence of duplex-specific nuclease (DSN), DSN could hydrolyze the DNA strand 
      of DNA-RNA heteroduplexes, the bridge linked NaGdF(4):Yb,Er@NaYF(4) UCNPs and 
      AuNPs was destroyed, which induced that the quenched luminescence intensity was 
      recovered and RNA was released. The released miRNA155 could react with another 
      UCNPs-DNA-AuNPs probe to form DNA-RNA heteroduplexes again. This cyclic reaction 
      generates an amplification of luminescence signal for quantitative detection of 
      miRNA155. Under the illumination of 980 nm laser, the concentration ranges from 
      0.1 nM to 15 nM and the detection of limits was 0.045 nM for detection of 
      miRNA155. Moreover, the UCNPs-DNA-AuNPs probe was used in quantify miRNA155 in 
      cell lysates with satisfactory results.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Lu, Yunyun
AU  - Lu Y
AD  - Anhui Key Laboratory of Chemo-Biosensing, Key Laboratory of Functional Molecular 
      Solids, Ministry of Education, College of Chemistry and Materials Science, Anhui 
      Normal University, Wuhu 241000, PR China.
FAU - Wang, Lun
AU  - Wang L
AD  - Anhui Key Laboratory of Chemo-Biosensing, Key Laboratory of Functional Molecular 
      Solids, Ministry of Education, College of Chemistry and Materials Science, Anhui 
      Normal University, Wuhu 241000, PR China. Electronic address: 
      wanglun@mail.ahnu.edu.cn.
FAU - Chen, Hongqi
AU  - Chen H
AD  - Anhui Key Laboratory of Chemo-Biosensing, Key Laboratory of Functional Molecular 
      Solids, Ministry of Education, College of Chemistry and Materials Science, Anhui 
      Normal University, Wuhu 241000, PR China. Electronic address: 
      hq80chen@mail.ahnu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20190709
PL  - England
TA  - Spectrochim Acta A Mol Biomol Spectrosc
JT  - Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
JID - 9602533
RN  - 0 (MIRN155 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Nucleic Acid Heteroduplexes)
RN  - 7440-57-5 (Gold)
RN  - EC 3.1.- (Endonucleases)
SB  - IM
MH  - Endonucleases/*metabolism
MH  - *Energy Transfer
MH  - Fluorescence Resonance Energy Transfer
MH  - Gold/*chemistry
MH  - Humans
MH  - K562 Cells
MH  - *Luminescence
MH  - Metal Nanoparticles/*chemistry/ultrastructure
MH  - MicroRNAs/genetics/*metabolism
MH  - Nucleic Acid Heteroduplexes/*metabolism
MH  - Particle Size
OTO - NOTNLM
OT  - Detection
OT  - Duplex-specific nuclease
OT  - Gold nanoparticles
OT  - Luminescence energy transfer
OT  - MicroRNA155
OT  - Upconversion nanoparticles
EDAT- 2019/07/17 06:00
MHDA- 2020/02/12 06:00
CRDT- 2019/07/17 06:00
PHST- 2019/02/01 00:00 [received]
PHST- 2019/06/14 00:00 [revised]
PHST- 2019/07/07 00:00 [accepted]
PHST- 2019/07/17 06:00 [pubmed]
PHST- 2020/02/12 06:00 [medline]
PHST- 2019/07/17 06:00 [entrez]
AID - S1386-1425(19)30735-8 [pii]
AID - 10.1016/j.saa.2019.117345 [doi]
PST - ppublish
SO  - Spectrochim Acta A Mol Biomol Spectrosc. 2019 Dec 5;223:117345. doi: 
      10.1016/j.saa.2019.117345. Epub 2019 Jul 9.