PMID- 31311100
OWN - NLM
STAT- MEDLINE
DCOM- 20191209
LR  - 20200225
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 20
IP  - 14
DP  - 2019 Jul 15
TI  - Interleukin (IL)-22 from IL-20 Subfamily of Cytokines Induces Colonic Epithelial 
      Cell Proliferation Predominantly through ERK1/2 Pathway.
LID - 10.3390/ijms20143468 [doi]
LID - 3468
AB  - The interleukin (IL)-20 subfamily of cytokines consists of IL-19, IL-20, IL-22, 
      IL-24, and IL-26, and the expression of IL-20, IL-22, and IL-24 is reported to be 
      higher in the colon of patients with ulcerative colitis. Although the receptors 
      for these cytokines are highly expressed in the colon epithelium, their effects 
      on epithelial renewal are not clearly understood. This study evaluated the 
      effects of IL-20, IL-22, and IL-24 in epithelial renewal using the LS174T human 
      colon cancer epithelial cell line. LS174T cells were treated with IL-20, IL-22, 
      and IL-24 (25, 50, and 100 ng/mL) and a live-cell imaging system was used to 
      evaluate the effects on cell proliferation. Following treatment, the signaling 
      pathways contributing to cell proliferation were investigated through Western 
      blotting in LS174T cells and downstream transcriptional changes through qRT-PCR 
      in LS174T cells, and RNA-Seq in primary murine intestinal epithelial cells. Our 
      results demonstrated that only IL-22 promoted LS174T cell proliferation, mediated 
      via extracellular-signal-regulated kinase (ERK)1/2-mediated downstream regulation 
      of p90RSK, c-Jun, and transcriptional changes of TRIM15 and STOM. IL-22 also 
      promoted expression of ERK1/2-independent genes such as DDR2, LCN2, and LRG1, 
      which are known to be involved in cell proliferation and migration. This study 
      suggests that IL-22 induces cell proliferation in highly proliferative cells such 
      as intestinal epithelial cells.
FAU - Moniruzzaman, Md
AU  - Moniruzzaman M
AD  - Faculty of Medicine, The University of Queensland, Brisbane, QLD 4067, Australia.
AD  - Immunopathology Group, Mater Research Institute-The University of Queensland, 
      Translational Research Institute, Brisbane, QLD 4102, Australia.
FAU - Wang, Ran
AU  - Wang R
AD  - Immunopathology Group, Mater Research Institute-The University of Queensland, 
      Translational Research Institute, Brisbane, QLD 4102, Australia.
FAU - Jeet, Varinder
AU  - Jeet V
AD  - Australian Prostate Cancer Research Centre-Queensland, Institute of Health 
      Biomedical Innovation (IHBI), Queensland University of Technology, Brisbane, QLD 
      4102, Australia.
AD  - Macquarie University Centre for the Health Economy, Macquarie University, NSW 
      2109, Australia.
FAU - McGuckin, Michael A
AU  - McGuckin MA
AD  - Faculty of Medicine, Dentistry and Health Sciences, the University of Melbourne, 
      VIC 3052, Australia.
FAU - Hasnain, Sumaira Z
AU  - Hasnain SZ
AD  - Faculty of Medicine, The University of Queensland, Brisbane, QLD 4067, Australia. 
      sumaira.hasnain@mater.uq.edu.au.
AD  - Immunopathology Group, Mater Research Institute-The University of Queensland, 
      Translational Research Institute, Brisbane, QLD 4102, Australia. 
      sumaira.hasnain@mater.uq.edu.au.
AD  - Australian Infectious Disease Research Centre, University of Queensland, 
      Brisbane, QLD 4067, Australia. sumaira.hasnain@mater.uq.edu.au.
LA  - eng
GR  - Career Development Fellowship (S.Z.H)/National Health and Medical Research 
      Council/
GR  - S.Z.H and R.W/Mater Foundation/
GR  - Centennial Scholarship (M. M)/University of Queensland/
GR  - Research Training Program Scholarship/Australian Government Department of 
      Education and Training/
GR  - Frank Clair Scholarship (M. M)/Mater Research/
GR  - Bushell Post‑doctoral Research Fellowship (R.W)/Gastroenterological Society of 
      Australia/
PT  - Journal Article
DEP - 20190715
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Interleukins)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
SB  - IM
MH  - Animals
MH  - Cell Line, Tumor
MH  - *Cell Proliferation
MH  - Cells, Cultured
MH  - Colonic Neoplasms/*metabolism/pathology
MH  - Enterocytes/*metabolism/physiology
MH  - Humans
MH  - Interleukins/genetics/*metabolism
MH  - *MAP Kinase Signaling System
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mitogen-Activated Protein Kinase 1/metabolism
MH  - Mitogen-Activated Protein Kinase 3/metabolism
PMC - PMC6678670
OTO - NOTNLM
OT  - ERK1/2
OT  - IL-20
OT  - IL-22
OT  - IL-24
OT  - cell proliferation
OT  - wound healing
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses, or interpretation of data; in 
      the writing of the manuscript; or in the decision to publish the results.
EDAT- 2019/07/18 06:00
MHDA- 2019/12/18 06:00
PMCR- 2019/07/01
CRDT- 2019/07/18 06:00
PHST- 2019/06/10 00:00 [received]
PHST- 2019/07/12 00:00 [revised]
PHST- 2019/07/12 00:00 [accepted]
PHST- 2019/07/18 06:00 [entrez]
PHST- 2019/07/18 06:00 [pubmed]
PHST- 2019/12/18 06:00 [medline]
PHST- 2019/07/01 00:00 [pmc-release]
AID - ijms20143468 [pii]
AID - ijms-20-03468 [pii]
AID - 10.3390/ijms20143468 [doi]
PST - epublish
SO  - Int J Mol Sci. 2019 Jul 15;20(14):3468. doi: 10.3390/ijms20143468.