PMID- 31311802
OWN - NLM
STAT- MEDLINE
DCOM- 20200302
LR  - 20200309
IS  - 2373-2822 (Electronic)
IS  - 2373-2822 (Linking)
VI  - 6
IP  - 4
DP  - 2019 Jul/Aug
TI  - Calcium Imaging of Parvalbumin Neurons in the Dorsal Root Ganglia.
LID - ENEURO.0349-18.2019 [pii]
LID - 10.1523/ENEURO.0349-18.2019 [doi]
AB  - We investigated the calcium dynamics of dorsal root ganglion (DRG) neurons using 
      transgenic mice to target expression of the genetically encoded calcium indicator 
      (GECI), GCaMP6s, to a subset of neurons containing parvalbumin (PV), a 
      calcium-binding protein present in proprioceptors and low-threshold 
      mechanoreceptors. This study provides the first analysis of GECI calcium 
      transient parameters from large-diameter DRG neurons. Our approach generated 
      calcium transients of consistent shape and time-course, with quantifiable 
      characteristics. Four parameters of calcium transients were determined to vary 
      independently from each other and thus are likely influenced by different 
      calcium-regulating mechanisms: peak amplitude, rise time (RT), decay time, and 
      recovery time. Pooled analysis of 188 neurons demonstrated unimodal 
      distributions, providing evidence that PV+ DRG neurons regulate calcium similarly 
      as a population despite their differences in size, electrical properties, and 
      functional sensitivities. Calcium transients increased in size with elevated 
      extracellular calcium, longer trains of action potentials, and higher stimulation 
      frequencies. RT and decay time increased with the addition of the selective 
      sarco/endoplasmic reticulum calcium ATPases (SERCA) blocker, thapsigargin (TG), 
      while peak amplitude and recovery time remained the same. When elevating bath pH 
      to 8.8 to block plasma-membrane calcium ATPases (PMCA), all measured parameters 
      significantly increased. These results illustrate that GECI calcium transients 
      provide sufficient resolution to detect changes in electrical activity and 
      intracellular calcium concentration, as well as discern information about the 
      activity of specific subclasses of calcium regulatory mechanisms.
CI  - Copyright (c) 2019 Walters et al.
FAU - Walters, Marie C
AU  - Walters MC
AD  - Department of Neuroscience, Cell Biology, and Physiology, Boonshoft School of 
      Medicine, Wright State University, Dayton, OH 45435.
FAU - Sonner, Martha J
AU  - Sonner MJ
AD  - Department of Neuroscience, Cell Biology, and Physiology, Boonshoft School of 
      Medicine, Wright State University, Dayton, OH 45435.
FAU - Myers, Jessica H
AU  - Myers JH
AD  - Department of Neuroscience, Cell Biology, and Physiology, Boonshoft School of 
      Medicine, Wright State University, Dayton, OH 45435.
FAU - Ladle, David R
AU  - Ladle DR
AD  - Department of Neuroscience, Cell Biology, and Physiology, Boonshoft School of 
      Medicine, Wright State University, Dayton, OH 45435 david.ladle@wright.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20190801
PL  - United States
TA  - eNeuro
JT  - eNeuro
JID - 101647362
RN  - 0 (Parvalbumins)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/analysis
MH  - Calcium Signaling/*physiology
MH  - Female
MH  - Ganglia, Spinal/*physiology
MH  - Male
MH  - Mice, Transgenic
MH  - Neurons/*physiology
MH  - Optical Imaging/methods
MH  - Parvalbumins/*physiology
PMC - PMC6709205
OTO - NOTNLM
OT  - DRG
OT  - calcium imaging
OT  - parvalbumin
OT  - sensory
OT  - transgenic
EDAT- 2019/07/18 06:00
MHDA- 2020/03/03 06:00
PMCR- 2019/08/01
CRDT- 2019/07/18 06:00
PHST- 2018/09/05 00:00 [received]
PHST- 2019/06/12 00:00 [revised]
PHST- 2019/06/22 00:00 [accepted]
PHST- 2019/07/18 06:00 [pubmed]
PHST- 2020/03/03 06:00 [medline]
PHST- 2019/07/18 06:00 [entrez]
PHST- 2019/08/01 00:00 [pmc-release]
AID - ENEURO.0349-18.2019 [pii]
AID - eN-NWR-0349-18 [pii]
AID - 10.1523/ENEURO.0349-18.2019 [doi]
PST - epublish
SO  - eNeuro. 2019 Aug 1;6(4):ENEURO.0349-18.2019. doi: 10.1523/ENEURO.0349-18.2019. 
      Print 2019 Jul/Aug.