PMID- 31312919
OWN - NLM
STAT- MEDLINE
DCOM- 20200210
LR  - 20220214
IS  - 1435-1803 (Electronic)
IS  - 0300-8428 (Print)
IS  - 0300-8428 (Linking)
VI  - 114
IP  - 5
DP  - 2019 Jul 16
TI  - Neutrophil extracellular traps and fibrocytes in ST-segment elevation myocardial 
      infarction.
PG  - 33
LID - 10.1007/s00395-019-0740-3 [doi]
LID - 33
AB  - Leukocyte-mediated inflammation is central in atherothrombosis and ST-segment 
      elevation myocardial infarction (STEMI). Neutrophil extracellular traps (NETs) 
      have been shown to enhance atherothrombosis and stimulate fibroblast function. We 
      analyzed the effects of NETs on cardiac remodeling after STEMI. We measured 
      double-stranded (ds)DNA and citrullinated histone H3 (citH3) as NET surrogate 
      markers in human culprit site and femoral blood collected during primary 
      percutaneous coronary intervention (n = 50). Fibrocytes were characterized in 
      whole blood by flow cytometry, and in culprit site thrombi and myocardium by 
      immunofluorescence. To investigate mechanisms of fibrocyte activation, isolated 
      NETs were used to induce fibrocyte responses in vitro. Enzymatic infarct size was 
      assessed using creatine-phosphokinase isoform MB area under the curve. Left 
      ventricular function was measured by transthoracic echocardiography. NET 
      surrogate markers were increased at the culprit site compared to the femoral site 
      and were positively correlated with infarct size and left ventricular dysfunction 
      at follow-up. In vitro, NETs promoted fibrocyte differentiation from monocytes 
      and induced fibrocyte activation. Highly activated fibrocytes accumulated at the 
      culprit site and in the infarct transition zone. Our data suggest that NETs might 
      be important mediators of fibrotic remodeling after STEMI, possibly by 
      stimulating fibrocytes.
FAU - Hofbauer, Thomas M
AU  - Hofbauer TM
AUID- ORCID: 0000-0001-5324-6336
AD  - Division of Cardiology, Department of Internal Medicine II, Medical University of 
      Vienna, Waehringer Guertel 18-20, 1090, Vienna, Austria.
FAU - Mangold, Andreas
AU  - Mangold A
AD  - Division of Cardiology, Department of Internal Medicine II, Medical University of 
      Vienna, Waehringer Guertel 18-20, 1090, Vienna, Austria.
FAU - Scherz, Thomas
AU  - Scherz T
AD  - Division of Cardiology, Department of Internal Medicine II, Medical University of 
      Vienna, Waehringer Guertel 18-20, 1090, Vienna, Austria.
FAU - Seidl, Veronika
AU  - Seidl V
AD  - Division of Cardiology, Department of Internal Medicine II, Medical University of 
      Vienna, Waehringer Guertel 18-20, 1090, Vienna, Austria.
FAU - Panzenbock, Adelheid
AU  - Panzenbock A
AD  - Division of Cardiology, Department of Internal Medicine II, Medical University of 
      Vienna, Waehringer Guertel 18-20, 1090, Vienna, Austria.
FAU - Ondracek, Anna S
AU  - Ondracek AS
AD  - Division of Cardiology, Department of Internal Medicine II, Medical University of 
      Vienna, Waehringer Guertel 18-20, 1090, Vienna, Austria.
FAU - Muller, Julian
AU  - Muller J
AD  - Division of Cardiology, Department of Internal Medicine II, Medical University of 
      Vienna, Waehringer Guertel 18-20, 1090, Vienna, Austria.
FAU - Schneider, Matthias
AU  - Schneider M
AD  - Division of Cardiology, Department of Internal Medicine II, Medical University of 
      Vienna, Waehringer Guertel 18-20, 1090, Vienna, Austria.
FAU - Binder, Thomas
AU  - Binder T
AD  - Division of Cardiology, Department of Internal Medicine II, Medical University of 
      Vienna, Waehringer Guertel 18-20, 1090, Vienna, Austria.
FAU - Hell, Lena
AU  - Hell L
AD  - Division of Haematology and Haemostaseology, Department of Internal Medicine I, 
      Medical University of Vienna, Waehringer Guertel 18-20, 1090, Vienna, Austria.
FAU - Lang, Irene M
AU  - Lang IM
AD  - Division of Cardiology, Department of Internal Medicine II, Medical University of 
      Vienna, Waehringer Guertel 18-20, 1090, Vienna, Austria. 
      irene.lang@meduniwien.ac.at.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190716
PL  - Germany
TA  - Basic Res Cardiol
JT  - Basic research in cardiology
JID - 0360342
SB  - IM
MH  - Adult
MH  - Aged
MH  - *Extracellular Traps
MH  - Female
MH  - Fibroblasts/*pathology
MH  - Fibrosis/physiopathology
MH  - Humans
MH  - Leukocytes/*pathology
MH  - Male
MH  - Middle Aged
MH  - ST Elevation Myocardial Infarction/immunology/pathology/*physiopathology
MH  - Ventricular Remodeling/*physiology
PMC - PMC6647191
OTO - NOTNLM
OT  - Fibrocytes
OT  - Neutrophil extracellular traps
OT  - ST-segment elevation myocardial infarction
OT  - Thrombosis
COIS- None declared.
EDAT- 2019/07/18 06:00
MHDA- 2020/02/11 06:00
PMCR- 2019/07/16
CRDT- 2019/07/18 06:00
PHST- 2019/03/24 00:00 [received]
PHST- 2019/06/26 00:00 [accepted]
PHST- 2019/07/18 06:00 [entrez]
PHST- 2019/07/18 06:00 [pubmed]
PHST- 2020/02/11 06:00 [medline]
PHST- 2019/07/16 00:00 [pmc-release]
AID - 10.1007/s00395-019-0740-3 [pii]
AID - 740 [pii]
AID - 10.1007/s00395-019-0740-3 [doi]
PST - epublish
SO  - Basic Res Cardiol. 2019 Jul 16;114(5):33. doi: 10.1007/s00395-019-0740-3.