PMID- 31313795
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20190725
IS  - 2040-3372 (Electronic)
IS  - 2040-3364 (Linking)
VI  - 11
IP  - 29
DP  - 2019 Aug 7
TI  - Ultrabright dye-loaded spherical polyelectrolyte brushes and their fundamental 
      structure-fluorescence tuning principles.
PG  - 14050-14059
LID - 10.1039/c9nr02168j [doi]
AB  - Ultrabright fluorescent particles (UFPs) have attracted increasing attention 
      because of their outstanding signal amplification functions. However, there is 
      still an urgent demand for designing novel UFPs with new components or structures 
      as the existing ones can not satisfy the practical requirements due to their 
      inherent disadvantages. Here we propose a novel ultrabright fluorescent particle 
      platform by doping dyes of 5-aminofluorescein (5-AF) into silica core-based 
      spherical poly (acrylic acid) brushes (SiO(2)@PAA@5-AF) and discuss their 
      fundamental structure-fluorescence tuning principles. A series of brushes with 
      different polymer chain lengths are successfully synthesized and then loaded with 
      5-AF through chemical binding. The high loading amount, suitable density or 
      distribution, and enhanced quantum yield (QY) of 5-AF due to the amide bond 
      formation with PAA chains on brushes are concluded as the three major reasons for 
      the ultrabrightness of SiO(2)@PAA@5-AF. Therefore, a 2350 +/- 445 times brighter 
      brush particle in comparison to a single quantum dot (QD) is realized, and a 2.1 
      +/- 0.4 times fluorescence improvement of a brush vs. a QD normalized by volume is 
      also achieved when taking the hydrodynamic diameter into consideration ( approximately 300 nm 
      vs.  approximately 30 nm). Moreover, the excellent tolerance stabilities in normally applied 
      environments and outstanding label effects to form 4-plexed encoded beads are 
      demonstrated as well. The results in this work strongly indicate a promising 
      potential of SiO(2)@PAA@5-AF as an ultrabright and stable signal amplification 
      tool for biomedical related sensing, labeling, and biodetection.
FAU - Masoomi, Hajar
AU  - Masoomi H
AUID- ORCID: 0000-0002-5776-6141
AD  - School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, 
      China. wangyao@sjtu.edu.cn xuhong@sjtu.edu.cn.
FAU - Wang, Yao
AU  - Wang Y
AUID- ORCID: 0000-0003-0470-9511
AD  - School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, 
      China. wangyao@sjtu.edu.cn xuhong@sjtu.edu.cn.
FAU - Fang, Xiaoxia
AU  - Fang X
AD  - School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, 
      China. wangyao@sjtu.edu.cn xuhong@sjtu.edu.cn.
FAU - Wang, Peirui
AU  - Wang P
AD  - School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, 
      China. wangyao@sjtu.edu.cn xuhong@sjtu.edu.cn.
FAU - Chen, Cang
AU  - Chen C
AD  - School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, 
      China. wangyao@sjtu.edu.cn xuhong@sjtu.edu.cn.
FAU - Liu, Kai
AU  - Liu K
AD  - School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, 
      China. wangyao@sjtu.edu.cn xuhong@sjtu.edu.cn.
FAU - Gu, Hongchen
AU  - Gu H
AUID- ORCID: 0000-0001-8009-2459
AD  - School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, 
      China. wangyao@sjtu.edu.cn xuhong@sjtu.edu.cn.
FAU - Xu, Hong
AU  - Xu H
AUID- ORCID: 0000-0002-2787-5806
AD  - School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, 
      China. wangyao@sjtu.edu.cn xuhong@sjtu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20190717
PL  - England
TA  - Nanoscale
JT  - Nanoscale
JID - 101525249
SB  - IM
EDAT- 2019/07/18 06:00
MHDA- 2019/07/18 06:01
CRDT- 2019/07/18 06:00
PHST- 2019/07/18 06:00 [pubmed]
PHST- 2019/07/18 06:01 [medline]
PHST- 2019/07/18 06:00 [entrez]
AID - 10.1039/c9nr02168j [doi]
PST - ppublish
SO  - Nanoscale. 2019 Aug 7;11(29):14050-14059. doi: 10.1039/c9nr02168j. Epub 2019 Jul 
      17.