PMID- 31316353
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1662-5102 (Print)
IS  - 1662-5102 (Electronic)
IS  - 1662-5102 (Linking)
VI  - 13
DP  - 2019
TI  - A Nitric Oxide-Dependent Presynaptic LTP at Glutamatergic Synapses of the PVN 
      Magnocellular Neurosecretory Cells in vitro in Rats.
PG  - 283
LID - 10.3389/fncel.2019.00283 [doi]
LID - 283
AB  - The magnocellular neurosecretory cells (MNCs) of the hypothalamic paraventricular 
      nucleus (PVN) integrate incoming signals to secrete oxytocin (OT), and 
      vasopressin (VP) from their nerve terminals in the posterior pituitary gland. In 
      the absence of gamma-aminobutyric acid A (GABA(A)) and cannabinoids 1 (CB1) 
      receptor activity, we used whole-cell patch-clamp recording, single-cell reverse 
      transcription-multiplex polymerase chain reaction (SC-RT-mPCR), biocytin 
      histochemistry and pharmacological methods to examine the mechanism of high 
      frequency stimulus (HFS, 100 Hz)-induced long-term potentiation (LTP) at 
      glutamatergic synapses in the PVN MNCs of juvenile male rats. Our results showed 
      that HFS-induced LTP at glutamatergic synapses was accompanied by a decrease in 
      the paired-pulse ratio (PPR) of the PVN MNCs. In these MNCs, HFS-induced LTP 
      persisted in the presence of a group 1 metabotropic glutamate receptor (mGluR1) 
      antagonist; however, it was abolished by an N-methyl-D-aspartic acid (NMDA) 
      receptor blocker. Notably, HFS-induced LTP in the PVN MNCs was completely 
      prevented by a nitric oxide synthase (NOS) inhibitor. The application of an NO 
      donor not only induced the LTP of excitatory glutamatergic inputs in the PVN 
      MNCs, but also occluded the HFS-induced LTP in these MNCs. Moreover, HFS-induced 
      LTP in the PVN MNCs was also abolished by a specific protein kinase A (PKA) 
      inhibitor, KT5720. SC-RT-mPCR analysis revealed that 64.5% (62/96) of MNCs 
      expressed OT mRNA. Our results indicate that a HFS can induce an NMDA receptor 
      and NO cascades dependent on presynaptic glutamatergic LTP in the PVN MNCs via a 
      PKA signaling pathway.
FAU - Zhang, Bin-Bin
AU  - Zhang BB
AD  - Key Laboratory of Cellular Function and Pharmacology of Jilin Province, Yanbian 
      University, Yanji, China.
AD  - Department of Physiology and Pathophysiology, College of Medicine, Yanbian 
      University, Yanji, China.
FAU - Jin, Hua
AU  - Jin H
AD  - Key Laboratory of Cellular Function and Pharmacology of Jilin Province, Yanbian 
      University, Yanji, China.
AD  - Department of Nephrology, Affiliated Hospital of Yanbian University, Yanji, 
      China.
FAU - Bing, Yan-Hua
AU  - Bing YH
AD  - Key Laboratory of Cellular Function and Pharmacology of Jilin Province, Yanbian 
      University, Yanji, China.
AD  - Department of Physiology and Pathophysiology, College of Medicine, Yanbian 
      University, Yanji, China.
FAU - Zhang, Xin-Yuan
AU  - Zhang XY
AD  - Department of Physiology and Pathophysiology, College of Medicine, Yanbian 
      University, Yanji, China.
FAU - Chu, Chun-Ping
AU  - Chu CP
AD  - Key Laboratory of Cellular Function and Pharmacology of Jilin Province, Yanbian 
      University, Yanji, China.
FAU - Li, Yu-Zi
AU  - Li YZ
AD  - Key Laboratory of Cellular Function and Pharmacology of Jilin Province, Yanbian 
      University, Yanji, China.
AD  - Department of Cardiology, Affiliated Hospital of Yanbian University, Yanji, 
      China.
FAU - Qiu, De-Lai
AU  - Qiu DL
AD  - Key Laboratory of Cellular Function and Pharmacology of Jilin Province, Yanbian 
      University, Yanji, China.
AD  - Department of Physiology and Pathophysiology, College of Medicine, Yanbian 
      University, Yanji, China.
LA  - eng
PT  - Journal Article
DEP - 20190627
PL  - Switzerland
TA  - Front Cell Neurosci
JT  - Frontiers in cellular neuroscience
JID - 101477935
PMC - PMC6610542
OTO - NOTNLM
OT  - NMDA receptor
OT  - hypothalamic paraventricular nucleus
OT  - long-term synaptic plasticity
OT  - nitric oxide
OT  - protein kinase A
OT  - whole-cell recording
EDAT- 2019/07/19 06:00
MHDA- 2019/07/19 06:01
PMCR- 2019/01/01
CRDT- 2019/07/19 06:00
PHST- 2019/01/16 00:00 [received]
PHST- 2019/06/11 00:00 [accepted]
PHST- 2019/07/19 06:00 [entrez]
PHST- 2019/07/19 06:00 [pubmed]
PHST- 2019/07/19 06:01 [medline]
PHST- 2019/01/01 00:00 [pmc-release]
AID - 10.3389/fncel.2019.00283 [doi]
PST - epublish
SO  - Front Cell Neurosci. 2019 Jun 27;13:283. doi: 10.3389/fncel.2019.00283. 
      eCollection 2019.