PMID- 31316504
OWN - NLM
STAT- MEDLINE
DCOM- 20201021
LR  - 20201021
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 10
DP  - 2019
TI  - Human Oral Epithelial Cells Impair Bacteria-Mediated Maturation of Dendritic 
      Cells and Render T Cells Unresponsive to Stimulation.
PG  - 1434
LID - 10.3389/fimmu.2019.01434 [doi]
LID - 1434
AB  - The oral mucosa is a first line of defense against pathogenic organisms and yet 
      tolerates food antigens and resident bacteria. Mucosal epithelial cells are 
      emerging as important regulators of innate and adaptive immune responses. 
      However, the contribution of oral epithelial cells (OECs) determining oral 
      immunity is understudied. Here, we evaluated the ability of H413 and TR146 cells, 
      two OEC lines derived from human oral squamous cell carcinomas, and primary OECs 
      to modulate immune responses to a cocktail of Gram(+) and Gram(-) bacteria known 
      as MV130. OECs expressed CD40 constitutively and class II major 
      histocompatibility complex (MHC II) molecules when stimulated with IFNgamma, but not 
      CD80 or CD86. Dendritic cells (DCs) treated with bacteria in co-culture with OECs 
      did not fully mature, as judged by the expression of MHC II, CD80 and CD86, and 
      barely released IL-12 and TNFalpha, compared to control DCs. Furthermore, in the 
      presence of OECs, DCs were unable to stimulate allogenic naive CD4 T cells to 
      produce IFNgamma and TNFalpha. Similarly, OECs in culture with total CD4 T cells or Th1 
      cells stimulated with anti-CD3 and anti-CD28 antibodies abrogated CD25 and CD69 
      expression, T cell proliferation and the release of IFNgamma and TNFalpha. The inhibition 
      on T cell activation by OECs was cell-contact dependent, TGFbeta independent and 
      largely irreversible. Overall, this behavior of OECs is likely key to avoid 
      immune system over-reaction against resident bacteria.
FAU - Molero-Abraham, Magdalena
AU  - Molero-Abraham M
AD  - Department of Immunology, School of Medicine, Complutense University of Madrid, 
      Madrid, Spain.
FAU - Sanchez-Trincado, Jose L
AU  - Sanchez-Trincado JL
AD  - Department of Immunology, School of Medicine, Complutense University of Madrid, 
      Madrid, Spain.
FAU - Gomez-Perosanz, Marta
AU  - Gomez-Perosanz M
AD  - Department of Immunology, School of Medicine, Complutense University of Madrid, 
      Madrid, Spain.
FAU - Torres-Gomez, Alvaro
AU  - Torres-Gomez A
AD  - Department of Immunology, School of Medicine, Complutense University of Madrid, 
      Madrid, Spain.
FAU - Subiza, Jose Luis
AU  - Subiza JL
AD  - Inmunotek SL, Madrid, Spain.
FAU - Lafuente, Esther M
AU  - Lafuente EM
AD  - Department of Immunology, School of Medicine, Complutense University of Madrid, 
      Madrid, Spain.
FAU - Reche, Pedro A
AU  - Reche PA
AD  - Department of Immunology, School of Medicine, Complutense University of Madrid, 
      Madrid, Spain.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190628
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
RN  - 0 (Cytokines)
SB  - IM
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Cytokines/immunology
MH  - Dendritic Cells/*immunology
MH  - Epithelial Cells/*immunology/*microbiology
MH  - *Gram-Negative Bacteria
MH  - *Gram-Positive Bacteria
MH  - Humans
MH  - Mouth Mucosa/*cytology/immunology
PMC - PMC6611079
OTO - NOTNLM
OT  - T cells
OT  - bacteria
OT  - dendritic cells
OT  - immunomodulation
OT  - oral epithelial cells
EDAT- 2019/07/19 06:00
MHDA- 2020/10/22 06:00
PMCR- 2019/01/01
CRDT- 2019/07/19 06:00
PHST- 2019/03/22 00:00 [received]
PHST- 2019/06/07 00:00 [accepted]
PHST- 2019/07/19 06:00 [entrez]
PHST- 2019/07/19 06:00 [pubmed]
PHST- 2020/10/22 06:00 [medline]
PHST- 2019/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2019.01434 [doi]
PST - epublish
SO  - Front Immunol. 2019 Jun 28;10:1434. doi: 10.3389/fimmu.2019.01434. eCollection 
      2019.