PMID- 31316581 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20220409 IS - 1746-4811 (Print) IS - 1746-4811 (Electronic) IS - 1746-4811 (Linking) VI - 15 DP - 2019 TI - Development of an oligonucleotide dye solution facilitates high throughput and cost-efficient chromosome identification in peanut. PG - 69 LID - 10.1186/s13007-019-0451-7 [doi] LID - 69 AB - BACKGROUND: Development of oligonucleotide probes facilitates chromosome identification via fluorescence in situ hybridization (FISH) in many organisms. RESULTS: We report a high throughput and economical method of chromosome identification based on the development of a dye solution containing 2 x saline-sodium citrate (SSC) and oligonucleotide probes. Based on the concentration, staining time, and sequence effects of oligonucleotides, an efficient probe dye of peanut was developed for chromosome identification. To validate the effects of this solution, 200 slides derived from 21 accessions of the cultivated peanut and 30 wild Arachis species were painted to identify Arachis genomes and establish karyotypes. The results showed that one jar of dye could be used to paint 10 chromosome preparations and recycled at least 10 times to efficiently dye more than 100 slides. The A, B, K, F, E, and H genomes showed unique staining karyotype patterns and signal colors. CONCLUSIONS: Based on the karyotype patterns of Arachis genomes, we revealed the relationships among the A, B, K, F, E, and H genomes in genus Arachis, and demonstrated the potential for adoption of this oligonucleotide dye solution in practice. FAU - Du, Pei AU - Du P AD - Industrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops Improvement, Zhengzhou, 450002 Henan China. FAU - Cui, Caihong AU - Cui C AD - Industrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops Improvement, Zhengzhou, 450002 Henan China. FAU - Liu, Hua AU - Liu H AD - Industrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops Improvement, Zhengzhou, 450002 Henan China. FAU - Fu, Liuyang AU - Fu L AD - 3School of Life Sciences, Zhengzhou University, Zhengzhou, 450001 Henan China. ISNI: 0000 0001 2189 3846. GRID: grid.207374.5 FAU - Li, Lina AU - Li L AD - Industrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops Improvement, Zhengzhou, 450002 Henan China. FAU - Dai, Xiaodong AU - Dai X AD - Industrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops Improvement, Zhengzhou, 450002 Henan China. FAU - Qin, Li AU - Qin L AD - Industrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops Improvement, Zhengzhou, 450002 Henan China. FAU - Wang, Siyu AU - Wang S AD - 3School of Life Sciences, Zhengzhou University, Zhengzhou, 450001 Henan China. ISNI: 0000 0001 2189 3846. GRID: grid.207374.5 FAU - Han, Suoyi AU - Han S AD - Industrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops Improvement, Zhengzhou, 450002 Henan China. FAU - Xu, Jing AU - Xu J AD - Industrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops Improvement, Zhengzhou, 450002 Henan China. FAU - Liu, Bing AU - Liu B AD - Industrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops Improvement, Zhengzhou, 450002 Henan China. FAU - Huang, Bingyan AU - Huang B AD - Industrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops Improvement, Zhengzhou, 450002 Henan China. FAU - Tang, Fengshou AU - Tang F AD - Industrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops Improvement, Zhengzhou, 450002 Henan China. FAU - Dong, Wenzhao AU - Dong W AD - Industrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops Improvement, Zhengzhou, 450002 Henan China. FAU - Qi, Zengjun AU - Qi Z AD - 2State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095 China. ISNI: 0000 0000 9750 7019. GRID: grid.27871.3b FAU - Zhang, Xinyou AU - Zhang X AD - Industrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops Improvement, Zhengzhou, 450002 Henan China. LA - eng PT - Journal Article DEP - 20190708 PL - England TA - Plant Methods JT - Plant methods JID - 101245798 PMC - PMC6613257 OTO - NOTNLM OT - Arachis species OT - FISH OT - Karyotype OT - Oligonucleotide dye solution COIS- Competing interestsThe authors declare that they have no competing interests. EDAT- 2019/07/19 06:00 MHDA- 2019/07/19 06:01 PMCR- 2019/07/08 CRDT- 2019/07/19 06:00 PHST- 2019/04/16 00:00 [received] PHST- 2019/06/20 00:00 [accepted] PHST- 2019/07/19 06:00 [entrez] PHST- 2019/07/19 06:00 [pubmed] PHST- 2019/07/19 06:01 [medline] PHST- 2019/07/08 00:00 [pmc-release] AID - 451 [pii] AID - 10.1186/s13007-019-0451-7 [doi] PST - epublish SO - Plant Methods. 2019 Jul 8;15:69. doi: 10.1186/s13007-019-0451-7. eCollection 2019.