PMID- 31317199
OWN - NLM
STAT- MEDLINE
DCOM- 20210323
LR  - 20210323
IS  - 2737-5935 (Electronic)
IS  - 0037-5675 (Print)
IS  - 0037-5675 (Linking)
VI  - 61
IP  - 4
DP  - 2020 Apr
TI  - Generation of IgE-specific cytotoxic T lymphocytes as a novel immunotherapeutic 
      approach for the treatment of allergic asthma.
PG  - 212-220
LID - 10.11622/smedj.2019068 [doi]
AB  - INTRODUCTION: Overproduction of immunoglobulin E (IgE) by a subset of B cells 
      plays a key role in the pathogenesis of allergic asthma. Anti-IgE monoclonal 
      antibodies have been successfully used to treat the disease, but long-term 
      application is required. METHODS: For this study, cytotoxic T lymphocytes (CTLs) 
      against IgE-producing B cells were generated ex vivo by stimulating naive CD8 T 
      cells with IgE-derived peptides presented by Drosophila-derived artificial 
      antigen-presenting cells. Based on the treatment of allergic asthma in mice, the 
      inhibitive effect of this CTL on IgE responses and airway inflammation was 
      determined with the enzyme-linked immunosorbent assay and histochemical method. 
      RESULTS: The IgE-specific CTLs effectively lysed target cells in vitro, while the 
      adoptively transferred CTLs specifically inhibited IgE responses and airway 
      inflammation in an asthmatic mouse model. The effect of IgE-specific CTLs is MHC 
      (major histocompatibility complex) Class I-restricted and requires the expression 
      of perforin. CONCLUSION: IgE-specific CTLs generated ex vivo may provide a novel 
      treatment for allergic asthma and lead to a new therapy for other immunological 
      disorders.
CI  - Copyright: (c) Singapore Medical Association.
FAU - Cui, Kangle
AU  - Cui K
AD  - College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 
      China.
FAU - Chen, Yanna
AU  - Chen Y
AD  - College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 
      China.
FAU - Zhou, Li
AU  - Zhou L
AD  - College Hospital, Zhejiang Sci-Tech University, Hangzhou, China.
FAU - Yang, Chunxia
AU  - Yang C
AD  - Shanghai Yuyan Cell Research Company, Shanghai, China.
FAU - Du, Yao
AU  - Du Y
AD  - College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 
      China.
FAU - Shi, Weixing
AU  - Shi W
AD  - College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 
      China.
AD  - Johnson and Johnson Pharmaceutical Research and Development LLC, San Diego, CA, 
      USA.
FAU - Cai, Zeling
AU  - Cai Z
AD  - Johnson and Johnson Pharmaceutical Research and Development LLC, San Diego, CA, 
      USA.
FAU - Chen, Jian
AU  - Chen J
AD  - College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20190711
PL  - India
TA  - Singapore Med J
JT  - Singapore medical journal
JID - 0404516
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Asthma/*therapy
MH  - Disease Models, Animal
MH  - Hypersensitivity
MH  - Immunoglobulin E/*immunology
MH  - Immunotherapy/*methods
MH  - In Vitro Techniques
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - T-Lymphocytes, Cytotoxic/*immunology
PMC - PMC7905137
OTO - NOTNLM
OT  - artificial antigen-presenting cells
OT  - cytotoxic T lymphocytes
OT  - immunoglobulin E
OT  - perforin
EDAT- 2019/07/19 06:00
MHDA- 2021/03/24 06:00
PMCR- 2020/04/01
CRDT- 2019/07/19 06:00
PHST- 2019/07/19 06:00 [pubmed]
PHST- 2021/03/24 06:00 [medline]
PHST- 2019/07/19 06:00 [entrez]
PHST- 2020/04/01 00:00 [pmc-release]
AID - j61/4/212 [pii]
AID - SMJ-61-212 [pii]
AID - 10.11622/smedj.2019068 [doi]
PST - ppublish
SO  - Singapore Med J. 2020 Apr;61(4):212-220. doi: 10.11622/smedj.2019068. Epub 2019 
      Jul 11.