PMID- 31318411
OWN - NLM
STAT- MEDLINE
DCOM- 20200902
LR  - 20200902
IS  - 1529-7268 (Electronic)
IS  - 0006-3363 (Linking)
VI  - 101
IP  - 6
DP  - 2019 Dec 24
TI  - Sperm chromosomal abnormalities and their contribution to human embryo 
      aneuploidy.
PG  - 1091-1101
LID - 10.1093/biolre/ioz125 [doi]
AB  - In this work we reviewed 18 years of experience using fluorescence in situ 
      hybridization (FISH) for sperm aneuploidy testing. We evaluated parameters 
      associated with increased numerical sperm chromosome abnormalities and determined 
      the male contribution to embryo aneploidies in terms of reproductive outcome by 
      increased sperm aneuploidy. This retrospective study analyzed data from 2008 
      sperm samples of infertile males undergoing FISH analysis because of clinical 
      history of repetitive implantation failure, recurrent miscarriage, impaired sperm 
      parameters, or mixed causes. Sperm concentration was the only sperm parameter 
      associated with FISH results-we observed a gradual increase of abnormal sperm 
      FISH results in males with decreasing sperm concentration. However, a great 
      proportion of normozoospermic males also showed increased sperm aneuploidies, 
      suggesting that sperm parameters alone do not enable identification of a 
      substantial proportion of infertile males at risk of sperm aneuploidies. 
      Regarding reproductive outcomes, couples with normal sperm FISH results for the 
      male had similar outcomes regardless of conventional in vitro fertilization 
      (IVF), intracytoplasmic sperm injection (ICSI), or preimplantation genetic 
      testing for aneuploidies (PGT-A). However, couples with abnormal sperm FISH 
      results for the male showed better clinical outcomes after PGT-A, suggesting a 
      potential contribution of sperm to embryo aneuploidy. Moreover, PGT-A cycles 
      showed better clinical outcomes when 24 chromosomes were analyzed by array 
      comparative genome hybridization (aCGH) or next-generation sequencing (NGS) 
      instead of only nine chromosomes analyzed by FISH. In conclusion, sperm FISH 
      analysis offers clinical prognostic value to evaluate reproductive possibilities 
      in infertile couples. Therefore, couples with abnormal sperm FISH results should 
      be offered genetic counseling and presented with clinical options such as PGT-A.
CI  - (c) The Author(s) 2019. Published by Oxford University Press on behalf of Society 
      for the Study of Reproduction. All rights reserved. For permissions, please 
      e-mail: journals.permissions@oup.com.
FAU - Rodrigo, Lorena
AU  - Rodrigo L
AD  - Igenomix, Valencia, Spain.
FAU - Meseguer, Marcos
AU  - Meseguer M
AD  - IVIRMA Valencia, Valencia, Spain.
FAU - Mateu, Emilia
AU  - Mateu E
AD  - Igenomix, Valencia, Spain.
FAU - Mercader, Amparo
AU  - Mercader A
AD  - IVIRMA Valencia, Valencia, Spain.
FAU - Peinado, Vanessa
AU  - Peinado V
AD  - Igenomix, Valencia, Spain.
FAU - Bori, Lorena
AU  - Bori L
AD  - IVIRMA Valencia, Valencia, Spain.
FAU - Campos-Galindo, Inmaculada
AU  - Campos-Galindo I
AD  - Igenomix, Valencia, Spain.
FAU - Milan, Miguel
AU  - Milan M
AD  - Igenomix, Valencia, Spain.
FAU - Garcia-Herrero, Sandra
AU  - Garcia-Herrero S
AD  - Igenomix, Valencia, Spain.
FAU - Simon, Carlos
AU  - Simon C
AD  - Igenomix Foundation/Instituto de Investigacion Sanitaria Hospital Clinico, 
      Valencia, Spain.
AD  - Department of Pediatrics, Obstetrics and Gynecology, University of Valencia, 
      Valencia, Spain.
FAU - Rubio, Carmen
AU  - Rubio C
AD  - Igenomix, Valencia, Spain.
LA  - eng
PT  - Journal Article
PT  - Review
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
SB  - IM
MH  - *Aneuploidy
MH  - Chromosome Aberrations/*embryology
MH  - Comparative Genomic Hybridization
MH  - Female
MH  - Fertilization in Vitro
MH  - High-Throughput Nucleotide Sequencing
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infertility, Male/genetics/therapy
MH  - Male
MH  - Oligospermia/genetics
MH  - Precision Medicine
MH  - Pregnancy
MH  - *Preimplantation Diagnosis
MH  - Retrospective Studies
MH  - Sperm Count
MH  - Sperm Injections, Intracytoplasmic
MH  - Sperm Motility
MH  - Spermatozoa/*abnormalities/ultrastructure
OTO - NOTNLM
OT  - aneuploidy
OT  - blastocyst
OT  - implantation
OT  - in vitro fertilization (IVF)
OT  - intracytoplasmic sperm injection (ICSI)
OT  - male infertility
OT  - pregnancy
OT  - preimplantation embryo
OT  - sperm
EDAT- 2019/07/19 06:00
MHDA- 2020/09/04 06:00
CRDT- 2019/07/19 06:00
PHST- 2018/09/20 00:00 [received]
PHST- 2019/01/30 00:00 [revised]
PHST- 2019/07/16 00:00 [accepted]
PHST- 2019/07/19 06:00 [pubmed]
PHST- 2020/09/04 06:00 [medline]
PHST- 2019/07/19 06:00 [entrez]
AID - 5535530 [pii]
AID - 10.1093/biolre/ioz125 [doi]
PST - ppublish
SO  - Biol Reprod. 2019 Dec 24;101(6):1091-1101. doi: 10.1093/biolre/ioz125.