PMID- 31321607
OWN - NLM
STAT- MEDLINE
DCOM- 20200514
LR  - 20200522
IS  - 1573-6849 (Electronic)
IS  - 0967-3849 (Linking)
VI  - 27
IP  - 4
DP  - 2019 Dec
TI  - Identification of passion fruit (Passiflora edulis) chromosomes using BAC-FISH.
PG  - 299-311
LID - 10.1007/s10577-019-09614-0 [doi]
AB  - Passiflora edulis, the yellow passion fruit, is the main crop from the Passiflora 
      genus, which comprises 525 species with its diversity center in South America. 
      Genetic maps and a BAC (bacterial artificial chromosome) genomic library are 
      available, but the nine chromosome pairs of similar size and morphology (2n = 18) 
      hamper chromosome identification, leading to different proposed karyotypes. Thus, 
      the aim of this study was to establish chromosome-specific markers for the yellow 
      passion fruit using single-copy and repetitive sequences as probes in fluorescent 
      in situ hybridizations (FISH) to allow chromosome identification and future 
      integration with whole genome data. Thirty-six BAC clones harboring genes and 
      three retrotransposons (Ty1-copy, Ty3-gypsy, and LINE) were selected. Twelve BACs 
      exhibited a dispersed pattern similar to that revealed by retroelements, and one 
      exhibited subtelomeric distribution. Twelve clones showed unique signals in 
      terminal or subterminal regions of the chromosomes, allowing their genes to be 
      anchored to six chromosome pairs that can be identified with single-copy markers. 
      The markers developed herein will provide an important tool for genomic and 
      evolutionary studies in the Passiflora genus.
FAU - Sader, M A
AU  - Sader MA
AD  - Department of Botany, Federal University of Pernambuco, Recife, Brazil.
FAU - Dias, Y
AU  - Dias Y
AD  - Department of Botany, Federal University of Pernambuco, Recife, Brazil.
FAU - Costa, Z P
AU  - Costa ZP
AD  - Department of Genetics, "Luiz de Queiroz" College of Agriculture, University of 
      Sao Paulo, Piracicaba, Brazil.
FAU - Munhoz, C
AU  - Munhoz C
AD  - Department of Genetics, "Luiz de Queiroz" College of Agriculture, University of 
      Sao Paulo, Piracicaba, Brazil.
FAU - Penha, H
AU  - Penha H
AD  - Department of Technology, Faculty of Agricultural and Veterinary Sciences, Sao 
      Paulo State University (UNESP), Jaboticabal, Brazil.
FAU - Berges, H
AU  - Berges H
AD  - French Plant Genomic Resources Center (CNRGV)/ INRA, Toulouse, France.
FAU - Vieira, M L C
AU  - Vieira MLC
AD  - Department of Genetics, "Luiz de Queiroz" College of Agriculture, University of 
      Sao Paulo, Piracicaba, Brazil.
FAU - Pedrosa-Harand, Andrea
AU  - Pedrosa-Harand A
AUID- ORCID: 0000-0001-5213-4770
AD  - Department of Botany, Federal University of Pernambuco, Recife, Brazil. 
      andrea.harand@ufpe.br.
AD  - Laboratorio de Citogenetica e Evolucao Vegetal, Departamento de Botanica, Centro 
      de Biociencias, Universidade Federal de Pernambuco, R. Prof. Moraes Rego, s/n, 
      CDU, Recife, PE, 50670-901, Brazil. andrea.harand@ufpe.br.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190718
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
RN  - 0 (Genetic Markers)
RN  - 0 (Retroelements)
SB  - IM
EIN - Chromosome Res. 2021 Jun;29(2):237-238. PMID: 32430859
MH  - Chromosome Mapping
MH  - *Chromosomes, Plant
MH  - *Genetic Markers
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotype
MH  - Passiflora/*genetics
MH  - Repetitive Sequences, Nucleic Acid
MH  - Retroelements
OTO - NOTNLM
OT  - Bacterial artificial chromosome (BAC)
OT  - Cytogenetic map
OT  - Fluorescent in situ hybridization (FISH)
OT  - Karyotype
OT  - Repetitive DNA sequences
OT  - Retrotransposon
EDAT- 2019/07/20 06:00
MHDA- 2020/05/15 06:00
CRDT- 2019/07/20 06:00
PHST- 2019/04/12 00:00 [received]
PHST- 2019/07/05 00:00 [accepted]
PHST- 2019/06/13 00:00 [revised]
PHST- 2019/07/20 06:00 [pubmed]
PHST- 2020/05/15 06:00 [medline]
PHST- 2019/07/20 06:00 [entrez]
AID - 10.1007/s10577-019-09614-0 [pii]
AID - 10.1007/s10577-019-09614-0 [doi]
PST - ppublish
SO  - Chromosome Res. 2019 Dec;27(4):299-311. doi: 10.1007/s10577-019-09614-0. Epub 
      2019 Jul 18.