PMID- 31322296
OWN - NLM
STAT- MEDLINE
DCOM- 20200630
LR  - 20200630
IS  - 1600-0722 (Electronic)
IS  - 0909-8836 (Linking)
VI  - 127
IP  - 6
DP  - 2019 Dec
TI  - Effects of ionizing irradiation and interface backscatter on human mesenchymal 
      stem cells cultured on titanium surfaces.
PG  - 500-507
LID - 10.1111/eos.12654 [doi]
AB  - Radiotherapy to the head and neck region negatively influences the 
      osseointegration and survival of dental implants. The effects of cobalt 60 ((60) 
      Co) ionizing radiation and the impact of backscatter rays were investigated on 
      human mesenchymal stem cells cultured on titanium surfaces. Bone marrow-derived 
      human mesenchymal stem cells were seeded on titanium (Ti), fluoride-modified 
      titanium (TiF), and tissue culture plastic. Cells were exposed to ionizing 
      gamma-radiation in single doses of 2, 6, or 10 Gy using a (60) Co source. Density and 
      distribution of cells were evaluated using confocal laser-scanning microscopy, 
      21 d post-irradiation. Lactate dehydrogenase concentration and the levels of 
      total protein and cytokines/chemokines were measured in the cell-culture medium 
      on days 1, 3, 7, 14, and 21 post-irradiation. Unirradiated cells were used as the 
      control. Irradiation had no effect on cell viability, collagen and actin 
      expression, or cell distribution, but induced an initial increase in the 
      secretion of interleukin (IL)-6, IL-8, monocyte chemotactic protein 1 (MCP-1), 
      and vascular endothelial growth factor (VEGF), followed by a decrease in 
      secretion after 3 or 7 d. Irradiation resulted in secretion of a lower amount of 
      all analytes examined compared with controls on day 21, irrespective of radiation 
      dose and growth surface. Backscattering from titanium did not influence the cell 
      response significantly, suggesting a clinical potential for achieving successful 
      osseointegration of dental implants placed before radiotherapy.
CI  - (c) 2019 The Authors. Eur J Oral Sci published by John Wiley & Sons Ltd.
FAU - Printzell, Lisa
AU  - Printzell L
AUID- ORCID: 0000-0001-7134-1484
AD  - Department of Prosthodontics, Faculty for Dentistry, Institute of Clinical 
      Dentistry, University of Oslo, Oslo, Norway.
FAU - Reseland, Janne E
AU  - Reseland JE
AD  - Department of Biomaterials, Faculty for Dentistry, Institute of Clinical 
      Dentistry, University of Oslo, Oslo, Norway.
FAU - Edin, Nina F J
AU  - Edin NFJ
AD  - Department of Physics, Faculty of Mathematics and Natural Science, University of 
      Oslo, Oslo, Norway.
FAU - Ellingsen, Jan E
AU  - Ellingsen JE
AD  - Department of Prosthodontics, Faculty for Dentistry, Institute of Clinical 
      Dentistry, University of Oslo, Oslo, Norway.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190719
PL  - England
TA  - Eur J Oral Sci
JT  - European journal of oral sciences
JID - 9504563
RN  - 0 (CCL2 protein, human)
RN  - 0 (CXCL8 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Dental Implants)
RN  - 0 (IL6 protein, human)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
RN  - 0 (VEGFA protein, human)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - D1JT611TNE (Titanium)
SB  - IM
MH  - Cells, Cultured
MH  - Chemokine CCL2/metabolism
MH  - *Dental Implants
MH  - Humans
MH  - Interleukin-6/metabolism
MH  - Interleukin-8/metabolism
MH  - Mesenchymal Stem Cells/*radiation effects
MH  - *Osseointegration
MH  - *Radiation, Ionizing
MH  - Surface Properties
MH  - *Titanium
MH  - Vascular Endothelial Growth Factor A/metabolism
OTO - NOTNLM
OT  - dental implants
OT  - head and neck cancer
OT  - oral rehabilitation
OT  - primary placement
OT  - radiotherapy
EDAT- 2019/07/20 06:00
MHDA- 2020/07/01 06:00
CRDT- 2019/07/20 06:00
PHST- 2019/06/14 00:00 [accepted]
PHST- 2019/07/20 06:00 [pubmed]
PHST- 2020/07/01 06:00 [medline]
PHST- 2019/07/20 06:00 [entrez]
AID - 10.1111/eos.12654 [doi]
PST - ppublish
SO  - Eur J Oral Sci. 2019 Dec;127(6):500-507. doi: 10.1111/eos.12654. Epub 2019 Jul 
      19.