PMID- 31323001
OWN - NLM
STAT- MEDLINE
DCOM- 20200917
LR  - 20210213
IS  - 1437-4331 (Electronic)
IS  - 1434-6621 (Linking)
VI  - 57
IP  - 12
DP  - 2019 Nov 26
TI  - EGFR and EGFR ligands in serum in healthy women; reference intervals and age 
      dependency.
PG  - 1948-1955
LID - 10.1515/cclm-2019-0376 [doi]
AB  - Background The epidermal growth factor receptor (EGFR) system is involved in 
      cancer pathogenesis and serves as an important target for multiple cancer 
      treatments. EGFR and its ligands epidermal growth factor (EGF), heparin-binding 
      epidermal growth factor (HB-EGF), betacellulin (BTC), amphiregulin (AREG) and 
      transforming growth factor alpha (TGF-alpha) have potential applications as prognostic or 
      predictive serological biomarkers in cancer. The aim was to establish EGFR and 
      EGFR ligand reference intervals in healthy women. Methods EGFR and EGFR ligands 
      were measured in serum from 419 healthy women aged 26-78 years. The need for age 
      partitioned reference intervals was evaluated using Lahti's method. EGFR and EGF 
      were analyzed using ELISA assays, whereas HB-EGF, BTC, AREG and TGF-alpha were 
      analyzed using the highly sensitive automated single molecule array (Simoa) 
      enabling detection below the lower reference limit for all six biomarkers. 
      Results Reference intervals for EGFR and the EGFR ligands were determined as the 
      2.5th and 97.5th percentiles. All six biomarkers were detectable in all serum 
      samples. For EGFR, EGF, HB-EGF and TGF-alpha, reference intervals were established 
      for women <55 years and for women >55 years, whilst common reference intervals 
      were established for AREG and BTC including women aged 26-78 years. Conclusions 
      Age specific reference intervals were determined for EGFR, EGF, HB-EGF, BTC, AREG 
      and TGF-alpha.
FAU - Kjaer, Ina Mathilde
AU  - Kjaer IM
AUID- ORCID: 0000-0002-6351-379X
AD  - Department of Biochemistry and Immunology, Lillebaelt Hospital, University 
      Hospital of Southern Denmark, Vejle, Denmark.
AD  - Department of Regional Health Research, Faculty of Health Sciences, University of 
      Southern Denmark, Odense, Denmark.
FAU - Olsen, Dorte Aalund
AU  - Olsen DA
AD  - Department of Biochemistry and Immunology, Lillebaelt Hospital, University 
      Hospital of Southern Denmark, Vejle, Denmark.
FAU - Alnor, Anne
AU  - Alnor A
AUID- ORCID: 0000-0002-6131-8886
AD  - Department of Biochemistry and Immunology, Lillebaelt Hospital, University 
      Hospital of Southern Denmark, Vejle, Denmark.
FAU - Brandslund, Ivan
AU  - Brandslund I
AUID- ORCID: 0000-0002-4203-5442
AD  - Department of Biochemistry and Immunology, Lillebaelt Hospital, University 
      Hospital of Southern Denmark, Vejle, Denmark.
AD  - Department of Regional Health Research, Faculty of Health Sciences, University of 
      Southern Denmark, Odense, Denmark.
FAU - Bechmann, Troels
AU  - Bechmann T
AUID- ORCID: 0000-0001-6838-6745
AD  - Department of Regional Health Research, Faculty of Health Sciences, University of 
      Southern Denmark, Odense, Denmark.
AD  - Department of Oncology, Lillebaelt Hospital, University Hospital of Southern 
      Denmark, Vejle, Denmark.
FAU - Madsen, Jonna Skov
AU  - Madsen JS
AUID- ORCID: 0000-0001-6668-4714
AD  - Department of Biochemistry and Immunology, Lillebaelt Hospital, University 
      Hospital of Southern Denmark, Vejle, Denmark.
AD  - Department of Regional Health Research, Faculty of Health Sciences, University of 
      Southern Denmark, Odense, Denmark.
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Clin Chem Lab Med
JT  - Clinical chemistry and laboratory medicine
JID - 9806306
RN  - 0 (Amphiregulin)
RN  - 0 (Betacellulin)
RN  - 0 (Biomarkers)
RN  - 0 (EGF Family of Proteins)
RN  - 0 (Heparin-binding EGF-like Growth Factor)
RN  - 0 (Ligands)
RN  - 0 (Transforming Growth Factor alpha)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - EC 2.7.10.1 (EGFR protein, human)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Amphiregulin/analysis/blood
MH  - Betacellulin/analysis/blood
MH  - Biomarkers/blood
MH  - EGF Family of Proteins/*analysis/blood
MH  - Epidermal Growth Factor/analysis/blood
MH  - ErbB Receptors/analysis/blood
MH  - Female
MH  - Heparin-binding EGF-like Growth Factor/analysis/blood
MH  - Humans
MH  - Ligands
MH  - Middle Aged
MH  - Reference Standards
MH  - Reference Values
MH  - Transforming Growth Factor alpha/analysis/blood
OTO - NOTNLM
OT  - cancer biomarkers
OT  - epidermal growth factor receptor
OT  - epidermal growth factor receptor ligands
OT  - reference interval
OT  - serum
EDAT- 2019/07/20 06:00
MHDA- 2020/09/18 06:00
CRDT- 2019/07/20 06:00
PHST- 2019/04/07 00:00 [received]
PHST- 2019/06/17 00:00 [accepted]
PHST- 2019/07/20 06:00 [pubmed]
PHST- 2020/09/18 06:00 [medline]
PHST- 2019/07/20 06:00 [entrez]
AID - cclm-2019-0376 [pii]
AID - 10.1515/cclm-2019-0376 [doi]
PST - ppublish
SO  - Clin Chem Lab Med. 2019 Nov 26;57(12):1948-1955. doi: 10.1515/cclm-2019-0376.