PMID- 31323329
OWN - NLM
STAT- MEDLINE
DCOM- 20200106
LR  - 20200106
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 93
DP  - 2019 Oct
TI  - A potential probiotic Chromobacterium aquaticum with bacteriocin-like activity 
      enhances the expression of indicator genes associated with nutrient metabolism, 
      growth performance and innate immunity against pathogen infections in zebrafish 
      (Danio rerio).
PG  - 124-134
LID - S1050-4648(19)30751-X [pii]
LID - 10.1016/j.fsi.2019.07.042 [doi]
AB  - The use of probiotics as alternatives to antibiotics for disease control is a 
      relatively eco-friendly approach in aquaculture; hence, studies isolating and 
      assessing the benefit of potential probiotics to fish farming are common. The 
      zebrafish is an excellent model system for validating beneficial functions of 
      potential probiotics before their practical application in aquaculture. Here, a 
      potentially probiotic Chromobacterium aquaticum was isolated from lake water 
      samples and characterized by biochemical analysis and 16S rDNA sequencing. The 
      probiotic produced extracellular enzymes (protease and xylanase) and a 
      bacteriocin-like substance, which exhibited tolerance to extreme pH and 
      high-temperature conditions and broad-spectrum bactericidal activity against 
      diverse pathogens, including aquatic, foodborne, clinical and plant pathogens. 
      The effects of C. aquaticum on zebrafish nutrient metabolism, growth performance 
      and innate immunity were evaluated by measuring the expression of indicator genes 
      after C. aquaticum feeding for 8 weeks. Fish administered the probiotic exhibited 
      significantly increased hepatic mRNA expression of carbohydrate 
      metabolism-related genes, including glucokinase (GK), hexokinase (HK), 
      glucose-6-phosphatase (G6Pase), and pyruvate kinase (PK-L), and growth-related 
      genes, including the growth hormone receptor (GHR) and insulin-like growth 
      factor-1 (IGF-1). Innate immune-related genes (IL-1beta, IL-6, TNF-alpha, IL-10, IL-21, 
      NF-kappab, lysozyme and complement C3b) were induced in fish with probiotic 
      supplementation. Probiotic-treated fish exhibited a higher survival rate than 
      control fish after challenge with Aeromonas hydrophila and Streptococcus iniae. 
      Together, these data suggest that C. aquaticum, as a probiotic feed supplement, 
      could enhance nutrient metabolism and growth performance and could modulate 
      innate immunity against A. hydrophila and S. iniae in zebrafish.
CI  - Copyright (c) 2019 Elsevier Ltd. All rights reserved.
FAU - Yi, Che-Chun
AU  - Yi CC
AD  - Department of Biological Science and Technology, National Pingtung University of 
      Science and Technology, Pingtung, 912, Taiwan.
FAU - Liu, Chun-Hung
AU  - Liu CH
AD  - Department of Aquaculture, National Pingtung University of Science and 
      Technology, Pingtung, 912, Taiwan; Research Center for Animal Biologics, National 
      Pingtung University of Science and Technology, Pingtung, 912, Taiwan.
FAU - Chuang, Kuo-Pin
AU  - Chuang KP
AD  - Graduate Institute of Animal Vaccine Technology, College of Veterinary Medicine, 
      National Pingtung University of Science and Technology, Pingtung, 912, Taiwan; 
      Research Center for Animal Biologics, National Pingtung University of Science and 
      Technology, Pingtung, 912, Taiwan.
FAU - Chang, Yi-Ting
AU  - Chang YT
AD  - Department of Biological Science and Technology, National Pingtung University of 
      Science and Technology, Pingtung, 912, Taiwan.
FAU - Hu, Shao-Yang
AU  - Hu SY
AD  - Department of Biological Science and Technology, National Pingtung University of 
      Science and Technology, Pingtung, 912, Taiwan; Research Center for Animal 
      Biologics, National Pingtung University of Science and Technology, Pingtung, 912, 
      Taiwan. Electronic address: syhu@mail.npust.edu.tw.
LA  - eng
PT  - Journal Article
DEP - 20190716
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Bacteriocins)
SB  - IM
MH  - Aeromonas hydrophila/physiology
MH  - Animal Feed/analysis
MH  - Animals
MH  - Bacteriocins/*pharmacology
MH  - Chromobacterium/*chemistry
MH  - Diet/veterinary
MH  - Dose-Response Relationship, Drug
MH  - Fish Diseases/*immunology
MH  - Gene Expression/*drug effects
MH  - Gram-Negative Bacterial Infections/immunology/veterinary
MH  - Immunity, Innate/*drug effects
MH  - Probiotics/*pharmacology
MH  - Random Allocation
MH  - Streptococcal Infections/immunology/veterinary
MH  - Streptococcus iniae/physiology
MH  - Zebrafish/growth & development/*immunology/metabolism
OTO - NOTNLM
OT  - Bacteriocin
OT  - Chromobacterium aquaticum
OT  - Disease resistance
OT  - Innate immunity
OT  - Zebrafish
EDAT- 2019/07/20 06:00
MHDA- 2020/01/07 06:00
CRDT- 2019/07/20 06:00
PHST- 2019/03/12 00:00 [received]
PHST- 2019/05/19 00:00 [revised]
PHST- 2019/07/16 00:00 [accepted]
PHST- 2019/07/20 06:00 [pubmed]
PHST- 2020/01/07 06:00 [medline]
PHST- 2019/07/20 06:00 [entrez]
AID - S1050-4648(19)30751-X [pii]
AID - 10.1016/j.fsi.2019.07.042 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2019 Oct;93:124-134. doi: 10.1016/j.fsi.2019.07.042. Epub 
      2019 Jul 16.