PMID- 31323558
OWN - NLM
STAT- MEDLINE
DCOM- 20191211
LR  - 20191217
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 1125
DP  - 2019 Sep 1
TI  - Simultaneous determination of eight microcystins in fish by PRiME pass-through 
      cleanup and online solid phase extraction coupled to ultra high performance 
      liquid chromatography-tandem mass spectrometry.
PG  - 121709
LID - S1570-0232(19)30514-8 [pii]
LID - 10.1016/j.jchromb.2019.06.036 [doi]
AB  - A rapid and sensitive method was developed based on online solid phase extraction 
      coupled to ultra high performance liquid chromatography-tandem mass spectrometry 
      (online SPE-UHPLC-MS/MS) for the simultaneous determination of eight common 
      microcystins (MCs) in fish. Analytes, including MC-LR, MC-RR, MC-LY, MC-LW, 
      MC-YR, MC-WR, MC-LF and MC-LA, were extracted by the solvent of MeOH: water 
      (80:20, v/v) with 0.1% FA, and the extracted solution was cleaned up by one-step 
      PRiME (process, robustness, improvements, matrix effects, ease of use) procedure 
      before online SPE-UHPLC-MS/MS analysis. After the optimization of sample 
      pretreatment conditions and instrumental parameters, method validation 
      experiments were carried out to evaluate the performance of the method Good 
      linearities were obtained in the range of 0.5-20 mug/L with r(2) larger than 0.99. 
      The average recoveries of eight MCs spiked at 2, 10 and 20 mug/kg in fish samples 
      were from 70.5% to 98.9% with relative standard deviations (RSDs) 1.3%-3.5% for 
      repeatability and 2.2%-7.3% for reproducibility. Limits of detections (LODs) were 
      ranged from 0.1 to 0.5 mug/kg and Limits of quantifications (LOQs) were in the 
      range of 0.3-1.5 mug/kg. The developed method was further applied to the analysis 
      of fish samples collected from Lake Taihu, China. MC-LR, MC-YR and MC-RR were 
      detected in the range of 0.5-3.1 mug/kg. The experimental results proved the 
      feasibility of the developed method for the identification and quantification of 
      various MCs in fish products.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Xu, Xiaoying
AU  - Xu X
AD  - Zhejiang Institute for Food and Drug Control, Hangzhou 310000, China. Electronic 
      address: net_easexxy@126.com.
FAU - Zhu, Bingqi
AU  - Zhu B
AD  - College of Medical Technology, Zhejiang Chinese Medical University, Hangzhou 
      310000, China.
FAU - Liu, Zhu
AU  - Liu Z
AD  - Zhejiang Institute for Food and Drug Control, Hangzhou 310000, China.
FAU - Wang, Feng
AU  - Wang F
AD  - Zhejiang Institute for Food and Drug Control, Hangzhou 310000, China.
FAU - Liang, Jingjing
AU  - Liang J
AD  - Zhejiang Institute for Food and Drug Control, Hangzhou 310000, China.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20190710
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Microcystins)
RN  - 77238-39-2 (microcystin)
SB  - IM
MH  - Animals
MH  - China
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Fishes
MH  - Food Contamination/analysis
MH  - Limit of Detection
MH  - Microcystins/*chemistry/*isolation & purification
MH  - Seafood/analysis
MH  - Solid Phase Extraction/*methods
MH  - Tandem Mass Spectrometry/*methods
OTO - NOTNLM
OT  - Fish
OT  - Microcystins
OT  - Online SPE-UHPLC-MS/MS
OT  - PRiME
EDAT- 2019/07/20 06:00
MHDA- 2019/12/18 06:00
CRDT- 2019/07/20 06:00
PHST- 2019/03/27 00:00 [received]
PHST- 2019/06/23 00:00 [revised]
PHST- 2019/06/27 00:00 [accepted]
PHST- 2019/07/20 06:00 [pubmed]
PHST- 2019/12/18 06:00 [medline]
PHST- 2019/07/20 06:00 [entrez]
AID - S1570-0232(19)30514-8 [pii]
AID - 10.1016/j.jchromb.2019.06.036 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2019 Sep 1;1125:121709. doi: 
      10.1016/j.jchromb.2019.06.036. Epub 2019 Jul 10.