PMID- 31323756
OWN - NLM
STAT- MEDLINE
DCOM- 20200406
LR  - 20200408
IS  - 2218-273X (Electronic)
IS  - 2218-273X (Linking)
VI  - 9
IP  - 7
DP  - 2019 Jul 18
TI  - Alleviated Oxidative Damage by Taraxacum officinale through the Induction of 
      Nrf2-MAPK/PI3K Mediated HO-1 Activation in Murine Macrophages RAW 264.7 Cell 
      Line.
LID - 10.3390/biom9070288 [doi]
LID - 288
AB  - Taraxacum officinale has been consumed as a folk remedy due to its diverse 
      physiological activities. This study aimed to investigate the antioxidative 
      potential of T. officinale water extract (TOWE) and ethanol extract (TOEE) 
      against oxidative stress and compare their molecular mechanism via the induction 
      of heme oxygenase-1 (HO-1) in RAW 264.7 cells. The antioxidative activity was 
      evaluated through the radical scavenging assay, the cytoprotection assay against 
      oxidative damage, and Western blot analysis. Both extracts dose-dependently 
      induced HO-1 expression without any cytotoxicity in accordance with the 
      activation of a transcription factor, nuclear factor-erythroid 2 p45-related 
      factor 2 (Nrf2). In addition, TOWE induced HO-1 expression through the 
      phosphorylation of phosphoinositide 3-kinase (PI3K)/Akt and c-Jun NH(2)-terminal 
      kinase (JNK), while TOEE activated HO-1 by PI3K/Akt phosphorylation. In order to 
      identify the antioxidative potential by HO-1 induction, oxidative damage-caused 
      cell death by tert-butyl hydroperoxide (t-BHP) was significantly attenuated by 
      both extracts. Their antioxidative potential was confirmed by HO-1 selective 
      inducer and inhibitor, cobalt protoporphyrin (CoPP), and tin protoporphyrin 
      (SnPP), respectively. These results indicate that TOWE and TOEE potently 
      alleviated oxidative damage via the induction of Nrf2/MAPK/PI3K mediated HO-1 
      induction in RAW 264.7 cells.
FAU - Yoon, Hyun-Seo
AU  - Yoon HS
AD  - Department of Dental Hygiene, Dong-Eui University, Busan 47340, Korea.
FAU - Park, Chung Mu
AU  - Park CM
AD  - Department of Clinical Laboratory Science, Dong-Eui University, Busan 47340, 
      Korea. cmpark@deu.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20190718
PL  - Switzerland
TA  - Biomolecules
JT  - Biomolecules
JID - 101596414
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (Plant Extracts)
RN  - 0 (Reactive Oxygen Species)
SB  - IM
MH  - Animals
MH  - Mice
MH  - NF-E2-Related Factor 2/*metabolism
MH  - Oxidative Stress/drug effects
MH  - Phosphatidylinositol 3-Kinases/*metabolism
MH  - Plant Extracts/*pharmacology
MH  - RAW 264.7 Cells
MH  - Reactive Oxygen Species/metabolism
MH  - Signal Transduction/drug effects
MH  - Taraxacum/*chemistry
PMC - PMC6681201
OTO - NOTNLM
OT  - Heme Oxygenase-1
OT  - Mitogen-Activated Protein Kinases
OT  - NF-E2-Related Factor 2
OT  - Taraxacum officinale
COIS- The authors declare no conflict of interest.
EDAT- 2019/07/22 06:00
MHDA- 2020/04/09 06:00
PMCR- 2019/07/01
CRDT- 2019/07/21 06:00
PHST- 2019/05/30 00:00 [received]
PHST- 2019/07/11 00:00 [revised]
PHST- 2019/07/15 00:00 [accepted]
PHST- 2019/07/21 06:00 [entrez]
PHST- 2019/07/22 06:00 [pubmed]
PHST- 2020/04/09 06:00 [medline]
PHST- 2019/07/01 00:00 [pmc-release]
AID - biom9070288 [pii]
AID - biomolecules-09-00288 [pii]
AID - 10.3390/biom9070288 [doi]
PST - epublish
SO  - Biomolecules. 2019 Jul 18;9(7):288. doi: 10.3390/biom9070288.