PMID- 31325880
OWN - NLM
STAT- MEDLINE
DCOM- 20191101
LR  - 20191101
IS  - 1873-6424 (Electronic)
IS  - 0269-7491 (Linking)
VI  - 253
DP  - 2019 Oct
TI  - Establishment of a multiplex RT-PCR assay for identification of atmospheric virus 
      contamination in pig farms.
PG  - 358-364
LID - S0269-7491(19)32053-6 [pii]
LID - 10.1016/j.envpol.2019.07.028 [doi]
AB  - Spread of pathogens in pig farms not only causes transfection of diseases to 
      other pigs or even farmers working in the farms, but also induces pollution to 
      the living atmospheric environment of the residents around the farm. Therefore, 
      it is necessary to establish a rapid and simple monitoring method. In this study, 
      full genome sequences of common viruses were analyzed in pig farms, in 
      combination with the design of primers, optimization of the reaction parameters, 
      so as to establish a multiplex RT-PCR assay for the identification of classical 
      swine fever virus (CSFV), Japanese encephalitis virus (JEV), porcine reproductive 
      and respiratory syndrome virus (PRRSV), porcine circovirus Type 2 (PCV-2), 
      porcine pseudorabies virus (PRV) and porcine parvovirus virus (PPV), which are 
      common in pig farms. This method has a minimal detectable concentration of 
      10(-3) ng/muL, which is highly specific. Furthermore, multiplex RT-PCR was applied 
      to examine air samples from 4 pig farms located in different cities of China. The 
      results were in line with those obtained by single PCR. Therefore, this study can 
      be expected to provide essential technique support for the early warning 
      mechanism as well as disease prevention and control system against the major 
      viruses.
CI  - Copyright (c) 2019 Elsevier Ltd. All rights reserved.
FAU - Li, Han
AU  - Li H
AD  - Henan Institute of Science and Technology, Xinxiang 453003, Henan, PR China. 
      Electronic address: lihanenjoy@hist.edu.cn.
FAU - Wei, Xiaobing
AU  - Wei X
AD  - Henan Institute of Science and Technology, Xinxiang 453003, Henan, PR China.
FAU - Zhang, Xiulin
AU  - Zhang X
AD  - Henan Institute of Science and Technology, Xinxiang 453003, Henan, PR China.
FAU - Xu, Hao
AU  - Xu H
AD  - School of Environment and Energy, South China University of Technology, Guangzhou 
      510006, PR China.
FAU - Zhao, Xuesong
AU  - Zhao X
AD  - School of Environment and Energy, South China University of Technology, Guangzhou 
      510006, PR China.
FAU - Zhou, Shaofeng
AU  - Zhou S
AD  - School of Environmental Science and Engineering, Guangdong University of 
      Technology, Guangzhou 510006, PR China.
FAU - Huang, Shaobin
AU  - Huang S
AD  - School of Environment and Energy, South China University of Technology, Guangzhou 
      510006, PR China.
FAU - Liu, Xingyou
AU  - Liu X
AD  - Xinxiang University, Xinxiang 453003, Henan, PR China.
LA  - eng
PT  - Journal Article
DEP - 20190710
PL  - England
TA  - Environ Pollut
JT  - Environmental pollution (Barking, Essex : 1987)
JID - 8804476
RN  - 0 (Air Pollutants)
RN  - 0 (DNA Primers)
SB  - IM
MH  - *Air Microbiology
MH  - Air Pollutants/*analysis
MH  - Animals
MH  - China
MH  - Circovirus
MH  - DNA Primers
MH  - *Environmental Monitoring
MH  - Farms
MH  - Multiplex Polymerase Chain Reaction
MH  - Porcine respiratory and reproductive syndrome virus
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sensitivity and Specificity
MH  - Swine
MH  - Swine Diseases/virology
MH  - *Viruses
OTO - NOTNLM
OT  - Atmospheric environment
OT  - Monitoring method
OT  - Multiplex RT-PCR assay
OT  - Pathogenic microorganism
EDAT- 2019/07/22 06:00
MHDA- 2019/11/02 06:00
CRDT- 2019/07/21 06:00
PHST- 2019/04/18 00:00 [received]
PHST- 2019/06/20 00:00 [revised]
PHST- 2019/07/06 00:00 [accepted]
PHST- 2019/07/22 06:00 [pubmed]
PHST- 2019/11/02 06:00 [medline]
PHST- 2019/07/21 06:00 [entrez]
AID - S0269-7491(19)32053-6 [pii]
AID - 10.1016/j.envpol.2019.07.028 [doi]
PST - ppublish
SO  - Environ Pollut. 2019 Oct;253:358-364. doi: 10.1016/j.envpol.2019.07.028. Epub 
      2019 Jul 10.