PMID- 31326654
OWN - NLM
STAT- MEDLINE
DCOM- 20200121
LR  - 20210110
IS  - 1872-9142 (Electronic)
IS  - 0161-5890 (Print)
IS  - 0161-5890 (Linking)
VI  - 114
DP  - 2019 Oct
TI  - Comparative structural and thermal stability studies of Cuc m 2.0101, Art v 
      4.0101 and other allergenic profilins.
PG  - 19-29
LID - S0161-5890(19)30169-5 [pii]
LID - 10.1016/j.molimm.2019.07.004 [doi]
AB  - Worldwide, more than one-third of the population suffers from allergies. A 
      significant fraction of officially registered allergens originate from the 
      profilin family of proteins. Profilins are small ubiquitous proteins which are 
      found in plants, viruses and various eukaryotes including mammals. Although they 
      are primarily regarded as minor allergens, profilins are important players in 
      immunoglobulin E (IgE) cross-reactivity. However, in some populations profilins 
      are recognized by IgE from at least 50% of patients allergic to a given allergen 
      source. Cuc m 2.0101 is recognized by IgE in more than 80% of muskmelon-allergic 
      patients. The recombinant isoallergen Cuc m 2.0101 was produced in significant 
      quantities and its X-ray crystal structure was determined. In addition, a new Art 
      v 4.0101 (mugwort profilin) structure was determined. The profilins Cuc m 2.0101 
      and Art v 4.0101 were compared in terms of their structure and thermal stability. 
      Furthermore, structural similarities and IgE cross-reactivity between profilins 
      from different sources are discussed to explain the molecular basis of various 
      clinical syndromes involving this group of allergens. Special emphasis is placed 
      on discussion of profilins' quaternary structures and their relation to 
      biological function, as well as to protein allergenicity. Moreover, a potential 
      impact of protein purification protocols on the structure of profilins is 
      highlighted.
CI  - Copyright (c) 2019 Elsevier Ltd. All rights reserved.
FAU - Kapingidza, A Brenda
AU  - Kapingidza AB
AD  - Department of Chemistry and Biochemistry, University of South Carolina, Columbia, 
      SC, 29208, United States.
FAU - Pye, Sarah E
AU  - Pye SE
AD  - Department of Chemistry and Biochemistry, University of South Carolina, Columbia, 
      SC, 29208, United States.
FAU - Hyduke, Noah
AU  - Hyduke N
AD  - Department of Chemistry and Biochemistry, University of South Carolina, Columbia, 
      SC, 29208, United States.
FAU - Dolamore, Coleman
AU  - Dolamore C
AD  - Department of Chemistry and Biochemistry, University of South Carolina, Columbia, 
      SC, 29208, United States.
FAU - Pote, Swanandi
AU  - Pote S
AD  - Department of Chemistry and Biochemistry, University of South Carolina, Columbia, 
      SC, 29208, United States.
FAU - Schlachter, Caleb R
AU  - Schlachter CR
AD  - Department of Chemistry and Biochemistry, University of South Carolina, Columbia, 
      SC, 29208, United States.
FAU - Commins, Scott P
AU  - Commins SP
AD  - Thurston Arthritis Research Center, University of North Carolina at Chapel Hill, 
      NC, 27599, United States.
FAU - Kowal, Krzysztof
AU  - Kowal K
AD  - Department of Allergology and Internal Medicine, Medical University of Bialystok, 
      Bialystok, Poland; Department of Experimental Allergology and Immunology, Medical 
      University of Bialystok, Bialystok, Poland.
FAU - Chruszcz, Maksymilian
AU  - Chruszcz M
AD  - Department of Chemistry and Biochemistry, University of South Carolina, Columbia, 
      SC, 29208, United States. Electronic address: chruszcz@mailbox.sc.edu.
LA  - eng
GR  - R01 AI077653/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20190718
PL  - England
TA  - Mol Immunol
JT  - Molecular immunology
JID - 7905289
RN  - 0 (Antigens, Plant)
RN  - 0 (Plant Proteins)
RN  - 0 (Profilins)
RN  - 0 (Recombinant Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antigens, Plant/*chemistry/immunology
MH  - Cross Reactions/immunology
MH  - Escherichia coli/immunology/metabolism
MH  - Hypersensitivity/immunology
MH  - Immunoglobulin E/chemistry
MH  - Plant Proteins/chemistry/immunology
MH  - Pollen/chemistry/immunology
MH  - Profilins/*chemistry/immunology
MH  - Recombinant Proteins/chemistry/immunology
PMC - PMC6774812
MID - NIHMS1535128
OTO - NOTNLM
OT  - Allergen
OT  - Oligomerization
OT  - Pollen-food syndrome
OT  - Profilin
OT  - Thermal stability
EDAT- 2019/07/22 06:00
MHDA- 2020/01/22 06:00
PMCR- 2020/10/01
CRDT- 2019/07/22 06:00
PHST- 2019/03/04 00:00 [received]
PHST- 2019/06/25 00:00 [revised]
PHST- 2019/07/08 00:00 [accepted]
PHST- 2019/07/22 06:00 [pubmed]
PHST- 2020/01/22 06:00 [medline]
PHST- 2019/07/22 06:00 [entrez]
PHST- 2020/10/01 00:00 [pmc-release]
AID - S0161-5890(19)30169-5 [pii]
AID - 10.1016/j.molimm.2019.07.004 [doi]
PST - ppublish
SO  - Mol Immunol. 2019 Oct;114:19-29. doi: 10.1016/j.molimm.2019.07.004. Epub 2019 Jul 
      18.