PMID- 3132714
OWN - NLM
STAT- MEDLINE
DCOM- 19880725
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 85
IP  - 12
DP  - 1988 Jun
TI  - Regulation of interleukin 2 receptor alpha subunit (Tac or CD25 antigen) gene 
      expression: binding of inducible nuclear proteins to discrete promoter sequences 
      correlates with transcriptional activation.
PG  - 4468-72
AB  - Transfection of deleted forms of the human interleukin 2 receptor alpha subunit 
      (IL-2R alpha; also called CD25 or Tac antigen) gene (IL2RA) promoter revealed a 
      requirement for sequences 3' of base -317 for phytohemagglutinin- and phorbol 
      12-myristate 13-acetate (PMA)-induced promoter activation in CD4+ Jurkat T cells. 
      In contrast, sequences 3' of base -271 were sufficient for promoter induction in 
      CD4-/CD8- YT-1 T cells or Jurkat cells expressing the transactivator protein 
      (tat-I) of human T-cell lymphotropic virus type I (HTLV-I). Gel retardation 
      assays revealed that nuclear extracts from induced, but not uninduced, Jurkat and 
      YT-1 cells mediated the formation of two specific DNA-protein complexes with 
      oligonucleotides spanning the region of the IL2RA promoter from position -291 to 
      -245, which contains two imperfect direct repeats (IDRs). Consistent with the 
      different 5' sequence requirements for promoter activation in Jurkat and YT-1 
      cells, oligonucleotides corresponding to the region from -267 to -243 (downstream 
      IDR and flanking region) formed only one complex with induced Jurkat extracts but 
      two complexes with induced YT-1 extracts. Oligonucleotides containing the region 
      of the IL2RA promoter from -293 to -270 (upstream IDR and flanking region) failed 
      to bind protein in either cell type. In further support of the biological 
      significance of these DNA-protein interactions, the IL2RA oligonucleotide from 
      -291 to -245 proved to be sufficient in either orientation to confer PMA 
      inducibility to the mitogen-insensitive thymidine kinase gene promoter in Jurkat 
      cells. Together, these findings suggest that the interaction of inducible DNA 
      binding proteins with the IL2RA promoter between bases -291 and -245 plays an 
      important role in mitogen-induced changes in the transcriptional activity of this 
      receptor gene. Furthermore, the requisite 5' sequences appear to differ in T 
      cells depending upon the nature of the activation signal and perhaps the stage of 
      cellular maturation.
FAU - Lowenthal, J W
AU  - Lowenthal JW
AD  - Howard Hughes Medical Institute, Department of Medicine, Duke University Medical 
      Center, Durham 27710.
FAU - Bohnlein, E
AU  - Bohnlein E
FAU - Ballard, D W
AU  - Ballard DW
FAU - Greene, W C
AU  - Greene WC
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Interleukin-2)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Receptors, Interleukin-2)
SB  - IM
MH  - Base Sequence
MH  - Chromosome Deletion
MH  - *Gene Expression Regulation
MH  - *Genes
MH  - Genetic Vectors
MH  - Humans
MH  - Interleukin-2/*immunology
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/metabolism
MH  - Promoter Regions, Genetic
MH  - Receptors, Immunologic/*genetics
MH  - Receptors, Interleukin-2
MH  - *Transcription, Genetic
PMC - PMC280451
EDAT- 1988/06/01 00:00
MHDA- 1988/06/01 00:01
PMCR- 1988/12/01
CRDT- 1988/06/01 00:00
PHST- 1988/06/01 00:00 [pubmed]
PHST- 1988/06/01 00:01 [medline]
PHST- 1988/06/01 00:00 [entrez]
PHST- 1988/12/01 00:00 [pmc-release]
AID - 10.1073/pnas.85.12.4468 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1988 Jun;85(12):4468-72. doi: 10.1073/pnas.85.12.4468.